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Team:UT Dallas/data
From 2011.igem.org
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<h2>Data</h2> | <h2>Data</h2> | ||
| - | + | <b>How our system works</b><br> | |
| - | <b>Data For Our Favorite New Parts</b | + | <img src="https://static.igem.org/mediawiki/2011/1/1b/Schematic.png" style="border 1px solid"><br><br> |
| - | <br>PcstA-RBS-LuxI-terminator, BBa_K569001 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569001">Main Page</a>): This part produces LuxI,an enzyme for creating acyl-homoserine lactones from normal cell metabolites, in the absence of glucose. This part is repressed by glucose. | + | <b>Data For Our Favorite New Parts</b> |
| - | <br>PyeaR-ToxR-FGFR, BBa_K569013 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569013">Main Page</a>): PyeaR is a nitrate inducible promoter. ToxR+FGFR is a receptor fusion of two dimerizing proteins. FGFR responds to FGF by dimerizing; this causes ToxR to dimerize. When ToxR is dimerized it acts as a transcription factor for the ctx promoter. | + | <br>PcstA-RBS-LuxI-terminator, BBa_K569001 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569001">Main Page</a>): This part produces LuxI,an enzyme for creating acyl-homoserine lactones from normal cell metabolites, in the absence of glucose. This part is repressed by glucose.<br> |
| + | <br>PyeaR-ToxR-FGFR, BBa_K569013 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569013">Main Page</a>): PyeaR is a nitrate inducible promoter. ToxR+FGFR is a receptor fusion of two dimerizing proteins. FGFR responds to FGF by dimerizing; this causes ToxR to dimerize. When ToxR is dimerized it acts as a transcription factor for the ctx promoter. <br> | ||
<br>SCP-ToxR-FGFR, BBa_K569014 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569014">Main Page</a>): This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.<br><br> | <br>SCP-ToxR-FGFR, BBa_K569014 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569014">Main Page</a>): This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.<br><br> | ||
<b>Data For Pre-existing Parts</b><br> | <b>Data For Pre-existing Parts</b><br> | ||
| - | + | We grew PcstA-RBS-LuxI-terminator and AHL-inducible ColicinE2-GFP transformed in BL21 E.coli cells. We combined the two cultures in various ratios and either added or did not add glucose (<a href="http://partsregistry.org/Part:BBa_K131010:Experience">experience</a>). We allowed these samples to continue growing for 2 hours and we took measurements using a fluorescent microscope. | |
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Latest revision as of 05:33, 29 September 2011
Data
How our system works
Data For Our Favorite New Parts
PcstA-RBS-LuxI-terminator, BBa_K569001 (Main Page): This part produces LuxI,an enzyme for creating acyl-homoserine lactones from normal cell metabolites, in the absence of glucose. This part is repressed by glucose.
PyeaR-ToxR-FGFR, BBa_K569013 (Main Page): PyeaR is a nitrate inducible promoter. ToxR+FGFR is a receptor fusion of two dimerizing proteins. FGFR responds to FGF by dimerizing; this causes ToxR to dimerize. When ToxR is dimerized it acts as a transcription factor for the ctx promoter.
SCP-ToxR-FGFR, BBa_K569014 (Main Page): This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.
Data For Pre-existing Parts
We grew PcstA-RBS-LuxI-terminator and AHL-inducible ColicinE2-GFP transformed in BL21 E.coli cells. We combined the two cultures in various ratios and either added or did not add glucose (experience). We allowed these samples to continue growing for 2 hours and we took measurements using a fluorescent microscope.
