Team:UC Davis/Data LambdacI

From 2011.igem.org

(Difference between revisions)
 
(17 intermediate revisions not shown)
Line 17: Line 17:
<html>
<html>
-
 
<div class="floatbox">
<div class="floatbox">
-
<h1 id="widget">LacI Promoter Characterization Data</h1>
+
<h1>λ c1 Promoter Data</h1>
-
<div id="mutantwidget" class="floatbox2">
+
<div class="floatbox3">
-
<h3>Wildtype</h3>
+
We are still in the process of screening λ c1 promoters for GFP activity and do not have final data to to present at this time.<br><br>
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
0.99315,
+
-
  0.78019, 
+
-
  0.58405,
+
-
  0.45400, 
+
-
  0.35401,
+
-
  0.29099, 
+
-
  0.13305, 
+
-
</span>
+
-
<span class="numconditions">4</span>
+
-
<!--<span class='xdata_3d'>0, 1, 2, 5</span>
+
-
<span class='ydata_3d'>0.00000,  0.20000,  0.40000,  0.60000,  0.80000,  1.00000,  2.00000</span>
+
-
<span class='zdata_3d'>
+
-
  0.99315,  1.02097,  0.99023,  0.99354,
+
-
  0.78019,  0.97021,  0.96725,  0.96226,
+
-
  0.58405,  0.87100,  0.89795,  0.94529,
+
-
  0.45400,  0.76333,  0.85071,  0.93618,
+
-
  0.35401,  0.65243,  0.75444,  0.88056,
+
-
  0.29099,  0.55454,  0.68266,  0.84441,
+
-
  0.13305,  0.26829,  0.37015,  0.55294</span>--!>
+
-
<h3>Mutant 1</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.68829,
+
-
  0.48655, 
+
-
  0.27198,
+
-
  0.15260, 
+
-
  0.08426,
+
-
  0.05322, 
+
-
  0.01741, 
+
-
</span>
+
-
<h3>Mutant 2</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.68514,
+
-
  0.43727, 
+
-
  0.27724,
+
-
  0.17817, 
+
-
  0.11684,
+
-
  0.08021, 
+
-
  0.03415, 
+
-
</span>
+
-
<h3>Mutant 3</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.67026,
+
-
  0.44322, 
+
-
  0.28504,
+
-
  0.17543, 
+
-
  0.10938,
+
-
  0.07325, 
+
-
  0.02937, 
+
-
</span>
+
-
<h3>Mutant 4</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.31888,
+
-
  0.16625, 
+
-
  0.10228,
+
-
  0.05821, 
+
-
  0.03985,
+
-
  0.02549, 
+
-
  0.01329, 
+
-
</span>
+
-
<h3>Mutant 5</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.63174,
+
-
  0.33524, 
+
-
  0.17440,
+
-
  0.09337, 
+
-
  0.03043,
+
-
  0.03337, 
+
-
  0.01686, 
+
-
</span>
+
-
<h3>Mutant 6</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.42290,
+
-
  0.29347, 
+
-
  0.15733,
+
-
  0.09471, 
+
-
  0.05778,
+
-
  0.03850, 
+
-
  0.01475, 
+
-
</span>
+
-
<h3>Mutant 7</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.58989,
+
-
  0.32402, 
+
-
  0.20047,
+
-
  0.11183, 
+
-
  0.06365,
+
-
  0.03915, 
+
-
  0.01712, 
+
-
</span>
+
-
</div>
+
-
</div>
+
-
<div class="floatbox">
+
Check back here for updates after the American regional comepetition!
-
<center>
+
-
<div class="floatbox3">
+
-
<img src="https://static.igem.org/mediawiki/2011/d/d9/UCD_Mut_graph_lacI.png" width="680" height="350">
+
-
Some preliminary data.  These are LacI promoter mutants.  The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter.  There's still more to come!
+
-
</center>
+
</div>
</div>
</div>
</div>
-
 
-
 
-
 
<style>
<style>

Latest revision as of 01:50, 29 September 2011

Our Sponsors

Start a Family

Got a favorite BioBrick? Check our our process for expanding basic parts into part families.

Criteria

View our judging criteria for iGEM 2011 here.

λ c1 Promoter Data

We are still in the process of screening λ c1 promoters for GFP activity and do not have final data to to present at this time.

Check back here for updates after the American regional comepetition!

Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LambdacI"