Team:Bilkent UNAM Turkey
From 2011.igem.org
(Difference between revisions)
(45 intermediate revisions not shown) | |||
Line 181: | Line 181: | ||
<div class='sliding_menu'> | <div class='sliding_menu'> | ||
<span class='sliding_item'> | <span class='sliding_item'> | ||
- | <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Project_Overview"> | + | <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Project_Overview">Background</a> |
+ | </span> | ||
+ | <span class='sliding_item'> | ||
+ | <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Project_Description">Project_Description</a> | ||
</span> | </span> | ||
</div> | </div> | ||
Line 205: | Line 208: | ||
</span> | </span> | ||
<span class='sliding_item'> | <span class='sliding_item'> | ||
- | <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/ | + | <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Safety">Lab Safety</a> |
</span> | </span> | ||
</div> | </div> | ||
</span> | </span> | ||
<span class='tab_item'> | <span class='tab_item'> | ||
- | + | HUMAN PRACTICE | |
<div class='sliding_menu'> | <div class='sliding_menu'> | ||
<span class='sliding_item'> | <span class='sliding_item'> | ||
Line 220: | Line 223: | ||
<span class='sliding_item'> | <span class='sliding_item'> | ||
<a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/iGEM_Everywhere">iGEM Everywhere</a> | <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/iGEM_Everywhere">iGEM Everywhere</a> | ||
+ | </span> | ||
+ | <span class='sliding_item'> | ||
+ | <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Collaboration">Collaboration</a> | ||
</span> | </span> | ||
</div> | </div> | ||
Line 246: | Line 252: | ||
- | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>Biodegradation of TNT and TNT derivatives by <i>nfsI</i>-transfected <i> | + | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>Biodegradation of TNT and TNT derivatives by <i>nfsI</i>-transfected <i>Chlamydomonas_reinhardtii</i><o:p></o:p></span></b></p> |
<p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>Abstract<o:p></ | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>Abstract<o:p></ | ||
o:p></span></b></p> | o:p></span></b></p> | ||
+ | |||
<table width="880" height="225" cellpadding="5" cellspacing="0" style="background:transparent !important;"> | <table width="880" height="225" cellpadding="5" cellspacing="0" style="background:transparent !important;"> | ||
- | + | <tr> | |
- | <td> | + | <td rowspan="2"> <img src="https://static.igem.org/mediawiki/2011/5/54/But.png" width="370" height="340"></td> |
- | + | ||
+ | <td><img src="https://static.igem.org/mediawiki/2011/e/eb/Nbt1201-1168-F1.gif" width="400"></td> | ||
+ | </tr> | ||
- | + | <tr><td> | |
- | < | + | Our aim is to modify the unicellular microalga <i>Chlamydomonas reinhardtii</i> for effective trinitrotoluene (TNT) biodegradation by expression of Enterobacter cloacae nitroreductase gene nfsI. <i>C. reinhardtii</i> is a ubiquitous species capable of thriving in soil, freshwater and marine environments, making this alga a suitable choice for removal of TNT from a wide variety of biomes. We have opted for the use of nfsI as the gene in question is a well-characterized nitroreductase with a known sequence. Our experimental procedure involves (a) development of a synthetic nfsI gene with flanking prefix and suffixes of standard Biobricks, (b) ligation of this sequence to the C. heinhardtii expression vector pRbcBRL, (c) transfection of <i>C. reinhardtii</i> with the recombinant plasmid and (d) TNT tolerance and biodegradation capacity assessment of the modified alga. |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</td> | </td> | ||
- | + | </tr> | |
+ | |||
</table> | </table> | ||
Line 282: | Line 281: | ||
</li> | </li> | ||
- | <li> | + | <li>Bilkent UNAM Turkey team established in January 2011 and member list has grown and changed in time. We shared a lot by that time and are extremely thankful to our former members. We did many brainstorming meetings, some of them was inconclusive. Team went through several problems that cause slightly negative impact on team. For a team to join from Turkey, money always becomes a problem. That’s why we are just able to send just two of our members to the regional jamboree. Moreover, laboratory equipment shipping takes very much time, even a restriction enzyme received one month after the ordering. Our team survive those restrictions and did its best. The force that always give impetus to ourselves is the friendship that we have. |
</li> | </li> | ||
</td> | </td> | ||
Line 310: | Line 309: | ||
<ul> | <ul> | ||
<li> | <li> | ||
- | <p> IGEM Project is our number one priority. As we worked really hard for our project it is important for us to make our dreams come true which is taking a gold medal. Questioning different items like experiment time, materials and methods and the efficiency of the experiments improved our skills and we started to look as a scientist to problems we faced. There were lots of challenging parts and sometimes we thought to quit but we listened to “Eye of The Tiger” and we did not give up from our experiments we delayed them yes, but we never gave up. <p> | + | <p> |
+ | |||
+ | IGEM Project is our number one priority. As we worked really hard for our project it is important for us to make our dreams come true which is taking a gold medal. Questioning different items like experiment time, materials and methods and the efficiency of the experiments improved our skills and we started to look as a scientist to problems we faced. There were lots of challenging parts and sometimes we thought to quit but we listened to “Eye of The Tiger” and we did not give up from our experiments we delayed them yes, but we never gave up. <p> | ||
IGEM team- meet-up was a great opportunity for us to communicate with the other groups, understood their projects and share our ideas. As we had a chance for an interview with Anatolia Agency, we also used media power to get attention to our project, iGEM and the importance of synthetic biology. <p> | IGEM team- meet-up was a great opportunity for us to communicate with the other groups, understood their projects and share our ideas. As we had a chance for an interview with Anatolia Agency, we also used media power to get attention to our project, iGEM and the importance of synthetic biology. <p> | ||
Apart from that we did not forget having fun apart from our lab. As we planned a BBQ party, it was also a great chance for us to relax and gain some energy for our experiments, I did not even mention about the delicious foods and how joyful was our activities. Also like in iGEM team meeting up, we had a chance to introduce our project and main aim to other scientists and researchers in different areas.<p> | Apart from that we did not forget having fun apart from our lab. As we planned a BBQ party, it was also a great chance for us to relax and gain some energy for our experiments, I did not even mention about the delicious foods and how joyful was our activities. Also like in iGEM team meeting up, we had a chance to introduce our project and main aim to other scientists and researchers in different areas.<p> | ||
Links at above lead you to specific event and its photos suppose to be at that link. If not, look at gallery link. | Links at above lead you to specific event and its photos suppose to be at that link. If not, look at gallery link. | ||
Thanks and get great time.<p> | Thanks and get great time.<p> | ||
- | |||
</li> | </li> | ||
</ul> | </ul> | ||
Line 323: | Line 323: | ||
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Bilkent_UNAM_Turkey"><img src="https://static.igem.org/mediawiki/2011/e/ea/Our_parts.png" width="700"> </img></a> | <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Bilkent_UNAM_Turkey"><img src="https://static.igem.org/mediawiki/2011/e/ea/Our_parts.png" width="700"> </img></a> | ||
- | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>Favorite Parts<p> | + | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>Favorite Parts<p></b> |
- | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>BBa_K596001<p> | + | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>BBa_K596001<p></b> |
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K596001"><img src="https://static.igem.org/mediawiki/2011/f/f3/BBa_K596001.JPG" width="700" height="300"> </img></a> | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K596001"><img src="https://static.igem.org/mediawiki/2011/f/f3/BBa_K596001.JPG" width="700" height="300"> </img></a> | ||
- | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>BBa_K596004<p> | + | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>BBa_K596004<p></b> |
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K596004"><img src="https://static.igem.org/mediawiki/2011/0/05/Algae_protein_expression_vector_pAPEV.png" width="450" height="450"> </img></a> | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K596004"><img src="https://static.igem.org/mediawiki/2011/0/05/Algae_protein_expression_vector_pAPEV.png" width="450" height="450"> </img></a> | ||
- | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>DNA Submission to Registry<p> | + | <p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>DNA Submission to Registry<p></b> |
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2011/9/9f/DNA_submission_to_registry.JPG" width="700"><p> | ||
+ | |||
- | |||
Line 354: | Line 356: | ||
<ul> | <ul> | ||
<li> | <li> | ||
- | We | + | We find it our duty to thank Gulce Itir Percin, Aydan Torun and Ömer Faruk |
- | + | Sarıoglu for their valuable assistance in conducting the experiments | |
- | + | within our project’s scope, without which we could scarcely have expected | |
+ | to complete our project in time. We must also extend our thanks to | ||
+ | Sustainable Technologies Laboratory Manager Zeynep E. Ülger for providing | ||
+ | the necessary instructions and training for the use of a wide variety of | ||
+ | laboratory equipment. We would like to acknowledge Prof. Dr. Tayfun Özçelik and Prof. Dr. Engin Umut Akkaya for their sincere support on our team and efforts for getting finanical support from Bilkent University. We thank the Turkish National Nanotechnology | ||
+ | Institute (UNAM) for the administration of routine training sessions regarding | ||
+ | laboratory safety, which allowed all our team members to operate without | ||
+ | any personal danger throughout the experiments so far. We are also | ||
+ | grateful to Assistant Professor Ayse Begum Tekinay and her laboratory for | ||
+ | supplying much-needed reagents and assistance, both of which allowed our | ||
+ | small team to work much faster than it otherwise could. And lastly we must | ||
+ | thank our supervisor Turgay Tekinay for making this project possible and | ||
+ | granting us a summer that was as entertaining as it was informative. | ||
<h1>Sponsors</h1> | <h1>Sponsors</h1> | ||
- | <img src=" | + | <a href="http://www.bilkent.edu.tr"><img src="http://www.bilkent.edu.tr/~yayinbir/amblem/ing-amblem.jpg" height="100"></img></a> |
- | < | + | <a href="http://www.nano.org.tr/index1.html"><img src="http://www.nano.org.tr/files/Unam_logo.jpg" height="100"></img></a> |
- | <img src="https://static.igem.org/mediawiki/2011/8/8d/Sentegen_logo.jpg" width="150" height="100"></img> | + | <a href="http://www.sentegen.com/"><img src="https://static.igem.org/mediawiki/2011/8/8d/Sentegen_logo.jpg" width="150" height="100"></img></a> |
+ | <h2>Share</h2> | ||
+ | <span class='st_email_large' ></span><span class='st_facebook_large' ></span><span class='st_twitter_large' ></span></span><span class='st_yahoo_large' ></span><span class='st_friendfeed_large' ></span><span class='st_blogger_large' ></span><span class='st_delicious_large' ></span><span class='st_linkedin_large' ></span> | ||
+ | <g:plusone></g:plusone> | ||
</li> | </li> | ||
</ul> | </ul> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</div> | </div> | ||
Line 426: | Line 437: | ||
var td5 = document.createElement('td'); | var td5 = document.createElement('td'); | ||
td5.setAttribute('width','110'); | td5.setAttribute('width','110'); | ||
- | |||
- | |||
href1.appendChild(image1); | href1.appendChild(image1); | ||
Line 487: | Line 496: | ||
</script> | </script> | ||
+ | <script type="text/javascript">var switchTo5x=true;</script><script type="text/javascript" src="http://w.sharethis.com/button/buttons.js"></script><script type="text/javascript">stLight.options({publisher:'fc7a483f-b897-4a2e-9b57-bb2baf6e43da'});</script> | ||
</html> | </html> |
Latest revision as of 03:44, 22 September 2011
There is a problem with poping up if you see this note.