"
Team:UC Davis/Project
From 2011.igem.org
(Difference between revisions)
Aheuckroth (Talk | contribs) |
|||
| (11 intermediate revisions not shown) | |||
| Line 18: | Line 18: | ||
<h1>Overview</h1> | <h1>Overview</h1> | ||
<div class="floatbox3"> | <div class="floatbox3"> | ||
| - | We set out to develop a | + | We set out to develop a quick, easy process for the expansion of basic parts into a part families. Our method employs a suped-up <a href="https://2011.igem.org/Team:UC_Davis/Protocols#ER-PCR">mutagenic PCR protocol</a> that uses standard VF2 and VR primers and materials most iGEM teams already have on hand. We chose to prototype this process by creating a part family from the LacI promoter R0010, and to mutate GFP to visually assess our ability to create functional protein mutants.<br><br> |
| - | As of | + | As of November 2011, we have a functioning part family generation process and seven <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">well-characterized</a> LacI promoter mutants and eight GFP mutants (two of which have been lovingly named Orange-Mutated Green Fluorescent Protein or [OMGfp] 1 and 2) which await further characterization.<br><br> |
| + | |||
| + | We have also begun the process of generating part families from the TetR (R0040) and Lambda c1 (R0051) promoters, which are currently being selected for screening. | ||
</div> | </div> | ||
| Line 29: | Line 31: | ||
<div class="floatboxright"> | <div class="floatboxright"> | ||
| - | <h2> | + | <h2>Make a Part Family</h2> |
| - | Want to make your own mutant library? Curious as to how we assayed our promoter mutants, or how we selected variants that had well-spaced activity levels? Read about our library generation process <a href="https://2011.igem.org/Team:UC_Davis/ | + | Want to make your own mutant library? Curious as to how we assayed our promoter mutants, or how we selected variants that had well-spaced activity levels? Read about our library generation process <a href="https://2011.igem.org/Team:UC_Davis/PartFamilies">here</a>. <br> |
</div> | </div> | ||
<div class="floatboxleft"> | <div class="floatboxleft"> | ||
<h2>Promoter Mutants</h2> | <h2>Promoter Mutants</h2> | ||
| - | We chose repressible promoters because they are useful in designing complex circuits and are relatively easy to screen for changes in activity level. You can view detailed information on our <a href="https://2011.igem.org/Team:UC_Davis/LacI">LacI</a>, <a href="https://2011.igem.org/Team:UC_Davis/TetR">TetR</a> and <a href="https://2011.igem.org/Team:UC_Davis/ | + | We chose to mutate repressible promoters because they are useful in designing complex circuits and are relatively easy to screen for changes in activity level. You can view detailed information on our <a href="https://2011.igem.org/Team:UC_Davis/PromoterFamilies#LacI">LacI</a>, <a href="https://2011.igem.org/Team:UC_Davis/PromoterFamilies#TetR">TetR</a> and <a href="https://2011.igem.org/Team:UC_Davis/PromoterFamilies#c1">λ cI</a> part families on their respective pages.<br><br> |
</div> | </div> | ||
Latest revision as of 20:15, 23 October 2011
Our Sponsors
Start a Family
Got a favorite BioBrick? Check our our process for expanding basic parts into part families.Criteria
View our judging criteria for iGEM 2011 here.
Overview
We set out to develop a quick, easy process for the expansion of basic parts into a part families. Our method employs a suped-up mutagenic PCR protocol that uses standard VF2 and VR primers and materials most iGEM teams already have on hand. We chose to prototype this process by creating a part family from the LacI promoter R0010, and to mutate GFP to visually assess our ability to create functional protein mutants.
As of November 2011, we have a functioning part family generation process and seven well-characterized LacI promoter mutants and eight GFP mutants (two of which have been lovingly named Orange-Mutated Green Fluorescent Protein or [OMGfp] 1 and 2) which await further characterization.
We have also begun the process of generating part families from the TetR (R0040) and Lambda c1 (R0051) promoters, which are currently being selected for screening.
As of November 2011, we have a functioning part family generation process and seven well-characterized LacI promoter mutants and eight GFP mutants (two of which have been lovingly named Orange-Mutated Green Fluorescent Protein or [OMGfp] 1 and 2) which await further characterization.
We have also begun the process of generating part families from the TetR (R0040) and Lambda c1 (R0051) promoters, which are currently being selected for screening.
