"
Team:Paris Liliane Bettencourt/Notebook/2011/07/14/
From 2011.igem.org
(Difference between revisions)
LauradaSilva (Talk | contribs) |
LauradaSilva (Talk | contribs) |
||
| Line 1: | Line 1: | ||
{{:Team:Paris_Bettencourt/tpl_test}} | {{:Team:Paris_Bettencourt/tpl_test}} | ||
| - | ==Kevin== | + | ==Kevin== |
| - | '''Miniprep of lacO/tetO plasmids :''' | + | '''Miniprep of lacO/tetO plasmids :''' |
*Centrifugation of glycerol culturs 4000 RPM - 10 min | *Centrifugation of glycerol culturs 4000 RPM - 10 min | ||
*Put liquid on trash and vortex it | *Put liquid on trash and vortex it | ||
Latest revision as of 15:58, 21 September 2011
Contents |
Kevin
Miniprep of lacO/tetO plasmids :
- Centrifugation of glycerol culturs 4000 RPM - 10 min
- Put liquid on trash and vortex it
- Protocol of QIAprep Spin Miniprep Kit using a microcentrifuge
Mathias & Laura
Reporter BioBricks
Miniprep
-RFP (m-cherry) E1010
-Standard Terminator B0015
-GFP mut3b E0040
-T7 Terminator J18913
-pSB1C3
Digestion and gel extraction
-RFP (m-cherry) by EcoR1 and Spe1
-Standard Terminator by EcoR1 and Xba1
-GFP mut3b by EcoR1 and Spe1
-T7 Terminator by EcoR1 and Xba1
-pSB1C3 by EcoR1 and Pst1
Ligation and transformation
-RFP & Standard Terminator => K606012
-GFP mut3b & T7 Terminator => K606014
-pSB1C3 & RBS => K606061
