Team:Arizona State/Notebook/September

From 2011.igem.org

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Line 6: Line 6:
:* Listeria CRISPR only uses cas9 tracr gene
:* Listeria CRISPR only uses cas9 tracr gene
:* Project requires:
:* Project requires:
-
::* cas9
+
::* Cas9
::* Duet plasmid
::* Duet plasmid
::* Listeria T7 RSR
::* Listeria T7 RSR
Line 16: Line 16:
:* sample appears slightly higher than expected
:* sample appears slightly higher than expected
::* some distortion and low resolution
::* some distortion and low resolution
-
== Sunday, September 4 ==
+
== Sunday, September 4 to Friday, September 9 ==
-
== Monday, September 5 ==
+
* Cas9 PCR products restricted overnight
-
== Tuesday, September 6 ==
+
-
== Wednesday, September 7 ==
+
-
== Thursday, September 8 ==
+
-
== Friday, September 9 ==
+
-
* cas9 PCR products restricted overnight
+
== Saturday, September 10 ==
== Saturday, September 10 ==
* 9/10 restriction products dephosphorylated for 2 hours
* 9/10 restriction products dephosphorylated for 2 hours
* Overnight ligation of dephosphorylated restriction products
* Overnight ligation of dephosphorylated restriction products
-
== Sunday, September 11 ==
+
== Sunday, September 11 to Saturday, September 17 ==
* 9/8 restriction - 9/10 ligation products transformed into NEB cells  
* 9/8 restriction - 9/10 ligation products transformed into NEB cells  
-
== Monday, September 12 ==
+
== Sunday, September 18 to Saturday, September 24 ==
-
== Tuesday, September 13 ==
+
*Attempt to assemble CMR-RSR using overlapping oligos
-
*
+
*Began preparation of pSB1C3 plasmid for BioBrick preparation
-
== Wednesday, September 14 ==
+
== Sunday, September 25 to Wednesday, September 28 ==
-
== Thursday, September 15 ==
+
*Finalized BioBrick preparation for intended BioBrick part submission
-
== Friday, September 16 ==
+
*Restrict, ligate, and transform CMR and pRSF. 9/25 Send sample to sequence. 9/27 Sequencing error. They will re-run.
-
== Saturday, September 17 ==
+
*Wiki parties all week in the lab!
-
== Sunday, September 18 ==
+
*Submitted BioBricks!
-
== Monday, September 19 ==
+
-
== Tuesday, September 20 ==
+
-
== Wednesday, September 21 ==
+
-
== Thursday, September 22 ==
+
-
== Friday, September 23 ==
+
-
== Saturday, September 24 ==
+
-
== Sunday, September 25 ==
+
-
== Monday, September 26 ==
+
-
== Tuesday, September 27 ==
+
-
== Wednesday, September 28 ==
+
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Latest revision as of 03:09, 29 September 2011


Notebook: September


ASU Logo.png
 

Thursday, September 1

  • Started to develop Listeria Innocua project component.
  • Listeria CRISPR only uses cas9 tracr gene
  • Project requires:
  • Cas9
  • Duet plasmid
  • Listeria T7 RSR
  • Ligate all together in biobrick
  • Test with protospacer

Saturday, September 3

  • 3 PCR reactions using LI as template
  • Samples visualized on gel
  • sample appears slightly higher than expected
  • some distortion and low resolution

Sunday, September 4 to Friday, September 9

  • Cas9 PCR products restricted overnight

Saturday, September 10

  • 9/10 restriction products dephosphorylated for 2 hours
  • Overnight ligation of dephosphorylated restriction products

Sunday, September 11 to Saturday, September 17

  • 9/8 restriction - 9/10 ligation products transformed into NEB cells

Sunday, September 18 to Saturday, September 24

  • Attempt to assemble CMR-RSR using overlapping oligos
  • Began preparation of pSB1C3 plasmid for BioBrick preparation

Sunday, September 25 to Wednesday, September 28

  • Finalized BioBrick preparation for intended BioBrick part submission
  • Restrict, ligate, and transform CMR and pRSF. 9/25 Send sample to sequence. 9/27 Sequencing error. They will re-run.
  • Wiki parties all week in the lab!
  • Submitted BioBricks!