Team:SouthBend-Mishawaka-HS/Project

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Latest revision as of 17:07, 24 June 2011

Home	The Team	The Project	Notebook	Safety	Model	Parts	Sponsors

The bacteria Pseudomonas aeruginosa is an ubiquitous environmental pathogen that is one of the top three causes of opportunistic human infection. Often it is found in water, soil, skin flora, and most man-made environments. It is a frequent cause of infections such as pneumonia, swimmer’s ear, urinary tract infections (UTIs), and bacteremia. For this reason, chemiluminescence and Lux system parts have been pulled and built into mutated Escherichia coli that can detect the presence of P. aeruginosa.

P. aeruginosa is a Gram-negative aerobic rod. Like others in its genus, it is a free-living bacteria. It is known, however, as a true plant pathogen and increasingly as a disease-causer in animals. This opportunistic pathogen is gaining increasing attention. It rarely infects healthy tissue, but there is little tissue it cannot infect if the host tissue is compromised. It is becoming increasingly common in severe burn patients, and patients with cancer or AIDS who are immunosuppressed. Case fatality rate in these pacients is nearing 50%.

Source:http://www.textbookofbacteriology.net/pseudomonas.html

Because of the increasing severity of this pathogen, we are creating a device to detect it. We are using E. coli as the base of our machine. It detects P. aeruginosa by quorum sensing factors and reports using the gene for Green Flurorescent Protein. Ultimately, we would like to create a device that detects and measures the glow to determine, not only if P. aeruginosa is present, but also an approximation of the concentration. We would like to have a small, handheld device like the glucometer shown in the picture to the right.

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-[[Image:Topper Project.jpg|center]]
+[[Image:Topper Home.jpg|center]]
-{| style="color:light blue;background-color:#048E57;" cellpadding="3" cellspacing="1" border="1" bordercolor="#0066FF" width="90%" align="center"
+{| style="color:light blue;background-color:#048E57;" cellpadding="3" cellspacing="1" border="1" bordercolor="#0066FF" width="100%" align="center"
 !align="center"|[[Team:SouthBend-Mishawaka-HS|<font color=#99FFFF >Home</font>]]
 !align="center"|[[Team:SouthBend-Mishawaka-HS/Team|<font color=#99FFFF >The Team</font>]]
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 !align="center"|[[Team:SouthBend-Mishawaka-HS/Model|<font color=#99FFFF>Model</font>]]
 !align="center"|[[Team:SouthBend-Mishawaka-HS/Parts|<font color=#99FFFF >Parts</font>]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS/Attributions|<font color=#99FFFF >Sponsors</font>]]
 |}
-{|align="justify"
+<br><br>
-|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+[[File:Pseudumonas aeruginosa 2.jpg|250px|right]]
-|[[Image:SouthBend-Mishawaka-HS_logo.png|200px|right|frame]]
-|-
-|
-''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
-|[[Image:IMG 0054.JPG|right|frame|Your team picture]]
-|-
-|
-|align="center"|[[Team:SouthBend-Mishawaka-HS | Team Example]]
-|}
-== '''Overall project''' ==
-The bacteria ''Pseudomonas aeruginosa'' is an ubiquitous environmental pathogen that is one of the top three causes of opportunistic human infection. Often it is found in water, soil, skin flora, and most man-made environments. It is a frequent cause of infections such as pneumonia, swimmer’s ear, urinary tract infections (UTIs), and bacteremia. For this reason, chemiluminescence and Lux system parts have been pulled and built into mutated ''Escherichia coli'' that can detect the presence of ''P. aeruginosa''.
-== Project Details==
-=== Part 2 ===
-=== The Experiments ===
-=== Part 3 ===
+<big>The bacteria ''Pseudomonas aeruginosa'' is an ubiquitous environmental pathogen that is one of the <u><b>top three</b></u> causes of opportunistic human infection. Often it is found in water, soil, skin flora, and most man-made environments. It is a frequent cause of infections such as <u>pneumonia, swimmer’s ear, urinary tract infections (UTIs), and bacteremia</u>. For this reason, chemiluminescence and Lux system parts have been pulled and built into mutated ''Escherichia coli'' that can detect the presence of ''P. aeruginosa''.</big><br><br><br><br>
+[[File:Pseudumonas_aeruginosa.jpg|150px|left]]
+<big><i>P. aeruginosa</i> is a Gram-negative aerobic rod. Like others in its genus, it is a free-living bacteria. It is known, however, as a true plant pathogen and increasingly as a disease-causer in animals. This opportunistic pathogen is gaining increasing attention. It rarely infects healthy tissue, but there is little tissue it cannot infect if the host tissue is compromised. It is becoming increasingly common in severe burn patients, and patients with cancer or AIDS who are immunosuppressed. Case fatality rate in these pacients is nearing 50%.</big><br><br><br><br><br><small>Source:http://www.textbookofbacteriology.net/pseudomonas.html</small><br><br>
+[[File:glucometer.jpg|200px|right]]
-== Results ==
+<big>Because of the increasing severity of this pathogen, we are creating a device to detect it. We are using <i>E. coli</i> as the base of our machine. It detects <i>P. aeruginosa</i> by quorum sensing factors and reports using the gene for Green Flurorescent Protein. Ultimately, we would like to create a device that detects and measures the glow to determine, not only if <i>P. aeruginosa</i> is present, but also an approximation of the concentration. We would like to have a small, handheld device like the glucometer shown in the picture to the right.</big>