Team:Peking S/lab/notebook/myr
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+ | {{Template:Http://2011.igem.org/Team:Peking S/box4notebook}} | ||
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+ | <font size=6> <font color="#FFFFFF">Yanrong Ma's Notebook</font></FONT> | ||
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+ | == '''summary''' == | ||
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+ | In our project I cloned genes of A-factor synthase afsA and A-factor receptor arpA,and I also standardized these two genes .Besides, I also constructed and characterized quorum sensing genes named cinI and cinR. I further characterized its feasibilities in constructing synthetic microbial consortia. | ||
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+ | =='''Contents'''== | ||
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6.24-7.5 | 6.24-7.5 | ||
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Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion. | Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion. | ||
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7.6-7.7 | 7.6-7.7 | ||
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Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing. | Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing. | ||
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7.8-7.12 | 7.8-7.12 | ||
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Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome. | Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome. | ||
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7.13-7.23 | 7.13-7.23 | ||
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Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters. | Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters. | ||
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7.24-8.13 | 7.24-8.13 | ||
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Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules. | Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules. | ||
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8.14-9.5 | 8.14-9.5 | ||
- | Testing | + | |
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+ | Testing performance of afsA/arpA system. | ||
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9.6-9.18 | 9.6-9.18 | ||
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+ | Constructing cinI/cinR system which mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules. | ||
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9.19-10.1 | 9.19-10.1 | ||
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+ | Characterizing of cinI/cinR system. |
Latest revision as of 05:21, 2 October 2011
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Yanrong Ma's Notebook
summary
In our project I cloned genes of A-factor synthase afsA and A-factor receptor arpA,and I also standardized these two genes .Besides, I also constructed and characterized quorum sensing genes named cinI and cinR. I further characterized its feasibilities in constructing synthetic microbial consortia.
Contents
6.24-7.5
Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion.
7.6-7.7
Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing.
7.8-7.12
Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome.
7.13-7.23
Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters.
7.24-8.13
Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules.
8.14-9.5
Testing performance of afsA/arpA system.
9.6-9.18
Constructing cinI/cinR system which mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules.
9.19-10.1
Characterizing of cinI/cinR system.