Team:Northwestern/Notebook/Week12

From 2011.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 6: Line 6:
<img src="https://static.igem.org/mediawiki/2011/4/40/Notebook_banner4_2.gif" height = "70px" width="750px" style="opacity:1;filter:alpha(opacity=100)" alt="NU-igem banner"/ border="0">
<img src="https://static.igem.org/mediawiki/2011/4/40/Notebook_banner4_2.gif" height = "70px" width="750px" style="opacity:1;filter:alpha(opacity=100)" alt="NU-igem banner"/ border="0">
<div style="margin: -55px 0px 0px 80px;font:35px helvetica; color:#ffffff;">
<div style="margin: -55px 0px 0px 80px;font:35px helvetica; color:#ffffff;">
-
Notebook  <div style="margin: -40px 0px 0px 420px;font:35px helvetica; color:#444444;">    &nbsp; Week 12</div>
+
Notebook  <div style="margin: -40px 0px 0px 420px;font:35px helvetica; color:#444444;">    &nbsp; Week 11</div>
</div></div>
</div></div>
</html>
</html>
Line 12: Line 12:
-
<!--Week 11-->
+
<!--Week 12-->
<!-- ---------------------Day 54 --------------------------------------- -->
<!-- ---------------------Day 54 --------------------------------------- -->
<html><div id="day44" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
<html><div id="day44" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
-
<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 54 - Monday, August 22nd 2011
+
<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 54 - Monday, August 29nd 2011
</div></div></html>
</div></div></html>
-
*More extensive minipreps - this time of all the genomic promoter ligations.  
+
*We decided that our results were looking good, but we needed more replicated of each sample and wanted to test a wider range of autoinducer concentrations. So, we ran a 384 well plate that allowed us to just that.  
-
*Plated our glycerol stocks of the successful gels from friday to make sure everything worked properly.  
+
*We also ran for a longer time (4 hours) to try and see if a sigmoidal flourescence curve developed.
-
*Ran a plate of our controls at the high-throughput lab.  
+
*Began fitting our experimental data into mathematical model
-
*Digested and ran the genomic promoter ligations on a gel
+
<!-- ------------------------------------------------------------------- -->
 +
 
 +
 
 +
<!-- ---------------------Day 55 --------------------------------------- -->
 +
<html><div id="day44" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
 +
<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 55 - Tuesday, August 30th 2011
 +
</div></div></html>
 +
*Continued pouring over all of the data we've acquired so far.
 +
*Began to prepare testing samples for sequencing
 +
*Began overnights so that we could start testing with our RFP samples.
 +
<!-- ------------------------------------------------------------------- -->
 +
 
 +
 
 +
<!-- ---------------------Day 56 --------------------------------------- -->
 +
<html><div id="day44" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
 +
<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 56 - Wednesday, August 31st 2011
 +
</div></div></html>
 +
*Ran another experiment, this time inducing our RFP constructs on a 384 well plate at an OD of around 0.5. Once again, we let the plate run in the High Throughput plate reader for four hours.
 +
*Made more M9 (Testing uses a lot of M9!)
 +
*Decided on questions for Professor Dale Mortenson, a nobel laureate in economics who will be one of the people we interview in our human practices video.
 +
*Planned strategy for getting all of our DNA into a Chlor backbone to submit.
 +
<!-- ------------------------------------------------------------------- -->
 +
 
 +
 
 +
<!-- ---------------------Day 57 --------------------------------------- -->
 +
<html><div id="day44" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
 +
<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 57 - Thursday, September 1st 2011
 +
</div></div></html>
 +
*The RFP testing did not go nearly as well as the GFP. Even some of our controls were incorrect. We are going to run the test again to try and determine the source of error.
 +
*One problem may be that the High-Throughput auto-pipetter did not completely transfer the autoinducer samples.  
 +
*Met with the advisors to discuss the progress of testing
 +
<!-- ------------------------------------------------------------------- -->
 +
 
 +
 
 +
<!-- ---------------------Day 58 --------------------------------------- -->
 +
<html><div id="day44" style="margin: 0px 0px 0px 0px;"><img src="https://static.igem.org/mediawiki/2011/6/6b/Day_banner3.gif" height = "55px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="day banner"/ border="0">
 +
<div style="margin: -40px 0px 18px 20px;font:24px helvetica;font-weight:550; color:#ffffff;">Day 58 - Friday, September 2nd 2011
 +
</div></div></html>
 +
*This is our last full day in lab together as a team! However, we will continue coming in over the next several weeks to finish up our testing, data analysis and human practices.
 +
*Organized the lab and cleaned up a lot
 +
*Ran another 384 well plate with RFP samples
 +
*Submitted our testing samples for sequencing
<!-- ------------------------------------------------------------------- -->
<!-- ------------------------------------------------------------------- -->

Latest revision as of 19:03, 25 September 2011

RETURN TO IGEM 2010



day banner
Day 54 - Monday, August 29nd 2011

  • We decided that our results were looking good, but we needed more replicated of each sample and wanted to test a wider range of autoinducer concentrations. So, we ran a 384 well plate that allowed us to just that.
  • We also ran for a longer time (4 hours) to try and see if a sigmoidal flourescence curve developed.
  • Began fitting our experimental data into mathematical model


day banner
Day 55 - Tuesday, August 30th 2011

  • Continued pouring over all of the data we've acquired so far.
  • Began to prepare testing samples for sequencing
  • Began overnights so that we could start testing with our RFP samples.


day banner
Day 56 - Wednesday, August 31st 2011

  • Ran another experiment, this time inducing our RFP constructs on a 384 well plate at an OD of around 0.5. Once again, we let the plate run in the High Throughput plate reader for four hours.
  • Made more M9 (Testing uses a lot of M9!)
  • Decided on questions for Professor Dale Mortenson, a nobel laureate in economics who will be one of the people we interview in our human practices video.
  • Planned strategy for getting all of our DNA into a Chlor backbone to submit.


day banner
Day 57 - Thursday, September 1st 2011

  • The RFP testing did not go nearly as well as the GFP. Even some of our controls were incorrect. We are going to run the test again to try and determine the source of error.
  • One problem may be that the High-Throughput auto-pipetter did not completely transfer the autoinducer samples.
  • Met with the advisors to discuss the progress of testing


day banner
Day 58 - Friday, September 2nd 2011

  • This is our last full day in lab together as a team! However, we will continue coming in over the next several weeks to finish up our testing, data analysis and human practices.
  • Organized the lab and cleaned up a lot
  • Ran another 384 well plate with RFP samples
  • Submitted our testing samples for sequencing
Retrieved from "http://2011.igem.org/Team:Northwestern/Notebook/Week12"