Team:Kyoto

From 2011.igem.org

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==<span class="red_title">Summary</span>==
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[[File:京都スライドショウ.jpg]]
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We created new E.coli which hunts and eats insects.
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== Summary ==
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As such we named it Carnivorous E.coli.
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We create new E.coli which hunt and eat insects.
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Carnivorous E.coli emits light when it is hungry. This will attract insects to it.
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it is named Carnivorous E.coli.
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Then, it secretes a viscous substance to capture the insects.
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Carnivorous E.coli emits light when hungry, and insects come near it attracted by the light.
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The captured insects are digested by the chitinase in the secretion.
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Then, it secretes viscous material to catch the insects.
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Long story short, our Carnivorous E.coli can hunt!
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The insects caught are solved by protease and chitinase that Carnivorous E.coli secretes also.
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<!-- https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png -->
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So, Carnivorous E.coli can hunt!
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== <html><a href="https://2011.igem.org/Team:Kyoto/Hunger">Hunger</a></html> ==
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<div id="project_panel">
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It is a burden for the E.coli to emit light.
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== <html><a href="/Team:Kyoto/Hunger"><span class="red_title">Team Hunger</span></a></html> ==
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This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough.
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<html><div id="hunger"><a href="/Team:Kyoto/Hunger"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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Carnivorous E.coli attracts insects by emitting light, but it is a burden for ''E.coli''.
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To reduce this burden, we use nitrogen regulatory proteins, NtrB and NtrC. They activate &sigma;<sup>54</sup> promoter when the supply of nitrogen is not enough.
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== <html><a href="https://2011.igem.org/Team:Kyoto/Luminescence">Luminescence</a></html> ==
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== <html><a href="/Team:Kyoto/Capture"><span class="green_title">Team Capture</span></a></html> ==
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<html><div id="capture"><a href="/Team:Kyoto/Capture"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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There are many methods to attract bugs, for instance, using pheromone, smell, and light.
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In this study, we focused on light as a method to lure insects.
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Carnivorous E.coli will emit light and attracts bugs like glowworms by using Bioluciferase from <html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>.
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<!--
There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone.
There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone.
Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from
Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from
<html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>.
<html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>.
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-->
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== <html><a href="/Team:Kyoto/Digestion"><span class="blue_title">Team Digestion</span></a></html> ==
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<html><div id="digestion"><a href="/Team:Kyoto/Digestion"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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An insect body is covered with a hard integument containing mainly chitin. To decompose the integument, we used ChiA gene, which encode chitinase. In order to measure the chitinase activity of the culture supernatant, we evaluated the effects of the medium and cell growth.
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== <html><a href="https://2011.igem.org/Team:Kyoto/Digestion">Digestion</a></html> ==
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#hunger, #capture, #digestion {
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<!-- end project_panel -->
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In this part, we aimed to create ''E.coli'' which digest bugs and survive under the nitrogen-poor condition. So, we decided to use SAM-P20 and ChiA genes, which encode protease and chitinase. In order to measure the activity of each enzyme correctly, we examined assay methods first.
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<div id="under_box">
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==Achievement==
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==<span class="red_title">Achievement</span>==
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<ul>
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  <li>We confirmed that the wavelength of light emitted by E.coli is suitable to lure drosophilas.</li>
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  <li>We constructed new biobrick which produces chitinase.</li>
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  <li>We constructed new biobrick that is controlled by the concentration of glutamine.</li>
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  <li>We established new method for measurement of promoter activity with RPU.</li>
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  <li>We held <html><a href="https://2011.igem.org/Summer_events/2011_JAPAN_Meetup">the Japanese iGEM meeting</a></html> at Kyoto University.
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</ul>
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==<html><a href="https://2011.igem.org/Team:Kyoto/HumanPractice">Human Practice</a></html>==
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==<html><a href="https://2011.igem.org/Team:Kyoto/HumanPractice"><span class="green_title">Human Practice</span></a></html>==
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As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However, this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities.
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==Reference==
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==<span class="yellow_title">Sponsors</span>==
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[[File:京大ロゴ.jpg | link=http://www.kyoto-u.ac.jp/|]]
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[[File:CosmoBio.jpg | link=http://www.cosmobio.co.jp |]]
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Latest revision as of 03:58, 6 October 2011

Contents

Summary

We created new E.coli which hunts and eats insects. As such we named it Carnivorous E.coli. Carnivorous E.coli emits light when it is hungry. This will attract insects to it. Then, it secretes a viscous substance to capture the insects. The captured insects are digested by the chitinase in the secretion. Long story short, our Carnivorous E.coli can hunt!

Team Hunger

Carnivorous E.coli attracts insects by emitting light, but it is a burden for E.coli. To reduce this burden, we use nitrogen regulatory proteins, NtrB and NtrC. They activate σ54 promoter when the supply of nitrogen is not enough.

Team Capture

There are many methods to attract bugs, for instance, using pheromone, smell, and light. In this study, we focused on light as a method to lure insects. Carnivorous E.coli will emit light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.

Team Digestion

An insect body is covered with a hard integument containing mainly chitin. To decompose the integument, we used ChiA gene, which encode chitinase. In order to measure the chitinase activity of the culture supernatant, we evaluated the effects of the medium and cell growth.

Achievement

  • We confirmed that the wavelength of light emitted by E.coli is suitable to lure drosophilas.
  • We constructed new biobrick which produces chitinase.
  • We constructed new biobrick that is controlled by the concentration of glutamine.
  • We established new method for measurement of promoter activity with RPU.
  • We held the Japanese iGEM meeting at Kyoto University.

Human Practice

As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However, this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities.

Sponsors

京大ロゴ.jpg CosmoBio.jpg

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