26 (May 26, 2011 GDS)

From 2011.igem.org

(Difference between revisions)
 
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Created TENS
 
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:4.5 mL TE and
 
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::250uL 10% SDS
 
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::250uL 2N NaOH
 
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:-Spin 4 tubes (1.5mL) 30"
 
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:-Pour off sol'n & resuspend
 
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:-Add
 
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::300uL TENS:    mix
 
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::150uL NaOH:    mix
 
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:-Spin 2.5 min.
 
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:-Transfer supernatant to clean tube and add 1mL of EtOH
 
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:-Mix and pellet DNA by centrifugation for 2-5 min
 
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:-Wash pellet with 70% ethanol and allow pellet to dry
 
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:-Resuspend the pellet in 30λ of the TE
 
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:-Digest 5-10λ
 
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<gallery widths=910px heights=800px>
<gallery widths=910px heights=800px>
File:May26June6GDS.jpg
File:May26June6GDS.jpg
</gallery>
</gallery>

Latest revision as of 15:10, 24 September 2011