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Team:Panama/15 August 2011
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(Created page with " ==August 15, 2011== '''We did:''' 1-Preparation of TAE 1X. 2. Electrophoresis in TAE 1X with products of PCR with Rh1AB primers (8/14/11). 3.Autoclave LB medium for competen...") |
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2. Electrophoresis in TAE 1X with products of PCR with Rh1AB primers (8/14/11). | 2. Electrophoresis in TAE 1X with products of PCR with Rh1AB primers (8/14/11). | ||
| - | 3.Autoclave LB medium for competent cells. | + | 3. Autoclave LB medium for competent cells. |
| - | 4.Verified growth of bacteria (Not growth appropriately). | + | 4. Verified growth of bacteria (Not growth appropriately). |
5. Reformulate protocol for competent cells. | 5. Reformulate protocol for competent cells. | ||
| + | |||
| + | 6. Concentrate DNA of PCR of 8/14/11 - 50 microliters each tube approximately. | ||
| + | |||
| + | 7. Prepare materials for competent cell. | ||
| + | |||
| + | 8. Cells overnight | ||
Latest revision as of 03:45, 29 September 2011
August 15, 2011
We did:
1-Preparation of TAE 1X.
2. Electrophoresis in TAE 1X with products of PCR with Rh1AB primers (8/14/11).
3. Autoclave LB medium for competent cells.
4. Verified growth of bacteria (Not growth appropriately).
5. Reformulate protocol for competent cells.
6. Concentrate DNA of PCR of 8/14/11 - 50 microliters each tube approximately.
7. Prepare materials for competent cell.
8. Cells overnight
