Team:USC/Safety
From 2011.igem.org
(Difference between revisions)
Kevinle1992 (Talk | contribs) |
|||
(2 intermediate revisions not shown) | |||
Line 3: | Line 3: | ||
<tr> | <tr> | ||
- | <td | + | <td colspan="3">[[File:USC_Banner.jpg]]</td> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td colspan=" | + | <td colspan="3"> |
<div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;"> | <div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;"> | ||
<ul> | <ul> | ||
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC|Home]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Team|Team]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Project|Project]]</span></span> |
- | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Notebook|Notebook]]</span></span> | |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Parts|Parts]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Safety|Safety]]</span></span> |
- | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Human Outreach|Human Outreach]]</span></span> | |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span> |
</ul> | </ul> | ||
+ | |||
</div> | </div> | ||
+ | |||
+ | |||
</td> | </td> | ||
</tr> | </tr> | ||
- | |||
<tr> | <tr> | ||
- | <td> | + | <td style="width:100px; vertical-align:top;"> |
- | < | + | |
- | = | + | <td style="width:724px;"> |
- | = | + | |
+ | <h1 style="font-family:Verdana;font-weight:700;">Biological Safety Concerns Regarding E. curi</h1> | ||
+ | |||
+ | [[File:humanpractice1.jpg|center]] | ||
+ | |||
+ | <h1 style="font-family:Verdana;font-weight:300;">Identifying Safety Issues in Our CRISPR Project</h1> | ||
<p>Working in a lab can be fun but also dangerous if safety for the student, society, and environment are not addressed. Therefore, the safety issues of these three important entities will be examined under the scope of risk assessment. To assess risk for a biological material many factors have to be considered, which include but are not limited to: the microbe's pathogenicity and ability to survive outside of the lab, public awareness of the biological agent, availability of a prophylaxis, environmental impact, and ability to monitor biological material that are potentially dangerous.</p> | <p>Working in a lab can be fun but also dangerous if safety for the student, society, and environment are not addressed. Therefore, the safety issues of these three important entities will be examined under the scope of risk assessment. To assess risk for a biological material many factors have to be considered, which include but are not limited to: the microbe's pathogenicity and ability to survive outside of the lab, public awareness of the biological agent, availability of a prophylaxis, environmental impact, and ability to monitor biological material that are potentially dangerous.</p> | ||
<p>We assess risk for the biological material involved in our project and its potential impact to the researchers, public society, and environment by taking into account two components of risk: probability and hazard. With probability, we ask ourselves:</p> | <p>We assess risk for the biological material involved in our project and its potential impact to the researchers, public society, and environment by taking into account two components of risk: probability and hazard. With probability, we ask ourselves:</p> | ||
Line 62: | Line 57: | ||
</ul> | </ul> | ||
<p>We assess biological safety risks with these questions in mind in an effort to make our project acceptably safe according to the standards set by organizations, such as the World Health Organization (WHO), the Convention on Biological Diversity, the National Institute of Health (NIH), American Biological Safety Association, Center for Disease Control, among other risk management groups, and conferences with an emphasis in synthetic biology and safety such as the SB5.0 conference. In addition, complying with these safety standards will make the project and its future directions more approachable for acceptance by the public society.</p> | <p>We assess biological safety risks with these questions in mind in an effort to make our project acceptably safe according to the standards set by organizations, such as the World Health Organization (WHO), the Convention on Biological Diversity, the National Institute of Health (NIH), American Biological Safety Association, Center for Disease Control, among other risk management groups, and conferences with an emphasis in synthetic biology and safety such as the SB5.0 conference. In addition, complying with these safety standards will make the project and its future directions more approachable for acceptance by the public society.</p> | ||
- | = | + | <h1 style="font-family:Verdana;font-weight:300;">Researcher Safety</h1> |
<p>Ensuring the safety of the USC iGEM lab and its researchers is critical to the success of the program. We have therefore taken initiative that each member understands and is aware of the following:</p> | <p>Ensuring the safety of the USC iGEM lab and its researchers is critical to the success of the program. We have therefore taken initiative that each member understands and is aware of the following:</p> | ||
<ul> | <ul> | ||
Line 84: | Line 79: | ||
</ul> | </ul> | ||
<p>To ensure the researchers of USC iGEM are acceptably safe from physical harm by biological material or chemical exposure, each team member attended a Safety Orientation Course conducted by USC's Career and Protective Services (CAPS). In addition, the protocols used in our project have been approved by the Institutional Biosafety Committee.</p> | <p>To ensure the researchers of USC iGEM are acceptably safe from physical harm by biological material or chemical exposure, each team member attended a Safety Orientation Course conducted by USC's Career and Protective Services (CAPS). In addition, the protocols used in our project have been approved by the Institutional Biosafety Committee.</p> | ||
- | + | <h1 style="font-family:Verdana;font-weight:300;">Public Safety</h1> | |
- | + | ||
<p>Although the E. coli strains we are using are non-pathogenic, we remain aware that our project can potentially be a concern for public safety. By addressing this issue early, we lower the risk of fear and mistrust that can be met by the scrutiny of the general public when introducing a new biotechnological tool to the marketplace and diminish the uncalculated possibility of causing sever physical and non-physical harm. Our approach to public safety can be categorized as follows:</p> | <p>Although the E. coli strains we are using are non-pathogenic, we remain aware that our project can potentially be a concern for public safety. By addressing this issue early, we lower the risk of fear and mistrust that can be met by the scrutiny of the general public when introducing a new biotechnological tool to the marketplace and diminish the uncalculated possibility of causing sever physical and non-physical harm. Our approach to public safety can be categorized as follows:</p> | ||
<ul> | <ul> | ||
Line 104: | Line 98: | ||
<p>These simple procedures are implemented to ensure that the public in our immediate surroundings is respected with our safety concerns for them.</p> | <p>These simple procedures are implemented to ensure that the public in our immediate surroundings is respected with our safety concerns for them.</p> | ||
<p>At first, it may seem difficult to judge the effect of our project on public safety on a national level. However, the CRISPR system in our model is designed to counteract potential spreading and resistance of harmful microbes. As such, our project may in fact increase public safety and health.</p> | <p>At first, it may seem difficult to judge the effect of our project on public safety on a national level. However, the CRISPR system in our model is designed to counteract potential spreading and resistance of harmful microbes. As such, our project may in fact increase public safety and health.</p> | ||
- | + | <h1 style="font-family:Verdana;font-weight:300;">Environmental Safety</h1> | |
- | + | ||
<p>The safety of the surrounding environment should not be compromised. The E. coli strain is non-pathogenic and equipment and reagents are disinfected with the proper chemicals when cleaning materials to avoid spreading and contamination.</p> | <p>The safety of the surrounding environment should not be compromised. The E. coli strain is non-pathogenic and equipment and reagents are disinfected with the proper chemicals when cleaning materials to avoid spreading and contamination.</p> | ||
Line 143: | Line 136: | ||
<p>We also spoke with the department about our specific project on CRISPR, and they saw no safety issues within our laboratory.</p> | <p>We also spoke with the department about our specific project on CRISPR, and they saw no safety issues within our laboratory.</p> | ||
- | = | + | <h1 style="font-family:Verdana;font-weight:500;">Thoughts on How to Improve Safety in iGEM</h1> |
<p>iGEM teams could test antibiotic sensitivity in all bacteria strains periodically, making sure that these strains will not survive an antibiotic that we expect the strains to be sensitive to. Parts, devices, and systems could be made even safer by having iGEM require mandatory laboratory safety check-ups during the summer as well as submit documentation of the safe practices employed.</p> | <p>iGEM teams could test antibiotic sensitivity in all bacteria strains periodically, making sure that these strains will not survive an antibiotic that we expect the strains to be sensitive to. Parts, devices, and systems could be made even safer by having iGEM require mandatory laboratory safety check-ups during the summer as well as submit documentation of the safe practices employed.</p> | ||
- | |||
- | |||
</table> | </table> |