Ampicillin stock

From 2011.igem.org

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Materials:
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'''Materials:'''
Ampicillin powder
Ampicillin powder
ddH2O
ddH2O
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sterile syringe
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syringe filter
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sterile 15ml centrifuge tube
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'''Procedure:'''
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1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (''we use 0.25g in 5 ml of H2O to make a 50mg/ml solution.'')
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2. Mix your antibiotic with your ddH2O
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3. Absorb your solution with the sterile syringe.
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4. Place the syringe filter onto the syringe.
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5. Filter your solution into the sterile 15ml centrifuge tube.
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[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''BACK''']
[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''BACK''']

Latest revision as of 20:35, 28 September 2011

Materials:

Ampicillin powder

ddH2O

sterile syringe

syringe filter

sterile 15ml centrifuge tube

Procedure:

1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (we use 0.25g in 5 ml of H2O to make a 50mg/ml solution.)

2. Mix your antibiotic with your ddH2O

3. Absorb your solution with the sterile syringe.

4. Place the syringe filter onto the syringe.

5. Filter your solution into the sterile 15ml centrifuge tube.


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