Team:Freiburg/Notebook/22 August

From 2011.igem.org

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{{:Team:Freiburg/Templates/header}}
{{:Team:Freiburg/Templates/header}}
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<div id="notebook-page-header">
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<div id="notebook-back" width="100px" >
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/21_August">Previous entry</a>
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<div id="notebook-title">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 22 August </a>
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<div id="notebook-next">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/23_August">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
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===Transformation===
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{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name:Rüdiger
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date:22.08
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|-
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| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Continue from Date 19.08. Name Rüdiger
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Experiment Ligation
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|-
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| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name: Precipitator
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|}
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Procedure
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# take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells)
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# thaw cells on ice 20 minutes
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# pipette 50 μl cells and 2 μl DNA into eppi still on ice!
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# Incubate for 30 minutes on ice
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# Heat at 42°C for 60 sec
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# Incubate on ice for 5 minutes
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# Add 200 μl LB Broth
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# Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!)
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# Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance
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'''Documentation:'''
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Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc.
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{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| pSB1C3
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61a,b
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62a.,b
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64a,b
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repetition from 20.08.
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Miniprep: Julia 24.05.
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|}
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===Transformation===
===Transformation===

Latest revision as of 01:11, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!