Team:Calgary/Notebook/Protocols/Process20

From 2011.igem.org

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   <ul>
   <ul>
     <li> Overnight bacterial growth </li>
     <li> Overnight bacterial growth </li>
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     <li> screw captubes </li>
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     <li> Screw Cap Tubes </li>
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     <li> glycerol </li>
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     <li> Glycerol </li>
   </ul>
   </ul>
   <p> Protocol </p>
   <p> Protocol </p>
   <ol>
   <ol>
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     <li> Pipet 0.5mL of 50% glycerol into 3 1.5 screw cap tubes</li>
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     <li> Pipet 0.5mL of 50% glycerol into 3 1.5mL screw cap tubes</li>
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     <li> Add 0.5mL of overnight culture to each tube </li>
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     <li> Add 0.5mL of overnight culture to each tube. </li>
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     <li> Pipet up and down to gently mix</li>
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     <li> Pipet up and down to gently mix.</li>
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     <li> Flash freeze  in liquid N2 or dry ice/ethanol bath </li>
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     <li> Flash freeze  in liquid N2 or dry ice/ethanol bath. </li>
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     <li> Place in -80 C freezer when frozen </li>
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     <li> Place in -80°C freezer when frozen. </li>
   </ol>
   </ol>
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Latest revision as of 04:13, 29 September 2011


Preparing Glycerol Stocks

Reagents and Materials

  • Overnight bacterial growth
  • Screw Cap Tubes
  • Glycerol

Protocol

  1. Pipet 0.5mL of 50% glycerol into 3 1.5mL screw cap tubes
  2. Add 0.5mL of overnight culture to each tube.
  3. Pipet up and down to gently mix.
  4. Flash freeze in liquid N2 or dry ice/ethanol bath.
  5. Place in -80°C freezer when frozen.








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