Team:ITESM Mexico/Extras

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{{Team:ITESM_Mexico/Top}}
{{Team:ITESM_Mexico/Top}}
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Some aditional stuff.
 
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[[File:Giant_microbe.png|center]]
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<html><h1 class="myowntitle">Extras</h1> </html>
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-----
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Home made quick saliva DNA extraction protocol:
 
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• Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
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::'''Some aditional stuff.'''
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[[File:Giant_microbe.png|center]]
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==='''Home made quick saliva DNA extraction protocol:'''===
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• Prepare a solution with soap and water in a beaker
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::#Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
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::#Prepare a solution with soap and water in a beaker
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::#Add the soap solution to the last rinse
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::#Add frozen (0°C) etanol  to the last rinse
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::#Observe how a DNA thread forms, take it and place it in an eppendorf tube.
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• Add the soap solution to the last rinse
 
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• Add frozen (0°C) etanol  to the last rinse
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===Abbreviations and glossary:===
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• Observe how a DNA thread forms, take it and place it in an eppendorf tube.
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::*'''Biobrickoatl:'''It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
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::*'''Competent cells:''' Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
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::*'''CcaS-EnvZ: '''Is the green light receptor that induces the expresión of phycobilisome linker protein.
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::*'''CFP:''' Cyan Fluorescent Protein
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::*'''Crst:''' Lock for the PbadST (strong, for low concentrations) promoter
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::*'''CrWk:''' Lock for the Pbad WK (weak, for high concentration) promoter
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::*'''GFP:''' Green Fluorescent Protein
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::*'''ho1:''' Heme oxigenase 1
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::*'''iTaST:''' Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
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::*'''pBADST:''' Strong arabinose promoter for low concentration detection.
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::*'''pBADWk:''' Weak arabinose promoter for high concentration detection.
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::*'''Pcy4A:''' Phycocyanobilin ferredoxin oxidoreductase.
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::*'''RBS:''' Ribosome binding site
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::*'''TaST:''' Key for the CrST lock
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::*'''TaWk:''' Key for the CrWk lock
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::*'''Term:''' Terminator, not the robot (genetic terminator).

Latest revision as of 03:28, 29 September 2011

ITESM MÉXICO

SensE.coli

Igem Itesm

Extras


Some aditional stuff.
Giant microbe.png

Home made quick saliva DNA extraction protocol:

  1. Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
  2. Prepare a solution with soap and water in a beaker
  3. Add the soap solution to the last rinse
  4. Add frozen (0°C) etanol to the last rinse
  5. Observe how a DNA thread forms, take it and place it in an eppendorf tube.


Abbreviations and glossary:

  • Biobrickoatl:It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
  • Competent cells: Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
  • CcaS-EnvZ: Is the green light receptor that induces the expresión of phycobilisome linker protein.
  • CFP: Cyan Fluorescent Protein
  • Crst: Lock for the PbadST (strong, for low concentrations) promoter
  • CrWk: Lock for the Pbad WK (weak, for high concentration) promoter
  • GFP: Green Fluorescent Protein
  • ho1: Heme oxigenase 1
  • iTaST: Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
  • pBADST: Strong arabinose promoter for low concentration detection.
  • pBADWk: Weak arabinose promoter for high concentration detection.
  • Pcy4A: Phycocyanobilin ferredoxin oxidoreductase.
  • RBS: Ribosome binding site
  • TaST: Key for the CrST lock
  • TaWk: Key for the CrWk lock
  • Term: Terminator, not the robot (genetic terminator).
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