27 (June 27, 2011 GDS)

From 2011.igem.org

(Difference between revisions)
 
Line 1: Line 1:
 +
<gallery widths=910px heights=800px>
 +
File:June232427GDS.jpg
 +
</gallery>
 +
'''Safety:'''
'''Safety:'''
''Initial Bleach Kill Curve''
''Initial Bleach Kill Curve''
-
 
-
Sample Bacteria for OD and initial cell number
 
-
:-log phase culture  OD= .194
 
-
:-stationary phase culture OD= .685
 
-
:- No cell count for either (too many)
 
-
 
-
Experiment
 
-
 
-
-Mix 4.5 mL culture with 500&lambda; bleach
 
-
 
-
-Incubate for 15 minutes and plate 1 mL
 
-
 
-
-Collect 1 mL and spin to pellet
 
-
 
-
-Wash twice with TE and resuspend in 100&lambda; LB and then plate
 
-
 
-
:- Result: No Colonies
 
-
 
-
 
-
Note: LB plates had bright pink colonies
 
<gallery widths=910px heights=800px>
<gallery widths=910px heights=800px>
-
File:June232427GDS.jpg
+
File:SJune27GDS.jpg
</gallery>
</gallery>

Latest revision as of 15:50, 24 September 2011

Safety:

Initial Bleach Kill Curve