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Team:IIT Madras/Notebook/Protocols/Transformation
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Latest revision as of 12:56, 2 October 2011
IIT Madras
And then the E.coli said, "Let there be light"
Transformation
- Add 2 ul of Plasmid DNA to competent cells (100 ul aliquot) on ice for 30 minutes.
- Heat shock : 90 s for 43 C.
- Keep on ice for 2 minutes.
- Add 900 ul of LB broth (sterile) into each microfuge tubes.
- Incubate in shaker at 37 C for 1 hour.
- Plate on plates (with antibiotics if necessary) and incubate at 37 C overnight.
