Team:Grinnell/Notebook/Gels

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<h2>Gel Pictures</h2>
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<h1>Gel Pics</h1>
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<h3>June 6 to 10</h3>
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<img alt="Our first gel" src='https://static.igem.org/mediawiki/2011/3/32/20110606_PCRproduct.jpg' height=200px />
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<p>We collected more gel data than we could fit on one page. Please go on to one of the following pages:</p>
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<a name="20110606_PCRproduct" href="https://2011.igem.org/File:20110606_PCRproduct.jpg"><img alt="PCR product from 20110606 showing that PCR of rsaA C-terminal and esp was successful" src="https://static.igem.org/mediawiki/2011/3/32/20110606_PCRproduct.jpg" width=200px /></a>
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    <li><a href="https://2011.igem.org/Team:Grinnell/Notebook/Gels/DspB">DspB</a></li>
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    <li><a href="https://2011.igem.org/Team:Grinnell/Notebook/Gels/Esp">Esp</a></li>
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PCR products on DNA gel. Lane 1: ladder; Lane 2: <i>rsaA</i> from liquid culture <i>Caulobacter</i>; Lane 3: <i>rsaA</i> from plate culture <i>Caulobacter</i>; Lane 4: <i>esp</i> from <i>S. epidermidis</i>.
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    <li><a href="https://2011.igem.org/Team:Grinnell/Notebook/Gels/Promoters">Promoters</a></li>
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<a name="20110610PlamidGel" href="https://2011.igem.org/File:20110610PlamidGel.jpg"><img alt="Gel proving successful insertion of rsaA C-terminal and esp into destination plasmid" src="https://static.igem.org/mediawiki/2011/a/a5/20110610PlamidGel.jpg" width=200px /></a>
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Gel results for transformation of ligations. Lane 1: ladder; Lane 2: digested plasmid with <i>rsaA</i> C-terminal; Lane 3: digested plasmid with <i>rsaA</i> C-terminal and <i>esp</i>; Lanes 4-8: digested plasmids from various colonies with <i>esp</i>.
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<h3>June 13 to 17</h3>
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<a name="20110614_PCR135" href="https://2011.igem.org/File:20110614_PCR135.jpg"><img alt="Gel showing colony PCR products of transformation products of various ligations of rsaA C-terminal and esp" src="https://static.igem.org/mediawiki/2011/1/1f/20110614_PCR135.jpg" width=200px /></a>
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Gel 1 of 2. Lane 1: ladder; lanes 2-4: PCR product using VF2 and VR of plasmid containing <i>esp</i> ligated with <i>rsaA</i> C-terminal that had been digested 20110606; lane 5: PCR product of plasmid containing <i>esp</i>; lanes 6-8: PCR product using VF2 and VR of plasmid containing <i>esp</i> ligated with <i>rsaA</i> C-terminal PCR product from 20110605. Only lane 6 shows successful ligation of <i>esp</i> and <i>rsaA</i> C-terminal.
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<a name="20110614_PCR246" href="https://2011.igem.org/File:20110614_PCR246.jpg"><img alt="Gel 2 of 2 for 20110614 for Alex and Qimeng" src="https://static.igem.org/mediawiki/2011/1/1d/20110614_PCR246.jpg" width=200px /></a>
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Gel 2 of 2.  Lane 1: ladder; lanes 2-4: PCR product using VF2 and VR of plasmid containing <i>esp</i> ligated with <i>rsaA</i> C-terminal that had been digested 20110606; lane 5: PCR product of plasmid containing <i>esp</i>; lanes 6-8: PCR product using VF2 and VR of plasmid containing <i>esp</i> ligated with <i>rsaA</i> C-terminal PCR product from 20110605.  None of these show successful ligation.
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<a name="20110614_Promoters" href="https://2011.igem.org/File:20110614_Promoters.jpg"><img alt="Gel of Caulobacter promoters Pxyl and PrsaA" src="https://static.igem.org/mediawiki/2011/7/76/20110614_Promoters.jpg" width=200px /></a>
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Gel of PCR of <i>Caulobacter</i> promoters Pxyl (inducible) and PrsaA (constitutive). Lane 1: ladder; lane 2: PrsaA; Lane 3: Pxyl. Bands appear, but are hazy and spread out due to the small size of DNA fragments.
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<a name="20110616_promotorGel" href="https://2011.igem.org/File:20110616_promotorGel.jpg"><img alt="Gel of PCR products of PrsaA and Pxyl" src="https://static.igem.org/mediawiki/2011/9/9e/20110616_promotorGel.jpg" width=200px /></a>
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PCR products of <i>Caulobacter</i> promoters PrsaA and Pxyl.  Lane 1: ladder; lane 2: PrsaA; lane 3: Pxyl.
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<a name="20110616_promoterPostCleanup" href="https://2011.igem.org/File:20110616_promoterPostCleanup.jpg"><img alt="Gel showing promoter fragments after DNA clean up" src="https://static.igem.org/mediawiki/2011/2/2e/20110616_promoterPostCleanup.jpg" width=200px /></a>
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Gel confirming that clean up did not remove DNA fragments. Lane 1: ladder; lane 2: PrsaA; lane 3: Pxyl.
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<a name="20110617_Pro-esp-stop-rsaA" href="https://2011.igem.org/File:20110617_Pro-esp-stop-rsaA.jpg"><img alt="Gel of PCR products of transformations of insertion of Biobrick promoter BBa_K081005 into pSB1C3 containing esp and rsaA C-terminal" src="https://static.igem.org/mediawiki/2011/d/d6/20110617_Pro-esp-stop-rsaA.jpg" width=200px /></a>
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Gel of PCR products of transformations of insertion of Biobrick promoter BBa_K081005 into pSB1C3 containing esp and rsaA C-terminal. Lane 1: ladder; lanes 2-4 and 6-8: PCR product of transformation survivors; lane 5: control DNA of <i>esp</i> + <i>rsaA</i> C-terminal. Only lane 2 shows something that may be success, but as the fragment for the promoter is so small, it is difficult to confirm based solely on gel electrophoresis.
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<a name="20110617_rsaA%26esp" href="https://2011.igem.org/File:20110617_rsaA%26esp.jpg"><img alt="Gel of PCR products of esp and rsaA C-terminal using new primers." src="https://static.igem.org/mediawiki/2011/d/dd/20110617_rsaA%26esp.jpg" width=200px /></a>
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Gel of PCR product of <i>esp</i> and <i>rsaA</i> C-terminal using new primers. Lane 1: ladder; lanes 2,3: PCR product of <i>rsaA</i> from chromosomal <i>Caulobacter</i> DNA; lane 4: PCR product of <i>esp</i> using new primers from chromosomal <i>S. epidermidis</i> DNA.
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Latest revision as of 15:55, 25 July 2011

Grinnell Menubar

Gel Pics

Our first gel

We collected more gel data than we could fit on one page. Please go on to one of the following pages:

Retrieved from "http://2011.igem.org/Team:Grinnell/Notebook/Gels"