Team:KIT-Kyoto/Project

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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|align="center"|[[Team:KIT-Kyoto | Team Example]]
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<!--- The Mission, Experiments --->
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!align="left"|[[Team:KIT-Kyoto|Home]] > [[Team:KIT-Kyoto/Project|Project]]
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!align="right"|Language：[[Team:KIT-Kyoto/Project|English]]/[[Team:KIT-Kyoto/Projectjapanese|Japanese]]
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!align="center"|[[Team:KIT-Kyoto|Home]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=KIT-Kyoto Official Team Profile]
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!align="center"|[[Team:KIT-Kyoto/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:KIT-Kyoto/Modeling|Modeling]]
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<div id=NAKAMI>
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===Discription===
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:<span style="font-size:13pt;color:#000000;background-color:transparent;">L</span><span style="color:#000000;">eukemia is a serious disease and its remedy has been never found yet. People are afraid of it not only because it is incurable but also it is unpredictable what will happen and when it will happen. Patients have to put up with both of them.</span>
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:<span style="color:#000000;">Here we propose the convenient testing kit for leukemia which makes its symptom visible. To accomplish this we are developing leukemia disease models in ''Drosophila''. The usefulness of this kit will be evaluated by characterizing the ''Drosophila'' model. This kit may provide a guideline to overcome fear and pain which have not been immeasurable and relieve the fear for leukemia.</span>
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===Abstract===
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<span style="font-size:15pt;color:#000000;background-color:transparent;">Mr.D -who will cure leukemia-</span>
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<BR>
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:<span style="font-size:13pt;color:#000000;background-color:transparent;">O</span><span style="color:#000000;">ur team, KIT-Kyoto challenges making a leukemia disease model in ''Drosophila melanogaster''. ''Drosophila'' is being used as many hereditary disease model because over 70% of known human disease genes have similarities to Drosophila genes.</span>
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:<span style="color:#000000;">This year, we focus on a leukemia in which the etiology and the therapy have not been established. We therefore decided to make a leukemia disease model in ''Drosophila''. We insert human leukemia genes into ''Drosophila'' genome and also fuse a green fluorescent protein(GFP) with a leukemic protein to monitor its expression in ''E. coli'' or ''Drosophila''. We expect that establishing a leukemia disease model in ''Drosophila'' will be a first step to determine the etiology and to establish the method of therapy in the future.</span>
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== '''The fireworks using AHL as a transmitter.''' ==
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=== Results ===
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:<span style="font-size:13pt;color:#000000;background-color:transparent;">W</span><span style="color:#000000;">e have constructed a Biobrick part, BBa_K579000. This part is useful to make GFP-fusion with various proteins to monitor their expressions. Functional assays to monitor GFP expression in'' E. coli'' and ''Drosophila'' are ongoing. </span>
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:<span style="color:#000000;">We have succeeded to amplify API2-MALT1 cDNA and DIAP2 cDNA. Molecular cloning of these amplified DNA fragments into the P-element plasmid, pUAST-Flag are still underway. After construction of these plasmids, we are going to microinject them into ''Drosophila'' embryos to make transgenic flies.</span>
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The bacteria communicate with each other in the same way as people do so using some languages.This was called Quorum Sensing, and we paid our attention to this Quorum Sensing.In Quorum Sensing, the bacteria use the chemical substance called "autoinducer" in substitution for words used by a human being.The bacteria confirm how much density the same type of bacteria exists by around oneself with autoinducers.When they sense the density is high, the bacteria take action all at once. We plan to produce bioart using Quorum Sensing as materials this time.E. coli uses a chemical substance called "N-Acyl-Homoserin-Lacton(AHL)" as autoinducers.We thought whether we could reproduce fireworks by bioart using AHL as a communication factor.We use the GFP to express flower firelight.The GFP,which is green fluorescence protein discovered in Aequorea victoria, has the property of forming a chromophoric group (chemical structure to develop a color) alone. In short, fireworks will be launched and turned off by the following methods.<BR>① GPF is expressed in E.coli using AHL as a communication factor<BR>② Turn off the expressed GPF like a chain reaction.
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 03:23, 6 October 2011




Home Team Project Parts Notebook Safety Human Practice Attributions


Home > Project Language：English/Japanese

Discription

Leukemia is a serious disease and its remedy has been never found yet. People are afraid of it not only because it is incurable but also it is unpredictable what will happen and when it will happen. Patients have to put up with both of them.
Here we propose the convenient testing kit for leukemia which makes its symptom visible. To accomplish this we are developing leukemia disease models in Drosophila. The usefulness of this kit will be evaluated by characterizing the Drosophila model. This kit may provide a guideline to overcome fear and pain which have not been immeasurable and relieve the fear for leukemia.

Abstract

Mr.D -who will cure leukemia-

Our team, KIT-Kyoto challenges making a leukemia disease model in Drosophila melanogaster. Drosophila is being used as many hereditary disease model because over 70% of known human disease genes have similarities to Drosophila genes.
This year, we focus on a leukemia in which the etiology and the therapy have not been established. We therefore decided to make a leukemia disease model in Drosophila. We insert human leukemia genes into Drosophila genome and also fuse a green fluorescent protein(GFP) with a leukemic protein to monitor its expression in E. coli or Drosophila. We expect that establishing a leukemia disease model in Drosophila will be a first step to determine the etiology and to establish the method of therapy in the future.

Results

We have constructed a Biobrick part, BBa_K579000. This part is useful to make GFP-fusion with various proteins to monitor their expressions. Functional assays to monitor GFP expression in E. coli and Drosophila are ongoing.
We have succeeded to amplify API2-MALT1 cDNA and DIAP2 cDNA. Molecular cloning of these amplified DNA fragments into the P-element plasmid, pUAST-Flag are still underway. After construction of these plasmids, we are going to microinject them into Drosophila embryos to make transgenic flies.
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