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Team:Paris Bettencourt/Data
From 2011.igem.org
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<h3>Data for our favorite new parts</h3> | <h3>Data for our favorite new parts</h3> | ||
</html> | </html> | ||
| + | # [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': It's a recombinant fusion protein composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). | ||
# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence. | # [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence. | ||
| + | # [http://partsregistry.org/Part:BBa_K606027 Main Page] - '''pVeg+SpoVG- YFP:tetR- Double Terminator , BBa_K606027''': The YFP:tetR expression system. | ||
<html> | <html> | ||
<h3>Data for Pre-existing Parts</h3> | <h3>Data for Pre-existing Parts</h3> | ||
</html> | </html> | ||
| - | # [http://partsregistry.org/Part: | + | # [http://partsregistry.org/Part:BBa_K143053 Main Page] - '''pVeg + SpoVG, BBa_K143053''': promoter and RBS for B. subtilis (Imperial College, iGEM 2008) |
| + | # [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''': standard double terminator<html> | ||
<html> | <html> | ||
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<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/GFPLac_diffusion">See the design</a></li> | <li><a href="https://2011.igem.org/Team:Paris_Bettencourt/GFPLac_diffusion">See the design</a></li> | ||
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion">See the experiments</a></li> | <li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion">See the experiments</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion_experiments">See the nanotubes experiments</a></li> | ||
</ul> | </ul> | ||
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<h2>The T7 polymerase diffusion system</h2> | <h2>The T7 polymerase diffusion system</h2> | ||
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</html> | </html> | ||
# [http://partsregistry.org/Part:BBa_K606039:Experience Experience] - '''pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039''': T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase. | # [http://partsregistry.org/Part:BBa_K606039:Experience Experience] - '''pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039''': T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase. | ||
| - | # [http://partsregistry.org/Part:BBa_K606036:Experience Experience] - '''pT7 SpoVG T7 SpoVG GFP T7terminator''': T7 and GFP under a pT7 promoter to response a emission of T7. | + | # [http://partsregistry.org/Part:BBa_K606036:Experience Experience] - '''pT7 SpoVG T7 SpoVG GFP T7terminator, BBa_K606036''': T7 and GFP under a pT7 promoter to response a emission of T7. |
<html> | <html> | ||
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<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Modeling/T7_diffusion">See the model</a></li> | <li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Modeling/T7_diffusion">See the model</a></li> | ||
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion">See the experiments</a></li> | <li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion">See the experiments</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion_experiments">See the nanotubes experiments</a></li> | ||
</ul> | </ul> | ||
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# [http://partsregistry.org/Part:BBa_K606034 Main Page] - '''Tyrosine amber supressor tRNA for subtilis, BBa_K606034''': tRNA tyrosine amber compatible with both B. subtilis and E. coli. | # [http://partsregistry.org/Part:BBa_K606034 Main Page] - '''Tyrosine amber supressor tRNA for subtilis, BBa_K606034''': tRNA tyrosine amber compatible with both B. subtilis and E. coli. | ||
# [http://partsregistry.org/Part:BBa_K606032 Main Page] - '''T7 amber, BBa_K606032''': This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber. | # [http://partsregistry.org/Part:BBa_K606032 Main Page] - '''T7 amber, BBa_K606032''': This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber. | ||
| + | # [http://partsregistry.org/Part:BBa_K606064 Main Page] - '''T7 amber with autoamplification loop, BBa_K606064''' | ||
| + | # [http://partsregistry.org/Part:BBa_K606065 Main Page] - '''IPTG inducible tRNA Amber emitter with RFP monitor, BBa_K606065''' | ||
| + | # [http://partsregistry.org/Part:BBa_K606067 Main Page] - '''Upstream LacI expression IPTG inducible tRNA emitter with RFP Monitor, BBa_K606067''' | ||
<html> | <html> | ||
<h3>Data for Pre-existing Parts</h3> | <h3>Data for Pre-existing Parts</h3> | ||
</html> | </html> | ||
| - | # [http://partsregistry.org/Part:BBa_K143053 | + | # [http://partsregistry.org/Part:BBa_K143053 Main Page] - '''pVeg + SpoVG, BBa_K143053''': promoter and RBS for B. subtilis (Imperial College, iGEM 2008) |
| + | # [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''' | ||
<html> | <html> | ||
<br/> | <br/> | ||
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</html> | </html> | ||
# [http://partsregistry.org/Part:BBa_K606044 Main Page] - '''pVeg SpoVg CI TT, BBa_K606044''': Expression casette for for CI in B. subtilis. | # [http://partsregistry.org/Part:BBa_K606044 Main Page] - '''pVeg SpoVg CI TT, BBa_K606044''': Expression casette for for CI in B. subtilis. | ||
| + | # [http://partsregistry.org/Part:BBa_K606068 Main Page] - '''pVeg SpoVg CI SpoVG RFP TT, BBa_K606068''': Expression casette for for CI in B. subtilis with RFP monitor. | ||
<html> | <html> | ||
<h3>Data for Pre-existing Parts</h3> | <h3>Data for Pre-existing Parts</h3> | ||
</html> | </html> | ||
| - | # [http://partsregistry.org/Part:BBa_K143053 | + | # [http://partsregistry.org/Part:BBa_K143053 Main Page] - '''pVeg + SpoVG, BBa_K143053''': promoter and RBS for B. subtilis (Imperial College, iGEM 2008) |
| - | # [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''' | + | # [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''': standard double terminator |
# [http://partsregistry.org/Part:BBa_C0052 Main Page] - '''CI, BBa_B0052''' - CI gene from the lambda phage. | # [http://partsregistry.org/Part:BBa_C0052 Main Page] - '''CI, BBa_B0052''' - CI gene from the lambda phage. | ||
<html> | <html> | ||
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</html> | </html> | ||
| - | # [http://partsregistry.org/Part:BBa_K143079 Experience] - '''Pveg-spoVG GFP expression construct, BBa_K143079''' (Imperial College, iGEM 2008) | + | # [http://partsregistry.org/Part:BBa_K143079 Experience] - '''Pveg-spoVG GFP expression construct, BBa_K143079''' (Imperial College, iGEM 2008) |
<html> | <html> | ||
Latest revision as of 03:07, 29 October 2011
Data page
The YFP concentration system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: It's a recombinant fusion protein composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO).
- [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
- [http://partsregistry.org/Part:BBa_K606027 Main Page] - pVeg+SpoVG- YFP:tetR- Double Terminator , BBa_K606027: The YFP:tetR expression system.
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143053 Main Page] - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
- [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015: standard double terminator
The T7 polymerase diffusion system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606039:Experience Experience] - pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039: T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase.
- [http://partsregistry.org/Part:BBa_K606036:Experience Experience] - pT7 SpoVG T7 SpoVG GFP T7terminator, BBa_K606036: T7 and GFP under a pT7 promoter to response a emission of T7.
The tRNA Amber diffusion system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606040 Main Page] - Promoter Hyperspank, BBa_K606040: IPTG Inducible promoter shortened compared to the one that was before in the registry.
- [http://partsregistry.org/Part:BBa_K606041 Main Page] - pHyperspank Tyrosine amber supressor tRNA double terminator, BBa_K606041: system for using the tRNA in a IPTG inducible system.
- [http://partsregistry.org/Part:BBa_K606034 Main Page] - Tyrosine amber supressor tRNA for subtilis, BBa_K606034: tRNA tyrosine amber compatible with both B. subtilis and E. coli.
- [http://partsregistry.org/Part:BBa_K606032 Main Page] - T7 amber, BBa_K606032: This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber.
- [http://partsregistry.org/Part:BBa_K606064 Main Page] - T7 amber with autoamplification loop, BBa_K606064
- [http://partsregistry.org/Part:BBa_K606065 Main Page] - IPTG inducible tRNA Amber emitter with RFP monitor, BBa_K606065
- [http://partsregistry.org/Part:BBa_K606067 Main Page] - Upstream LacI expression IPTG inducible tRNA emitter with RFP Monitor, BBa_K606067
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143053 Main Page] - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
- [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015
The ComS diffusion system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606037 Main Page] - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
- [http://partsregistry.org/Part:BBa_K606038 Main Page] - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143053 Main Page] - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
- [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015: standard double terminator
- [http://partsregistry.org/Part:BBa_K143021 Main Page] - RFP, BBa_K143021: RFP m-cherry.
- Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [http://www.elowitz.caltech.edu/publications/Tunability.pdf]
- CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [http://www.tufts.edu/sackler//facultyIntros/sonensheinA.html]
The SinOp system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606046 Main Page] - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
- [http://partsregistry.org/Part:BBa_K606047 Main Page] - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter
Data for Pre-existing Parts
- SinOp reporter strain, given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]
The lambda switch system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606044 Main Page] - pVeg SpoVg CI TT, BBa_K606044: Expression casette for for CI in B. subtilis.
- [http://partsregistry.org/Part:BBa_K606068 Main Page] - pVeg SpoVg CI SpoVG RFP TT, BBa_K606068: Expression casette for for CI in B. subtilis with RFP monitor.
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143053 Main Page] - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
- [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015: standard double terminator
- [http://partsregistry.org/Part:BBa_C0052 Main Page] - CI, BBa_B0052 - CI gene from the lambda phage.
GFP diffusion
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143079 Experience] - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008)
