Team:Calgary/Notebook/Protocols/Process20
From 2011.igem.org
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<li> Overnight bacterial growth </li> | <li> Overnight bacterial growth </li> | ||
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<li> Place in -80°C freezer when frozen. </li> | <li> Place in -80°C freezer when frozen. </li> | ||
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Latest revision as of 04:13, 29 September 2011
Preparing Glycerol Stocks
Reagents and Materials
- Overnight bacterial growth
- Screw Cap Tubes
- Glycerol
Protocol
- Pipet 0.5mL of 50% glycerol into 3 1.5mL screw cap tubes
- Add 0.5mL of overnight culture to each tube.
- Pipet up and down to gently mix.
- Flash freeze in liquid N2 or dry ice/ethanol bath.
- Place in -80°C freezer when frozen.