Team:Johns Hopkins/Data

From 2011.igem.org

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{{:Team:Johns_Hopkins/Templates/tpl1}}
{{:Team:Johns_Hopkins/Templates/tpl1}}
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__NOTOC__
__NOTOC__
======Data Page======
======Data Page======
===== Vitamin A Production =====
===== Vitamin A Production =====
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[[Image:beta-carotene datapage.jpg]]
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<center>[[Image:beta-carotene datapage.jpg|700px]]</center>
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===== Vitamin C Production =====
===== Vitamin C Production =====
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[[Image:L-ascorbate datapage.jpg]]
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<center>[[Image:L-ascorbate datapage.jpg|700px]]</center>
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===== Yeast Toolkit =====
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{| border="1" cellpadding="5" cellspacing="0" style="margin-left:40px;"
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===== Yeast Toolkit =====
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{| id="toolkittable" border="1" cellpadding="5" cellspacing="0" style="margin-left:auto;margin-right:auto;"
|- style="background-color:#EBEBE9;"
|- style="background-color:#EBEBE9;"
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|Favorite New Parts
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|[[Image:Pros.jpg|center|x100px]]
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|[[Image:Utrs.jpg|center|x100px]]
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|[[Image:Vectors.jpg|center|x100px]]
|-  
|-  
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530000 CrtYB]
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|
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|-
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530004 STM1p]<br>
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530001 CrtE]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530005 BAP2p]<br>
-
|-
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530007 PRY1p]<br>
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530002 CrtI]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530009 RPS2p]<br>
-
|-
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p]<br>
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530025 GDP-L-Gal Phosphorylase]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530010 HHO1p]<br>
-
|-
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530015 FCY2p]<br>
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530026 GDP-Man-3,5-Epimerase]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530016 RPS8Bp]
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|-
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530027 L-Gal-1-Phos-Phosphatase]
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-
|}
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|
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530006 BAP2u]<br>
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530014 KRE9u]<br>
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530017 FCY2u]<br>
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHO1u]<br>
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1u]
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{| border="1" cellpadding="5" cellspacing="0" style="margin-left:40px;"
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|
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|- style="background-color:#EBEBE9;"
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'''Integrating vectors'''<br>
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|Existing Parts Characterized
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530028 pRSBB403 (HIS3)]<br>
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|-
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530029 pRSBB404 (TRP1)]<br>
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J63006 Gal1p]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530030 pRSBB405 (LEU2)]<br>
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|-
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530031 pRSBB406 (URA3)]<br>
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'''CEN/ARS vectors'''<br>
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530032 pRSBB413 (HIS3)]<br>
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530033 pRSBB414 (TRP1)]<br>
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530034 pRSBB415 (LEU2)]<br>
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530035 pRSBB416 (URA3)]<br>
 +
'''2-Micron vectors'''<br>
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530036 pRSBB423 (HIS3)]<br>
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530037 pRSBB424 (TRP1)]<br>
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530038 pRSBB425 (LEU2)]<br>
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530039 pRSBB426 (URA3)]
|}
|}
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 +
====== Favorite New Parts ======
 +
 +
===Vitamin A Production===
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530000 CrtYB] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is a fusion, it is the full Phytoene Synthase enzyme spliced with a Lycopene B-Cyclase enzymatic domain. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530001 CrtE] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530002 CrtI] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene.
 +
 +
===Vitamin C Production===
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530025 VTC2] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of GDP-L-Galactose to L-Galactose-1-P.
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530026 GME] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose-1-P to L-Galactose.
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530027 VTC4] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose to L-Galactono-1,4-Lactone.
 +
 +
===Promoters===
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530003 KRE9p] Promoter used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p] Promoter used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
 +
 +
===Terminators===
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHO1utr] Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1utr] Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
 +
 +
===Vectors===
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530032 pRSBB413 HIS3 CEN/ARS Yeast Shuttle Vector] Used for the propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530039 pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector] Used for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.
 +
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=== Existing Parts Characterized ===
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_J63006 Gal1p]. This characterization is a work in progress. We will update the part's experience page after the regional jamboree. We are working with the British Columbia team on troubleshooting.
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======All Submitted Parts======
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<center><groupparts>iGEM011 Johns_Hopkins</groupparts></center>
<html></div>
<html></div>
<br/><br/>
<br/><br/>

Latest revision as of 19:44, 28 September 2011

VitaYeast - Johns Hopkins University, iGEM 2011

Data Page
Vitamin A Production
Beta-carotene datapage.jpg
Vitamin C Production
L-ascorbate datapage.jpg
Yeast Toolkit
Pros.jpg
Utrs.jpg
Vectors.jpg

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530004 STM1p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530005 BAP2p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530007 PRY1p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530009 RPS2p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530010 HHO1p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530015 FCY2p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530016 RPS8Bp]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530006 BAP2u]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530014 KRE9u]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530017 FCY2u]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHO1u]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1u]

Integrating vectors
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530028 pRSBB403 (HIS3)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530029 pRSBB404 (TRP1)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530030 pRSBB405 (LEU2)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530031 pRSBB406 (URA3)]
CEN/ARS vectors
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530032 pRSBB413 (HIS3)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530033 pRSBB414 (TRP1)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530034 pRSBB415 (LEU2)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530035 pRSBB416 (URA3)]
2-Micron vectors
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530036 pRSBB423 (HIS3)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530037 pRSBB424 (TRP1)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530038 pRSBB425 (LEU2)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530039 pRSBB426 (URA3)]

Favorite New Parts

Vitamin A Production

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530000 CrtYB] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is a fusion, it is the full Phytoene Synthase enzyme spliced with a Lycopene B-Cyclase enzymatic domain. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530001 CrtE] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
  3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530002 CrtI] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene.

Vitamin C Production

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530025 VTC2] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of GDP-L-Galactose to L-Galactose-1-P.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530026 GME] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose-1-P to L-Galactose.
  3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530027 VTC4] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose to L-Galactono-1,4-Lactone.

Promoters

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530003 KRE9p] Promoter used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p] Promoter used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.

Terminators

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHO1utr] Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1utr] Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.

Vectors

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530032 pRSBB413 HIS3 CEN/ARS Yeast Shuttle Vector] Used for the propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530039 pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector] Used for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Existing Parts Characterized

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_J63006 Gal1p]. This characterization is a work in progress. We will update the part's experience page after the regional jamboree. We are working with the British Columbia team on troubleshooting.



All Submitted Parts
<groupparts>iGEM011 Johns_Hopkins</groupparts>



Retrieved from "http://2011.igem.org/Team:Johns_Hopkins/Data"