Team:uOttawa

From 2011.igem.org

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Latest revision as of 07:52, 27 October 2011

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Saccharomyces cerevisiae (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in S. cerevisiae. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in S. cerevisiae. Finally, we succeeded in developing BrickMason Assembly, a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.

Team

This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.

BrickMason Assembly

Here we describe a convenient, efficient and cost-effective assembly method known as BrickMason assembly. Click on the image above to check out our animation and see how it works!

Results

Here we successfully demonstrate that BrickMason assembly can be used to assemble 6 brick constructs in just one days work. In addition, we were able to characterize 3 new BioBricks.

Parts Submitted

We submitted 8 new BioBricks to the registry this year.

Gears of Evolution

The uOttawa team has designed a 10 level game intended to teach and inform high school students about synthetic biology. This game is fully playable on our wiki, the iGEM community page and the Openwetware site Community Bricks. Click on the image above to play it and please give us feedback!

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-<p>Yeast has become a model organism due to its homology with mammalian organisms and its ease of genetic manipulation. For this reason, we believe it is important to develop tools that improve our ability to use yeast as a standard BioBrick organism. To this end, we aim to submit several BioBricks optimized to work in the simple eukaryote S. cerevisiae as well as build a novel yeast chassis that is capable of the robust characterization of these Biobricks. In addition, we are developing a new assembly protocol that will greatly improve upon existing BioBrick assembly protocols and allow for rapid integration into S. cerevisiae.</p>
+<p> <i>Saccharomyces cerevisiae</i> (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in <i>S. cerevisiae</i>. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in <i>S. cerevisiae</i>. Finally, we succeeded in developing BrickMason Assembly, a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/8/88/Team_uo.png') no-repeat !important;"><span>Team</span></h2></html><p>This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.</p>
+				<html><a href="/Team:uOttawa/Team"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/8/88/Team_uo.png') no-repeat !important;"><span>Team</span></h2></a></html><p>This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/b/b9/Project_uo.png') no-repeat !important;"><span>Project</span></h2></html><p>Characterization and standardizing of biological parts is a continuing problem for synthetic biologists. This year we decided to focus on developing a more efficient assembly method as well as optimizing characterization of BioBricks in S.cerevisiae.</p>
+				<html><a href="/Team:uOttawa/NewAssemblyProtocol"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/c/cd/BM3.png') no-repeat !important;"><span>BrickMason Assembly</span></h2></a></html><p>Here we describe a convenient, efficient and cost-effective assembly method known as BrickMason assembly. Click on the image above to check out our animation and see how it works!</p>
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 			<div id="s3" class="span-6 last section">
-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/5/51/Results_uo.png') no-repeat !important;"><span>Results</span></h2></html><p>We were able to successfully create a new assembly method as well as characterize transcription factors.</p>
+				<html><a href="/Team:uOttawa/Results"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/5/51/Results_uo.png') no-repeat !important;"><span>Results</span></h2></a></html><p>Here we successfully demonstrate that BrickMason assembly can be used to assemble 6 brick constructs in just one days work. In addition, we were able to characterize 3 new BioBricks.</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/2/2d/Parts_uo.png') no-repeat !important;"><span>Parts Submitted</span></h2></html><p>We submitted 7 new biobricks to the registry this year.</p>
+				<html><a target="_blank" href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=uOttawa"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/2/2d/Parts_uo.png') no-repeat !important;"><span>Parts Submitted</span></h2></a></html><p>We submitted 8 new BioBricks to the registry this year.</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/0/0d/Human_uo.png') no-repeat !important;"><span>Human Practices</span></h2></html><p>In order for the field synthetic biology to grow, more youth outreach is needed. The uOttawa team created a game to teach simple synbio theory and has arranged highschool tutorials for the fall. Also check out our essay.</p>
+				<html><a href="/Team:uOttawa/Game"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/0/0d/Human_uo.png') no-repeat !important;"><span>Gears of Evolution</span></h2></a></html><p>The uOttawa team has designed a 10 level game intended to teach and inform high school students about synthetic biology. This game is fully playable on our wiki, the iGEM community page and the Openwetware site Community Bricks. Click on the image above to play it and please give us feedback!</p>
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+			<div id="s6" class="span-6 section last">
+				<html><a href="/Team:uOttawa/Sponsors"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/1/1f/Sponsors_uo.png') no-repeat !important;"><span>Sponsors</span></h2></a></html><p>The uOttawa team would like to thank our sponsors for their generous support this year. Our sponsors graciously supplied us with lab equipment, reagents and funds necessary for the team to compete.</p>
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