Team:UNITS Trieste/Acknowledgements
From 2011.igem.org
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+ | <div id="project-description" role="description"> | ||
+ | <div class="site-title" id="AKs"> | ||
+ | <span>SINBIOME ACKNOWLEDGEMENTS</span> | ||
+ | </div> | ||
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<div id="judging" class="slide4 full-slides"> | <div id="judging" class="slide4 full-slides"> | ||
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<span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553001" target="_blank">BBa_K553001</a></span><br/> | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553001" target="_blank">BBa_K553001</a></span><br/> | ||
<span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553002" target="_blank">BBa_K553002</a></span><br/> | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553002" target="_blank">BBa_K553002</a></span><br/> | ||
+ | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553003" target="_blank">BBa_K553003</a></span><br/> | ||
+ | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553004" target="_blank">BBa_K553004</a></span><br/> | ||
<span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553005" target="_blank">BBa_K553005</a></span><br/> | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553005" target="_blank">BBa_K553005</a></span><br/> | ||
<span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553007" target="_blank">BBa_K553007</a></span><br/> | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553007" target="_blank">BBa_K553007</a></span><br/> | ||
<span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553008" target="_blank">BBa_K553008</a></span><br/> | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553008" target="_blank">BBa_K553008</a></span><br/> | ||
<span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553009" target="_blank">BBa_K553009</a></span><br/> | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553009" target="_blank">BBa_K553009</a></span><br/> | ||
+ | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553010" target="_blank">BBa_K553010</a></span><br/> | ||
<span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553020" target="_blank">BBa_K553020</a></span><br/> | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553020" target="_blank">BBa_K553020</a></span><br/> | ||
<span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553021" target="_blank">BBa_K553021</a></span><br/> | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553021" target="_blank">BBa_K553021</a></span><br/> | ||
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+ | <span class="listlist"><a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553023" target="_blank">BBa_K553023</a></span><br/> | ||
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<a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553008" target="_blank">BBa_K553008</a><br/> | <a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553008" target="_blank">BBa_K553008</a><br/> | ||
+ | <a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553020" target="_blank">BBa_K553020</a><br/> | ||
+ | <a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553021" target="_blank">BBa_K553021</a><br/> | ||
+ | <a class="link_ak" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K553023" target="_blank">BBa_K553023</a><br/> | ||
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IGEM more than a competition is a life experience; it involves you at all, sometimes it is hard sometimes funny but above all it is by far the best experience which a student could done during his undergraduate studies. The UNITS_TRIESTE Team is an “Handmade” team, no advisors working in synthetic biology field, no company linked to synthetic biology business only 6 students and their mighty wish to take their place in a worldwide acknowledged competition as IGEM is.<br/> | IGEM more than a competition is a life experience; it involves you at all, sometimes it is hard sometimes funny but above all it is by far the best experience which a student could done during his undergraduate studies. The UNITS_TRIESTE Team is an “Handmade” team, no advisors working in synthetic biology field, no company linked to synthetic biology business only 6 students and their mighty wish to take their place in a worldwide acknowledged competition as IGEM is.<br/> | ||
<br/> | <br/> | ||
- | <span style=" display: block;text-align: center;"><b>Synbiome is made of | + | <span style=" display: block;text-align: center;"><b>Synbiome is made of "undergraduate" thoughts and it has been realized by "undergraduate" hands.</b></span> |
<br/> | <br/> | ||
As any researchers know three months are nothing, only 90 days tu build up a new, innovative and competitive project to face the world of synthetic biology.<br/> | As any researchers know three months are nothing, only 90 days tu build up a new, innovative and competitive project to face the world of synthetic biology.<br/> | ||
We decided to do it as it must be do, no shortcuts: 6 month of preliminary work making reality the wish to partecipate at igem, learning about everything as biobricks, sponsors, registry, registration, human practice, tracks, jamboree, team and whatever else but mostly we thought about the project, tons of rough ideas falling down as bricks (Not yet so “Bio”J), looking for the best way to handle our first partecipation. At the end of june Synbiome was birth, son of all our minds and father of all our expectation.<br/> | We decided to do it as it must be do, no shortcuts: 6 month of preliminary work making reality the wish to partecipate at igem, learning about everything as biobricks, sponsors, registry, registration, human practice, tracks, jamboree, team and whatever else but mostly we thought about the project, tons of rough ideas falling down as bricks (Not yet so “Bio”J), looking for the best way to handle our first partecipation. At the end of june Synbiome was birth, son of all our minds and father of all our expectation.<br/> | ||
- | Thanks to Synbiome we had the chance to involve scientists originally unrelated to the field of synthetic biology as our instructors. They believed so much our project that we’re not only working at Synbiome in a competitive optical but mostly looking forward to reach a publication at the end of the year.<br/> | + | <b>Thanks to Synbiome we had the chance to involve scientists originally unrelated to the field of synthetic biology as our instructors. They believed so much our project that we’re not only working at Synbiome in a competitive optical but mostly looking forward to reach a publication at the end of the year.</b><br/> |
These rows have been written only to give you all the feelings that this experience has meant for us, we really hope that you will enjoy Synbiome.<br/> | These rows have been written only to give you all the feelings that this experience has meant for us, we really hope that you will enjoy Synbiome.<br/> | ||
+ | </p></blockquote> | ||
+ | |||
+ | <b>Looking for Boston</b> | ||
+ | <blockquote><p> | ||
+ | During the Regional Jamboree in Amsterdam we focused our attention in collecting any kind of feedbacks from everybody: judges, instructors, advisors and other igemmers.<br/> | ||
+ | This has been really useful also to plan our work during this supplementary month.<br/> | ||
+ | Looking for Boston we decided to change the genetic circuit inside the bacterial strain A.<br/> | ||
+ | At the beginning of this experience we believed that forcing a strong mutualism between the two bacterial strains would represent the best way to build a stable consortium. Following this purpose we designed for Amsterdam a bacterial strain A, which in response to OXOC8 produces OXOC12 and glucosidase and a bacterial strain B, which produces OXOC8 and glucosidase in presence of OXOC12. Both A and B are not sensible to their own language and so A needs B and vice versa. This kind of circuit guarantees a strong mutualism but will also generate a kind of useless redundancy in which both A and B produce the same reward for the whole system.<br/> | ||
+ | Moreover this genetic circuit needs an initial external injection of or OXOC8 or OXOC12 to start the system.<br/> | ||
+ | Our feelings suggested us to keep Symbiome simple and more effective associating to each characters a specific and unique task.<br/> | ||
+ | On these new evidences we decided to give to cell A the role of starting up the system by constitutively producing OC12, leaving to cells B and C the production of the rewards, respectively the beta-glucosidase and the secreted beta-lactamase.<br/> | ||
</p></blockquote> | </p></blockquote> | ||
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+ | <div id="attributions" class="slide4 full-slides"> | ||
+ | <h1>ATTRIBUTIONS</h1> | ||
+ | <span style="text-align:center; display:block; font-size:14px;">This is a list of people who helped us making synbiome possible...</span><br/> | ||
+ | |||
+ | |||
+ | <table id="mytable"> | ||
+ | <thead> | ||
+ | |||
+ | <tr> | ||
+ | <th>Research Group</th> | ||
+ | <th>Who</th> | ||
+ | <th>What</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td class="logo"><a title="ICGEB" target="_blank" href="http://www.icgeb.org/mauro-giacca.html"><img src="https://static.igem.org/mediawiki/2011/a/ae/Units-attr-icgeb.jpg"></a></td> | ||
+ | <td> | ||
+ | <span class="attr-name"><b>Mauro Giacca,</b> Director, ICGEB Trieste Component, Group Leader</span><br/> | ||
+ | <span class="attr-descr">Molecular Medicine, ICGEB</span> | ||
+ | </td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"></td> | ||
+ | <td class="sottomesso"> | ||
+ | <span class="attr-name"><b>Miguel Mano,</b> Postdoc</span><br/> | ||
+ | <span class="attr-descr">Molecular Medicine, High Throughput Facility</span> | ||
+ | </td> | ||
+ | <td>Data acquisition</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"></td> | ||
+ | <td class="sottomesso"> | ||
+ | <span class="attr-name"><b>Bruna Marini,</b> PhD Student</span><br/> | ||
+ | <span class="attr-descr">Molecular Medicine</span> | ||
+ | </td> | ||
+ | <td>Microscopy</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"><a title="ICGEB" target="_blank" href="http://www.icgeb.org/vittorio-venturi.html"><img src="https://static.igem.org/mediawiki/2011/a/ae/Units-attr-icgeb.jpg"></a></td> | ||
+ | <td> | ||
+ | <span class="attr-name"><b>Vittorio Venturi,</b> Group Leader</span><br/> | ||
+ | <span class="attr-descr">Bacteriology, ICGEB</span> | ||
+ | </td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"></td> | ||
+ | <td class="sottomesso"> | ||
+ | <span class="attr-name"><b>Daniel Passos,</b> PhD Student</span><br/> | ||
+ | <span class="attr-descr">Bacteriology and plant bacteriology</span> | ||
+ | </td> | ||
+ | <td>Flow cytometry</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"><a title="ICGEB" target="_blank" href="http://www.icgeb.org/sandor-pongor.html"><img src="https://static.igem.org/mediawiki/2011/a/ae/Units-attr-icgeb.jpg"></a></td> | ||
+ | <td> | ||
+ | <span class="attr-name"><b>Sandor Pongor,</b> Group leader</span><br/> | ||
+ | <span class="attr-descr">Protein structure and bioinformatics, ICGEB</span> | ||
+ | </td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"><a title="BRC" target="_blank" href="http://www.szbk.u-szeged.hu/"><img src="https://static.igem.org/mediawiki/2011/0/0f/Units-attr-brc.jpg"/></a></td> | ||
+ | <td class="sottomesso"> | ||
+ | <span class="attr-name"><b>Adam Kerenyi,</b> Staff Scientist</span><br/> | ||
+ | <span class="attr-descr">Biological Research Center, Szeged, Hungary</span> | ||
+ | </td> | ||
+ | <td>Modeling</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"><a title="Dsv" target="_blank" href="http://dsv.units.it"><img src="https://static.igem.org/mediawiki/2011/f/fb/Units-logodsv.png"/></a></td> | ||
+ | <td> | ||
+ | <span class="attr-name"><b>Dr. Riccardo Sgarra</b></span><br/> | ||
+ | <span class="attr-descr">Department of Life Sciences, University of Trieste, Trieste, Italy</span> | ||
+ | </td> | ||
+ | <td>Mass Spectrometry</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"><a title="Edimburgh" target="_blank" href="http://www.ed.ac.uk/home"><img src="https://static.igem.org/mediawiki/2011/d/d0/Units-attr-edimburg.jpg"/></a></td> | ||
+ | <td> | ||
+ | <span class="attr-name"><b>Dr. C. French</b></span><br/> | ||
+ | <span class="attr-descr">The University of Edinburgh</span> | ||
+ | </td> | ||
+ | <td>BBa_K392008</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"><a title="D-BSSE" target="_blank" href="http://www.bsse.ethz.ch/"><img src="https://static.igem.org/mediawiki/2011/e/e2/Units-attr-dbsse.jpg"/></a></td> | ||
+ | <td> | ||
+ | <span class="attr-name"><b>Dr. M. Fussenegger</b></span><br/> | ||
+ | <span class="attr-descr">Department of Biosystems Science and Engineering</span> | ||
+ | </td> | ||
+ | <td>PWW0015</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="logo"><a title="IRBM" target="_blank" href="http://www.irbm.it/"><img src="https://static.igem.org/mediawiki/2011/3/32/Units-attr-irbm.jpg"/></a></td> | ||
+ | <td> | ||
+ | <span class="attr-name"><b>Dr. Cortese & P. Neddermann</b></span><br/> | ||
+ | <span class="attr-descr">IRBM Science Park</span> | ||
+ | </td> | ||
+ | <td>P65-TraR<br/>TraBOX-CMVmin</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="logo"><img src="https://static.igem.org/mediawiki/2011/8/81/Units-project.png"/></td> | ||
+ | <td> | ||
+ | <span class="attr-name"><b style="padding:0px;">Maddalena Fragnito</b></span> | ||
+ | </td> | ||
+ | <td><b><a title="Synbiome" target="_blank" href="http://www.youtube.com/watch?v=vJJEZTr9ZuM">Video editing</a></b></td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | <div id="cooperations" class="slide4 full-slides"> | ||
+ | <h1>COOPERATIONS</h1> | ||
+ | |||
+ | <div class="cooperations" style="background-image:url(https://static.igem.org/mediawiki/2011/0/08/Units-bkgY.png)" > | ||
+ | <span>Edinburgh IGEM team</span> | ||
+ | <div class="figure"><a href="https://2011.igem.org/Team:Edinburgh"><img style="padding-top:55px" width="200" src="https://static.igem.org/mediawiki/2011/d/d9/Edinburgh_home_logo.png"/></a></div> | ||
+ | <p>During the theoretical work on Synbiome we decided to use a β-glucosidase to provide our Interkingdom Consortium with a usable carbon source. We asked Chris French to send us the part BBa_K392008, made by team Osaka in 2010 and encoding a β-glucosidase from the bacterium Cellulomonas fimi. Firstly, we tested the part and it worked, but the DNA sequencing we decided to make during the generation of our parts BBa_K553005 and BBa_K553006 underlined the presence of a frameshift at the beginning of the coding sequence, introducing a stop codon. We shared this information with team Edinburgh and together we elaborated a solution to this strange problem (more details can be found on the cooperation page of team Edinburgh’s website). They were also very kind to provide us with a protocol of an assay that can be used to test the activity of this β-glucosidase using 4-methylumbelliferyl-β-D-glucuronide.</p></div> | ||
+ | |||
+ | <div class="cooperations"> | ||
+ | <span style="background-image:url(https://static.igem.org/mediawiki/2011/0/08/Units-bkgY.png)">Beta Glucosidase</span> | ||
+ | <div class="figure"> | ||
+ | <img width="200" src="https://static.igem.org/mediawiki/2011/a/a0/Units-cellobiosidase.jpg" alt="PDB ID code: 2O9R" title="PDB ID code: 2O9R"/> | ||
+ | </div><br/> | ||
+ | <span style="background-image:url(https://static.igem.org/mediawiki/2011/b/b2/Units-bkgR.png)">CYP Analysis</span> | ||
+ | <div class="figure"> | ||
+ | <img width="200" src="https://static.igem.org/mediawiki/2011/0/06/Units-cyp3.jpg"/> | ||
+ | </div> | ||
+ | <p></p></div> | ||
+ | |||
+ | <div class="cooperations" style="background-image:url(https://static.igem.org/mediawiki/2011/b/b2/Units-bkgR.png)" > | ||
+ | <span>Wageningen IGEM team</span> | ||
+ | <div class="figure"><a href="https://2011.igem.org/Team:Wageningen_UR"><img width="200" src="https://static.igem.org/mediawiki/2011/4/42/Wageningen_UR_logo.png"/></a></div> | ||
+ | <p>We also collaborated with team Wageningen, analyzing a bacterial strain expressing CFP with Fluorescence Microscopy. During the summer they typed on Twitter looking for a Confocal Microscope with the proper laser and filter for CFP’s wavelength. Since we had it here at ICGEB, we positively replied to team Wageningen’s request and helped them analyzing their samples, as they also reported in their website’s cooperation page.</p></div> | ||
+ | |||
+ | <div class="clear"></div> | ||
+ | </div><!-- #slide4 --> | ||
- | + | </div><!-- #container --> | |
</div><!-- .wrapper --> | </div><!-- .wrapper --> | ||
</div><!-- #main --> | </div><!-- #main --> |
Latest revision as of 14:51, 28 October 2011
SINBIOME ACKNOWLEDGEMENTS