Team:Uppsala-Sweden/Project/Data

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Latest revision as of 13:52, 25 October 2011

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Overview of the system

Data For Our Favorite New Parts

1. Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592009) - amilCP, a blue chromoprotein, BBa_K592009. This chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation.

2. Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592100) - Blue Fluorescent Protein (mTagBFP), BBa_K592100. This part codes for the bright blue fluorescent protein TagBFP. TagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm.

3. Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592200) - Low to medium copy BioBrick standard vector, BBa_K592200. A BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli.

Data For Pre-existing Parts

1. Main page http://partsregistry.org/Part:BBa_R0011 - PLlacO – promoter, BBa_R0011: the promoter show the highest RPU-value of all characterized promoters and had the highest with IPTG.

2. Main Page (http://partsregistry.org/Part:BBa_J23113) - constitutive promoter family member, BBa_J23113 Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. As expected J23113 showed a low promoter strength.

3. Main page http://partsregistry.org/Part:BBa_K592026 J23101-B0032-BFP, BBa_K592026: the constitutive promoter was used as the reference in the characterization measurement.

Characterized Parts

Our measurement with the FACS AriaII shown the following Relative Promoter Units with the Blue Fluorescent Protein (mTagBFP) as reporter, assembled with B0032 in pSB3K3-backbone transformed in TOP10 E.coli-strain.

1. Main page http://partsregistry.org/Part:BBa_K592003 - PcpcG2 promoter, BBa_K592003: measurement shows a RPU-value of 0,1.

2. Main page http://partsregistry.org/Part:BBa_K592006 - PFixK2 promoter, BBa_K592006 is activated by the blue sensor and showed no significant expression in absence of the YF1-sensor (BBa_K592004)

3. Main page http://partsregistry.org/Part:BBa_K592008 - PT5-lac Promoter, BBa_K592008: showed high expression of BFP, and showed higher expression in the presence of IPTG than without it.

@@ Line 17: / Line 17: @@
 <br>
-<h2>Overview of the system</h2>
+<div class="grid_Green_thin omega mywhite">
+Overview of the system
+</div>
 <br>
 [[File:System overview 670.png]]
-<h3>Data For Our Favorite New Parts</h3>
+<div class="grid_Red_thin omega mywhite">
+Data For Our Favorite New Parts
+</div>
 . Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592009) - amilCP, a blue chromoprotein, BBa_K592009.
 This chromoprotein from the coral <i>Acropora millepora</i>, amilCP, naturally exhibits strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation.
-. Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592100) - Blue Fluorescent Protein (mTagBFP), BBa_K592100. This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm.
+. Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592100) - Blue Fluorescent Protein (mTagBFP), BBa_K592100. This part codes for the bright blue fluorescent protein TagBFP. TagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm.
 . Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592200) - Low to medium copy BioBrick standard vector, BBa_K592200. A BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli.
-<h3>Data For Pre-existing Parts</h3>
-. Main Page (http://partsregistry.org/Part:BBa_R0082) - Promoter (OmpR, positive), BBa_R0082.
+<div class="grid_Blue_thin omega mywhite">
-In nature, this promoter is upstream of the ompC porin gene.
+Data For Pre-existing Parts
+</div>
-. Main Page (http://partsregistry.org/Part:BBa_Q04400) - QPI (B0034.C0040.B0015.R0040), BBa_Q04400
+. Main page http://partsregistry.org/Part:BBa_R0011  - PLlacO – promoter,  BBa_R0011: the promoter show the highest RPU-value of all characterized promoters and had the highest with IPTG.
-TetR QPI with strong RBS
 . Main Page (http://partsregistry.org/Part:BBa_J23113) - constitutive promoter family member, BBa_J23113
-Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library.
+Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. As expected J23113 showed a low promoter strength.
-. Main Page (http://partsregistry.org/Part:BBa_I15008) - heme oxygenase (ho1) from Synechocystis, BBa_I15008
+. Main page http://partsregistry.org/Part:BBa_K592026 J23101-B0032-BFP, BBa_K592026: the constitutive promoter was used as the reference in the characterization measurement.
-ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis. A review from the old result system indicates that this part did not work in some test.
-. Main Page (http://partsregistry.org/Part:BBa_I15009) - phycocyanobilin:ferredoxin oxidoreductase, BBa_I15009 Converts biliverdin IXalpha (BV)(produced by Part:BBa_I15008) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes. Associates with Part:BBa_I15010 to transduce a light signal into a genetic response.
-. Main Page (http://partsregistry.org/Part:BBa_K093005) - RFP with RBS, BBa_K093005. RFP, BBa_E1010, with Elowitz RBS, BBa_B0034.
+<div class="grid_Orange_thin omega mywhite">
+Characterized Parts
-. Main page http://partsregistry.org/Part:BBa_R0011  - LacO – promoter,  BBa_R0011: the promoter show the highest RPU-value of all characterized promoters and had the highest with IPTG.
+</div>
-. Main page http://partsregistry.org/Part:BBa_K592026 J23101-B0032-BFP, Bba_K592026: this measurement construct show that constitutive promoter had 1.0 in RPU-value.
-<h3>We´ve Also Characterized the Following Parts</h3>
 Our measurement with the FACS AriaII shown the following  Relative Promoter Units with the Blue Fluorescent Protein (mTagBFP) as reporter, assembled with B0032 in pSB3K3-backbone transformed in TOP10 E.coli-strain.
@@ Line 59: / Line 56: @@
 [[image:Promoter_characterization.PNG|400px]]
+. Main page http://partsregistry.org/Part:BBa_K592003  - PcpcG2 promoter, BBa_K592003: measurement shows a RPU-value of 0,1.
-. Main page http://partsregistry.org/Part:BBa_K592003  - cpcG2 promoter, BBa_K592003: measurement shows a RPU-value of 0,1.
+. Main page http://partsregistry.org/Part:BBa_K592006 - PFixK2 promoter, BBa_K592006 is activated by the blue sensor and showed no significant expression in absence of the YF1-sensor (BBa_K592004)
-. Main page http://partsregistry.org/Part:BBa_K592006 - FixK2 promoter, BBa_K592006 is tightly regulated by the blue sensor and showed no significant expression in absence of the YF1-sensor (BBa_K592004)
-. Main page http://partsregistry.org/Part:BBa_K592008 -  T5-lac Promoter, BBa_K592008: showed higher expression in the presence of  IPTG than without it.
+. Main page http://partsregistry.org/Part:BBa_K592008 -  PT5-lac Promoter, BBa_K592008: showed high expression of BFP, and showed higher expression in the presence of  IPTG than without it.
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