Team:Panama/Notebook/June2011

From 2011.igem.org

(Difference between revisions)
(→June 29, 2011)
 
(6 intermediate revisions not shown)
Line 4: Line 4:
We did a lot of work today like:  
We did a lot of work today like:  
 +
1. The miniprep of the strains containing the BioBrick rh1AB.  
1. The miniprep of the strains containing the BioBrick rh1AB.  
-
2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA.
+
 
-
3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium.
+
2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA.
-
4. Last attempt in seed the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol  (33 ug/ml).
+
-
5. We did all the necessary calculations for the antibiotics concentrations.
+
   
   
 +
3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium.
 +
 +
4. Last attempt to inoculate the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol  (33 ug/ml).
 +
 +
5. We did all the necessary calculations for the antibiotics concentrations.                                           
 +
                                   
 +
'''Rh1AB + psB1C3 Ectroforesis'''
 +
[[File:e29-6-11.jpg|300px|thumb|left|alt text]]
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
-
                 
+
1 and 8: Marker 100- 3000 pb. 2-7: rh1AB 2010 BioBrick
-
                                  '''Rh1AB + psB1C3 Ectroforesis'''
+
-
                                      [[File:e29-7-11.jpg]]
+

Latest revision as of 03:58, 27 September 2011