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| - | <li><a class="head" href="#">Project Firefly</a>
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| - | <li><a href="/Team:Cambridge/Bioluminescence">Introduction</a></li>
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| - | <li><a href="/Team:Cambridge/Bioluminescence/Background_Firefly">Background</a></li>
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| - | <li><a href="/Team:Cambridge/Bioluminescence/Colour">Coloured outputs</a></li>
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| - | <li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">Introduction</a></li>
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| - | <li><a href="/Team:Cambridge/Bioluminescence/Background">Background</a></li>
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| - | <li><a href="/Team:Cambridge/Bioluminescence/G28">The LuxBrick</a></li>
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| - | <li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Modelling">Modelling</a></li>
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| - | <li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Characterisation">Characterisation</a></li>
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| - | <li><a href="/Team:Cambridge/Tools/Gibson">Gibthon</a></li>
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| - | <strong class="selflink">Making long term stocks</strong> | <a href="/Team:Cambridge/Protocols/Comp" title="Team:Cambridge/Protocols/Comp">Transformation</a> | <a href="http://microscopy.berkeley.edu/Resources/instruction/buffers.html" class="external text" title="http://microscopy.berkeley.edu/Resources/instruction/buffers.html" rel="nofollow">Buffer recipes</a> | <a href="/Team:Cambridge/Protocols/ColonyPCR" title="Team:Cambridge/Protocols/ColonyPCR">Colony PCR</a>
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| - | <a name="Making_long_term_stocks_in_glycerol"></a><h1><span class="editsection">[<a href="/wiki/index.php?title=Team:Cambridge/Protocols/Long&action=edit&section=1" title="Edit section: Making long term stocks in glycerol">edit</a>]</span> <span class="mw-headline">Making long term stocks in glycerol</span></h1>
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| - |
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| - | <p>(From <a href="http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria" class="external free" title="http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria" rel="nofollow">http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria</a>)
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| - | </p>
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| - | <a name="Materials"></a><h2><span class="editsection">[<a href="/wiki/index.php?title=Team:Cambridge/Protocols/Long&action=edit&section=2" title="Edit section: Materials">edit</a>]</span> <span class="mw-headline">Materials</span></h2>
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| - | <ul><li>40% glycerol solution
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| - | </li><li>Cryogenic vials
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| - | </li></ul>
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| - | <a name="Method"></a><h2><span class="editsection">[<a href="/wiki/index.php?title=Team:Cambridge/Protocols/Long&action=edit&section=3" title="Edit section: Method">edit</a>]</span> <span class="mw-headline">Method</span></h2>
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| - |
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| - | <ul><li>Add 1 ml of 40% glycerol in H<sub>2</sub>O to a cryogenic vial.
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| - | </li><li>Add 1 ml sample from the culture of bacteria to be stored.
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| - | </li><li>Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
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| - | <ul><li>Alternatively, pipet to mix.
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| - | </li></ul>
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| - | </li><li>Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
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| - | </li><li>On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
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| - | </li><li>Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.
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| - | </li></ul>
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| - | <a name="Notes"></a><h2><span class="editsection">[<a href="/wiki/index.php?title=Team:Cambridge/Protocols/Long&action=edit&section=4" title="Edit section: Notes">edit</a>]</span> <span class="mw-headline">Notes</span></h2>
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| - |
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| - | <ul><li>While it is possible to make a long term stock from cells in stationary phase, ideally your culture should be in logarithmic growth phase.
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