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From 2011.igem.org

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-	We take a sample of rh1AB purifying gen without mutation and doing a PCR because doesn’t have a lot and we have to digest with Pst1. On Monday we are going to do a mutagenesis.	+	==22 Septembre 2011==
-	We use the PCR protocol that functions well with our gene ([https://2011.igem.org/PCR'''PCR Protocol''']) and we test all primers because we don’t know which primers the team used the last year for genes amplification.	+	'''We did:'''
-	[[File:tabla7.png|700px|thumb|left|alt text]]	+	1. Broth of XL10 gold (50 microliters in 2 ml of LB).
-		+	2. Was supplemented the NZCYM of sigma adding compounds to maik it NZY+.
-		+
-	Primers:	+
-		+
-	1. F:RhTf2a R:RhTR2a	+
-		+
-	2. F:RhT-f1b R:RhT-2b	+
-		+
-	3. F:RhTBio-R2a R:RhTBio-F1a	+
-		+
-	4. F:RhTBio-f1 R:RhTBio-2b	+
-		+
-		+
-	Meanwhile Orlando and Paul make the miniprep of (RBS + P) and (GFP + T) using the protocol of dirty miniprep on page 80.Right Now Sergio and Natasha take the miniprep results and check the concentration with Nanodrop while Paul prepares PCR.	+
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-	RESULTS:	+
-	[[File:tabla8.png|700px|thumb|left|alt text]]	+
-		+
-	We have good levels of absorbance and concentration in the samples, we hope that the electrophoresis be good.	+

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Latest revision as of 04:18, 29 September 2011

22 Septembre 2011

We did:

1. Broth of XL10 gold (50 microliters in 2 ml of LB).

2. Was supplemented the NZCYM of sigma adding compounds to maik it NZY+.