Team:Osaka/week6

From 2011.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 1: Line 1:
{{Osaka}}
{{Osaka}}
-
 
+
__NOTOC__
-
 
+
==September 4 (Sun)==
-
==September 4(Sun)==
+
# Restriction digests:3-10C,1-2M
# Restriction digests:3-10C,1-2M
# Gel extraction of digests
# Gel extraction of digests
Line 10: Line 9:
# Photospectrometry measuament
# Photospectrometry measuament
-
==September 5(Mon)==
+
==September 5 (Mon)==
# Absorbance measurement
# Absorbance measurement
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
-
|+Table 7
 
-
!Name!!IPTG(0.1M)!!incubation!!OD600!!A474
 
-
|-
 
-
|1|| -||-||0.8583||0.1567
 
-
|-
 
-
|2||400μl||2h||1.1523||0.1447
 
-
|-
 
-
|3||400μl||1h||0.5583||0.1126
 
-
|-
 
-
|4||40μl||2h||1.0297||0.1878
 
-
|-
 
-
|5||40μl||1h||0.6576||0.1736
 
-
|}
 
# To liquid culture:03,17,3-10C
# To liquid culture:03,17,3-10C
-
==September 6(Tue)==
+
==September 6 (Tue)==
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
Line 44: Line 29:
-
==September 7(Wed)==
+
==September 7 (Wed)==
# PCR
# PCR
# Gel electrophoresis of PCR products
# Gel electrophoresis of PCR products
Line 63: Line 48:
# PCR
# PCR
-
==September 8(Thu)==
+
==September 8 (Thu)==
# Electrophoresis of PCR products
# Electrophoresis of PCR products
# Restriction digests of 1-3A(backbone)
# Restriction digests of 1-3A(backbone)
Line 78: Line 63:
-
==September 9(Fri)==
+
==September 9 (Fri)==
# PCR
# PCR
# Electrophoresis of PCR products  
# Electrophoresis of PCR products  
Line 92: Line 77:
# Transformation of ligation products
# Transformation of ligation products
# Absorbance measurement
# Absorbance measurement
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
-
|+Table 8
 
-
!Name!!UV irradiation!!incubation!!OD600!!A474
 
-
|-
 
-
|03①|| -||-||  ||
 
-
|-
 
-
|03②||1mJ/cm2||1h||  ||
 
-
|-
 
-
|04①|| -||-||  ||
 
-
|-
 
-
|04②||1mJ/cm2||1h||  ||
 
-
|}
 
-
==September 10(Sat)==
+
==September 10 (Sat)==
# PCR
# PCR
# Electrophoresis of PCR products  
# Electrophoresis of PCR products  
Line 112: Line 85:
# PCR purification
# PCR purification
# Absorbance measurement
# Absorbance measurement
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
-
|+Table 9
 
-
!Name!!UV irradiation!!incubation!!OD600!!A474
 
-
|-
 
-
|03①|| -||-||  ||
 
-
|-
 
-
|03②||1mJ/cm2||1h||  ||
 
-
|-
 
-
|04①|| -||-||  ||
 
-
|-
 
-
|04②||1mJ/cm2||1h||  ||
 
-
|}
 
Line 129: Line 90:
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]
-
week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|'''6'''|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]|[[Team:Osaka/week14|14]]
+
week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|'''6'''|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]

Latest revision as of 05:51, 19 October 2011

September 4 (Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5 (Mon)

  1. Absorbance measurement
  2. To liquid culture:03,17,3-10C


September 6 (Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • all parts ×
  4. PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
  5. Gel Electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  6. PCR
  7. Gel Electrophoresis of PCR products
    • ×


September 7 (Wed)

  1. PCR
  2. Gel electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  3. Gel electrophoresis of PprM
  4. Gel extraction
  5. PCR
  6. Gel electrophoresis of PCR products
    • PprI,PprA,RecA ×
  7. Miniprep of yesterday's culture
  8. Restriction digests of miniprepped parts
  9. Gel electrophoresis of digests
    • 03 ok
  10. To liquid culture:03,04 (preincubation)
  11. PCR
  12. Electrophoresis of PCR products
  13. PCR

September 8 (Thu)

  1. Electrophoresis of PCR products
  2. Restriction digests of 1-3A(backbone)
  3. PCR
  4. Electrophoresis of PCR products and miniprepped parts
  5. Gel extraction of 1-3A
  6. PCR
  7. Electrophoresis of PCR products
  8. Inoculation of 03,04
  9. To liquid culture 03,04
  10. PCR
  11. Electrophoresis of PCR products


September 9 (Fri)

  1. PCR
  2. Electrophoresis of PCR products
  3. Gel extraction
  4. Restriction digests
  5. PCR
  6. Electrophoresis of PCR products
  7. Gel extraction
  8. Restriction digests
  9. Ligation
    • 101(C):PprI+1-3A(vector)
    • 102(C):PprA+1-3A(vector)
  10. Transformation of ligation products
  11. Absorbance measurement

September 10 (Sat)

  1. PCR
  2. Electrophoresis of PCR products
  3. To liquid culture:101,102
  4. PCR
  5. PCR purification
  6. Absorbance measurement


Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13

Retrieved from "http://2011.igem.org/Team:Osaka/week6"