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Team:Imperial College London/Templates/Kill
From 2011.igem.org
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<img class="border" src="https://static.igem.org/mediawiki/2011/5/5d/New_storyboard_v2-09.png" width="170px" style="float:right;margin:23px 20px 0px 20px;" /> | <img class="border" src="https://static.igem.org/mediawiki/2011/5/5d/New_storyboard_v2-09.png" width="170px" style="float:right;margin:23px 20px 0px 20px;" /> | ||
<h1>Module 3: Gene Guard</h1> | <h1>Module 3: Gene Guard</h1> | ||
| - | <p><b>Containment is a serious issue concerning the release of GMOs into the environment. To prevent horizontal gene transfer of the genes we | + | <p><b>Containment is a serious issue concerning the release of genetically modified organisms (GMOs) into the environment. To prevent horizontal gene transfer of the genes we are expressing in our chassis, we have developed a system based on the genes encoding holin, anti-holin and endolysin. We are engineering anti-holin into the genome of our chassis, where it acts as an anti-toxin, and holin and endolysin on plasmid DNA. In the event of horizontal gene transfer with a soil bacterium, holin and endolysin will be transferred without anti-holin, rendering the recipient cell non-viable and effectively containing the Auxin Xpress and Phyto-Route genes in our chassis.</b></p> |
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Latest revision as of 22:42, 21 September 2011
Module 3: Gene Guard
Containment is a serious issue concerning the release of genetically modified organisms (GMOs) into the environment. To prevent horizontal gene transfer of the genes we are expressing in our chassis, we have developed a system based on the genes encoding holin, anti-holin and endolysin. We are engineering anti-holin into the genome of our chassis, where it acts as an anti-toxin, and holin and endolysin on plasmid DNA. In the event of horizontal gene transfer with a soil bacterium, holin and endolysin will be transferred without anti-holin, rendering the recipient cell non-viable and effectively containing the Auxin Xpress and Phyto-Route genes in our chassis.
