Team:Arizona State/Templates/E coli contingency/table 4

From 2011.igem.org

(Difference between revisions)
 
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! style="background: #ffdead;" | Initial Plasmid
! style="background: #ffdead;" | Initial Plasmid
! style="background: #ffdead;" | Introduced Plasmid
! style="background: #ffdead;" | Introduced Plasmid
 +
! style="background: #ffdead;" | Antibiotic selection
! style="background: #ffdead;" | DNA Quantity Used (ng)
! style="background: #ffdead;" | DNA Quantity Used (ng)
! style="background: #ffdead;" | # of Colonies
! style="background: #ffdead;" | # of Colonies
! style="background: #ffdead;" | Weighted Colony Count**
! style="background: #ffdead;" | Weighted Colony Count**
|-
|-
-
| MG1655 || GFP Construct in pRSF || Leader in pIDT || 140 || 21 || 138.742
+
| MG1655 || GFP Construct in pRSF || Leader in pIDT || Amp || 140 || 21 || 138.742
|-
|-
-
| MG1655 || GFP Construct in pRSF || Leader+Seq1 in pIDT || 147 || 31 || 204.810
+
| MG1655 || GFP Construct in pRSF || Leader+Seq1 in pIDT || Amp || 147 || 31 || 204.810
|-
|-
-
| MG1655 || Leader in pIDT || GFP Construct in pRSF || 143.4 || 23 || 117.635
+
| MG1655 || Leader in pIDT || GFP Construct in pRSF || Kan || 143.4 || 23 || 117.635
|-
|-
-
| MG1655 || Leader+Seq1 in pIDT || GFP Construct in pRSF || 143.4 || 1000 || 3909.508
+
| MG1655 || Leader+Seq1 in pIDT || GFP Construct in pRSF || Kan || 143.4 || 1000 || 3909.508
|-
|-
-
| BL21 || GFP Construct in pRSF || Leader in pIDT || 140 || 0 || 0.000
+
| BL21 || GFP Construct in pRSF || Leader in pIDT || Amp || 140 || 0 || 0.000
|-
|-
-
| BL21 || GFP Construct in pRSF || Leader+Seq1 in pIDT || 147 || 2 || 11.574
+
| BL21 || GFP Construct in pRSF || Leader+Seq1 in pIDT || Amp || 147 || 2 || 11.574
|-
|-
-
| BL21 || Leader in pIDT || GFP Construct in pRSF || 143.4 || 14 || 60.512
+
| BL21 || Leader in pIDT || GFP Construct in pRSF || Kan || 143.4 || 14 || 60.512
|-
|-
-
| BL21 || Leader+Seq1 in pIDT || GFP Construct in pRSF || 143.4 || 9 || 35.186
+
| BL21 || Leader+Seq1 in pIDT || GFP Construct in pRSF || Kan || 143.4 || 9 || 35.186
|}
|}
<small>''**Weighted Colony Count = (# of Colonies)/(mean OD600 of competency prep)''</small>
<small>''**Weighted Colony Count = (# of Colonies)/(mean OD600 of competency prep)''</small>

Latest revision as of 13:33, 28 September 2011

Transformation Efficiency Results
E. Coli Strain Initial Plasmid Introduced Plasmid Antibiotic selection DNA Quantity Used (ng) # of Colonies Weighted Colony Count**
MG1655 GFP Construct in pRSF Leader in pIDT Amp 140 21 138.742
MG1655 GFP Construct in pRSF Leader+Seq1 in pIDT Amp 147 31 204.810
MG1655 Leader in pIDT GFP Construct in pRSF Kan 143.4 23 117.635
MG1655 Leader+Seq1 in pIDT GFP Construct in pRSF Kan 143.4 1000 3909.508
BL21 GFP Construct in pRSF Leader in pIDT Amp 140 0 0.000
BL21 GFP Construct in pRSF Leader+Seq1 in pIDT Amp 147 2 11.574
BL21 Leader in pIDT GFP Construct in pRSF Kan 143.4 14 60.512
BL21 Leader+Seq1 in pIDT GFP Construct in pRSF Kan 143.4 9 35.186

**Weighted Colony Count = (# of Colonies)/(mean OD600 of competency prep)

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