Team:TzuChiU Formosa/Notebook/photopaper

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<font color="#228B22" size=6>Photopaper</font>
 +
<br><br><font color="#000080" size=4>Meeting Minutes</font>
 +
<Hr Align="left" width="100%" size=2>
 +
<font color="#000000" size=3><b>2011.02.24</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
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*Team organization[[File:001.jpg|right|500px|caption]]
 +
*Brain storming
 +
**paper made by bacteria with add-ons such as colors, fragrance, etc.
 +
**"light up" the plants for replacing lamp posts.
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.03.04</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
*Team advisory 
 +
*Brain storming
 +
**Add-ons: colorful cellulose which produced by bacteria such as beta-carotene
 +
**information exchange with iGEM 2009 Cambridge team
 +
 
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.03.14</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
*Task Allocation
 +
*Brain storming
 +
**Avatar - "light up" the plants by transfecting symbiotic bacteria which cloned into fluorescence gene
 +
**Eco-friendly warmer - biotic thermal pad
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.03.23</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
**Project : paperia
 +
**Option 1 : Culture bacteria which has pigment gene
 +
**Option 2 : Cellulose-producing bacteria secrete pigment into the medium
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.03.24</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
* Exp. procedure:
 +
** cloning of cellulose gene’s CDS
 +
** the product should operate within E. coli.
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.06.22</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
* Due to some unforseen reason, the team decided to change their project.
 +
* New project: Biojenny
 +
　　　　　　　　　-economical and humane way to produce paper in large quantities.<br>
 +
　　　　　　　　　-yeast to be our host
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.07.01</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
* Freeze > grin > genome DNA isolation > Cloning = silk protein gene
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.07.09</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
* the connections between 3 silk proteins : Fibl Fibh P25
 +
* major proteins : H-chain, L-chain, P25
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.07.15</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
* Due to limited resources and techniques required, the team decided to switch back to the previous project. Paper making !
 +
* However it would be modified to be more innovative and creative.
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.07.18</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
* Latest project : Photo paper
 +
* cyanobacteria is designed as the host, cellulose made up of the glucose produced by cyanobacteria could be one of the main attraction of the project.
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.07.23</b></font>
 +
<br><font size=2><b>Discussion:</b></font>
 +
 
 +
* system modification to overcome the problems arises during preliminary round
 +
* Biobricks from Tokyo 2010 team will be utilized
 +
**regulator promoter in order to regulate the secretion of cellulose to solve the aggregation of the bacteria
 +
 
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.09.15</b></font>
 +
 
 +
'''Genome miniprep'''<br>
 +
Gluconacetobacter hansenii
 +
 
 +
 
 +
 
 +
<br><font color="#000000" size=3><b>2011.09.18</b></font>
 +
 
 +
'''Gel/PCR DNA extraction'''<br>
 +
Gluconacetobacter hansenii
 +
 
 +
 
 +
 
 +
<font size=4 color="#000080"><b>Protocols</b></font>
 +
<Hr Align="left" width="100%" size=3>
 +
 
 +
 
 +
<br>[[File:1-20110907.jpg|lefe|650px|caption]][[File:Catt.gif|right|250px|caption]]
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<br>
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<br>
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<br>[[File:2-20110908-13.jpg|lefe|650px|caption]]
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<br>
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<br>[[File:3-20110910-11.jpg|lefe|650px|caption]]
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<br>
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<br>
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<br>[[File:4-20110912-20.jpg|lefe|650px|caption]]
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<br>
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<br>
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<br>[[File:5-20110921.jpg|lefe|550px|caption]][[File:Cats.jpg|right|404px|caption]]
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<br>
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<br>
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<br>
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<br>[[File:6-20110922.jpg|lefe|650px|caption]]
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<br>
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<br>[[File:7-20110923.jpg|lefe|650px|caption]]
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<br>[[File:8-20110924.jpg|lefe|550px|caption]][[File:Cats2.gif |right|404px|caption]]
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<br>
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<br>
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<br>
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<br>[[File:9-20110925.jpg|lefe|650px|caption]]
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<br>
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<br>[[File:10-20110926.jpg|lefe|650px|caption]]
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<br>[[File:11-20110927.jpg|lefe|550px|caption]][[File:Cats3.jpg|right|404px|caption]]
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<br>
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<br>
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<br>[[File:12-20110928.jpg|lefe|650px|caption]]
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<br>
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<br>[[File:13-20110929.jpg|lefe|650px|caption]]
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<br>
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<br>[[File:14-20111001.jpg|lefe|650px|caption]]
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Latest revision as of 02:56, 6 October 2011

Photopaper

Meeting Minutes

2011.02.24
Discussion:

  • Team organization
    caption
  • Brain storming
    • paper made by bacteria with add-ons such as colors, fragrance, etc.
    • "light up" the plants for replacing lamp posts.



2011.03.04
Discussion:

  • Team advisory
  • Brain storming
    • Add-ons: colorful cellulose which produced by bacteria such as beta-carotene
    • information exchange with iGEM 2009 Cambridge team




2011.03.14
Discussion:

  • Task Allocation
  • Brain storming
    • Avatar - "light up" the plants by transfecting symbiotic bacteria which cloned into fluorescence gene
    • Eco-friendly warmer - biotic thermal pad



2011.03.23
Discussion:

    • Project : paperia
    • Option 1 : Culture bacteria which has pigment gene
    • Option 2 : Cellulose-producing bacteria secrete pigment into the medium



2011.03.24
Discussion:

  • Exp. procedure:
    • cloning of cellulose gene’s CDS
    • the product should operate within E. coli.



2011.06.22
Discussion:

  • Due to some unforseen reason, the team decided to change their project.
  • New project: Biojenny

　　　　　　　　　-economical and humane way to produce paper in large quantities.
　　　　　　　　　-yeast to be our host



2011.07.01
Discussion:

  • Freeze > grin > genome DNA isolation > Cloning = silk protein gene



2011.07.09
Discussion:

  • the connections between 3 silk proteins : Fibl Fibh P25
  • major proteins : H-chain, L-chain, P25



2011.07.15
Discussion:

  • Due to limited resources and techniques required, the team decided to switch back to the previous project. Paper making !
  • However it would be modified to be more innovative and creative.



2011.07.18
Discussion:

  • Latest project : Photo paper
  • cyanobacteria is designed as the host, cellulose made up of the glucose produced by cyanobacteria could be one of the main attraction of the project.



2011.07.23
Discussion:

  • system modification to overcome the problems arises during preliminary round
  • Biobricks from Tokyo 2010 team will be utilized
    • regulator promoter in order to regulate the secretion of cellulose to solve the aggregation of the bacteria




2011.09.15

Genome miniprep
Gluconacetobacter hansenii



2011.09.18

Gel/PCR DNA extraction
Gluconacetobacter hansenii


Protocols



caption
caption





caption



caption



caption



caption
caption





caption



caption



caption
caption





caption



caption



caption
caption





caption



caption



caption


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