Team:Tec-Monterrey/projectnotebook may/day20

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20/May/2011
20/May/2011
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• DNA digestión with SspI and NdeI + Buffer 3 at 37 C , 1 hr.
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• DNA digestion with SspI and NdeI + Buffer 3 at 37 C , 1 hr.
</br>
</br>
• Electrophoresis 60 V, 1.5 dr.
• Electrophoresis 60 V, 1.5 dr.
</br>
</br>
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• Pre- inocolum of virgin cells in 15-20 microliters of LB without antibiotic
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• Pre-inocolum of virgin cells in 15-20 microliters of LB without antibiotic
for calcium competent cells.
for calcium competent cells.
</br>
</br>

Latest revision as of 03:46, 18 October 2011

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20/May/2011
• DNA digestion with SspI and NdeI + Buffer 3 at 37 C , 1 hr.
• Electrophoresis 60 V, 1.5 dr.
• Pre-inocolum of virgin cells in 15-20 microliters of LB without antibiotic for calcium competent cells.
• Purification of DNA of the agarose gel.


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