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Team:ITESM Mexico/Extras
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| - | + | <html><h1 class="myowntitle">Extras</h1> </html> | |
| + | ----- | ||
| - | |||
| - | + | ::'''Some aditional stuff.''' | |
| - | + | [[File:Giant_microbe.png|center]] | |
| - | + | ==='''Home made quick saliva DNA extraction protocol:'''=== | |
| - | + | ::#Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass | |
| + | ::#Prepare a solution with soap and water in a beaker | ||
| + | ::#Add the soap solution to the last rinse | ||
| + | ::#Add frozen (0°C) etanol to the last rinse | ||
| + | ::#Observe how a DNA thread forms, take it and place it in an eppendorf tube. | ||
| + | |||
| + | |||
| + | ===Abbreviations and glossary:=== | ||
| + | |||
| + | ::*'''Biobrickoatl:'''It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks. | ||
| + | ::*'''Competent cells:''' Cells that can incorporate, in an easier way, foreign DNA into their cell walls. | ||
| + | ::*'''CcaS-EnvZ: '''Is the green light receptor that induces the expresión of phycobilisome linker protein. | ||
| + | ::*'''CFP:''' Cyan Fluorescent Protein | ||
| + | ::*'''Crst:''' Lock for the PbadST (strong, for low concentrations) promoter | ||
| + | ::*'''CrWk:''' Lock for the Pbad WK (weak, for high concentration) promoter | ||
| + | ::*'''GFP:''' Green Fluorescent Protein | ||
| + | ::*'''ho1:''' Heme oxigenase 1 | ||
| + | ::*'''iTaST:''' Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity | ||
| + | ::*'''pBADST:''' Strong arabinose promoter for low concentration detection. | ||
| + | ::*'''pBADWk:''' Weak arabinose promoter for high concentration detection. | ||
| + | ::*'''Pcy4A:''' Phycocyanobilin ferredoxin oxidoreductase. | ||
| + | ::*'''RBS:''' Ribosome binding site | ||
| + | ::*'''TaST:''' Key for the CrST lock | ||
| + | ::*'''TaWk:''' Key for the CrWk lock | ||
| + | ::*'''Term:''' Terminator, not the robot (genetic terminator). | ||
Latest revision as of 03:28, 29 September 2011
ITESM MÉXICO
SensE.coli
Extras
- Some aditional stuff.
Home made quick saliva DNA extraction protocol:
- Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
- Prepare a solution with soap and water in a beaker
- Add the soap solution to the last rinse
- Add frozen (0°C) etanol to the last rinse
- Observe how a DNA thread forms, take it and place it in an eppendorf tube.
Abbreviations and glossary:
- Biobrickoatl:It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
- Competent cells: Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
- CcaS-EnvZ: Is the green light receptor that induces the expresión of phycobilisome linker protein.
- CFP: Cyan Fluorescent Protein
- Crst: Lock for the PbadST (strong, for low concentrations) promoter
- CrWk: Lock for the Pbad WK (weak, for high concentration) promoter
- GFP: Green Fluorescent Protein
- ho1: Heme oxigenase 1
- iTaST: Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
- pBADST: Strong arabinose promoter for low concentration detection.
- pBADWk: Weak arabinose promoter for high concentration detection.
- Pcy4A: Phycocyanobilin ferredoxin oxidoreductase.
- RBS: Ribosome binding site
- TaST: Key for the CrST lock
- TaWk: Key for the CrWk lock
- Term: Terminator, not the robot (genetic terminator).
