Team:Washington/Magnetosomes/Future

From 2011.igem.org

(Difference between revisions)

Latest revision as of 02:16, 29 September 2011

iGEM Toolkits: Future Directions

Even though the end of iGEM 2011 is near, there is still a lot we would like to accomplish. By providing these toolkits, we hope to encourage future iGEM teams to experiment with Gibson cloning to help create large-scale constructs like the genome of Magnetotactic Bacteria.

Gibson Assembly Toolkit

1. Remove XhoI site from pGA3K3 vector:

2. Remove homologous regions from pGA4A5, pGA4C5 vectors: these homologous regions within the backbone are self-complementary and can bind to one another, circularizing the backbone without an insert. This could potentially reduce the backbones efficiency and should be eliminated.

3. Finally, we would like to continue adding more BioBrick compatible Gibson Vectors to help expand their popularity within the iGEM community.

Magnetosome Toolkit

There is still much work to be done before we can achieve our goal of making Magnetocoli; however, we feel our toolkit provides an essential foundation for future magnetosome gene manipulation.

1. Co-express the genes in E.coli to determine their gene interactions.

2. Build the scaffold required for proper magnetosome alignment.

The construction of this scaffold would prove highly applicable to many other systems. For our "Make It" project concerning alkane production, we mentioned our interest in enzyme localization. Using mamI, a membrane localized gene, we hope to devise a system that would allow the localization of AAR and ADC to the fatty-acid rich membrane, thus increasing the efficiency of alkane production.

3. Continue to construct the full mamAB operon of the MAI in thirds.

4. Determine other regions of the MAI that contain additional essential genes for magnetosome formation.

Overall, the ability to produce and control uniform, nano-sized magnetic particles is attractive in areas such as medical imaging and nano-electronics where scientists and engineers are actively seeking innovative solutions for advancements in size and accuracy. Thus, if we are able to produce magnets in an organism that are thoroughly understood in a controlled manner, and be able to extract the magnets from them, this indeed is going to be very useful many projects in synthetic biology.

@@ Line 3: / Line 3: @@
 <center><big><big><big><big>'''iGEM Toolkits: Future Directions'''</big></big></big></big></center><br><br>
+[[File:Washington_iGEM2011_futuretoolkit.png|300px|center]]
+Even though the end of iGEM 2011 is near, there is still a lot we would like to accomplish. By providing these toolkits, we hope to encourage future iGEM teams to experiment with Gibson cloning to help create large-scale constructs like the genome of Magnetotactic Bacteria.
 =='''Gibson Assembly Toolkit'''==
+. '''Remove XhoI site from pGA3K3 vector''':
+. '''Remove homologous regions from pGA4A5, pGA4C5 vectors''': these homologous regions within the backbone are self-complementary and can bind to one another, circularizing the backbone without an insert. This could potentially reduce the backbones efficiency and should be eliminated.
+. Finally, we would like to continue adding more BioBrick compatible Gibson Vectors to help expand their popularity within the iGEM community.
+=='''Magnetosome Toolkit'''==
+There is still much work to be done before we can achieve our goal of making '''Magnetocoli'''; however, we feel our toolkit provides an essential foundation for future magnetosome gene manipulation.
+<br/>
-. Remove XhoI site from pGA3K3 vector
+. Co-express  the genes in E.coli to determine their gene interactions.
-. Remove homologous regions from pGA4A5, pGA4C5 vectors
+. Build the scaffold required for proper magnetosome alignment.
+* The construction of this scaffold would prove highly applicable to many other systems. For our "Make It" project concerning alkane production, we mentioned our interest in [https://2011.igem.org/Team:Washington/Alkanes/Future/Localization enzyme localization]. Using ''mamI'', a membrane localized gene, we hope to devise a system that would allow the localization of AAR and ADC to the fatty-acid rich membrane, thus increasing the efficiency of alkane production.
-We will keep working on adding more components into the toolkits at UW and hope that iGEM community will be able to use toolkits to join in on the fun!
+. Continue to construct the full mamAB operon of the MAI in thirds.
------
+. Determine other regions of the MAI that contain additional essential genes for magnetosome formation.
-<br/>
-;Gibson Assembly Toolkit
-We have developed and submitted several vectors that are Gibson Cloning friendly(see the "parts submitted" page). More of such vectors should be developed and added to the toolkit in the future.
-;Magnetosome Toolkit
-We still have a long way to achieve our goal of making magnetic ''E.coli'' but this project is certainly worth investigating. We have a ton of project ideas for the future teams...
-<br/>
-*Express the rest of the gene in the MAI region in E.coli
-*Co-express the genes and study their interaction
-*Build the scaffold structure in E.coli
-*Express the full assembly in E.coli
-*Develop assay for the magnet formation
-*Determine the optimal cell growth condition......
-In addition, the ability to produce and control uniform, nano-sized magnetic particles is attractive in areas such as medical imaging and nano-electronics where scientists and engineers are actively seeking innovative solutions for breakthrough in size and accuracy. Thus, if we are able to produce magnets in an organism that are thoroughly understood in a controlled manner, and be able to extract the magnets from them, this indeed is going to be very useful for a lot of areas.
+Overall, the ability to produce and control uniform, nano-sized magnetic particles is attractive in areas such as medical imaging and nano-electronics where scientists and engineers are actively seeking innovative solutions for advancements in size and accuracy. Thus, if we are able to produce magnets in an organism that are thoroughly understood in a controlled manner, and be able to extract the magnets from them, this indeed is going to be very useful many projects in synthetic biology.