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Team:Paris Bettencourt/Experiments/Methodologies/K090403J
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| + | <h1>Integration plasmid</h1> | ||
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| + | In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team. | ||
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| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |+ Bacillus subtilis integration vector | ||
| + | |- | ||
| + | |[[File:PBSINT1_final.png|900px|thumb|center|]] | ||
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| + | |- | ||
| + | |} | ||
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| + | <p>At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (<partinfo>K143001</partinfo>).</p> | ||
| + | <p>After, we want to clone them into the integrative plasmid (<partinfo>K090403</partinfo>).</p> | ||
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Latest revision as of 02:34, 22 September 2011
Integration plasmid
In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
 |
At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (<partinfo>K143001</partinfo>).
After, we want to clone them into the integrative plasmid (<partinfo>K090403</partinfo>).
