Team:Peking S

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TO IGEM HQ!

A ‘Chemical Wire’ Toolbox for Synthetic Microbial Consortia

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Sponsed By

Click below To access a brief introduction of our project

Project presentation
Cell-cell communication-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient. Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
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-<font face="verdana "class="ws14">TO IGEM HQ!
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 <font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>A </span><span style="font-size:16px"><font color="#ffffff" class="ws16">&#8216;Chemical Wire&#8217; Toolbox for Synthetic Microbial Consortia</B></font></span></div>
+<div id="text3" style="position:absolute; overflow:hidden; left:160px; top:1070px; z-index:27;">
+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Q</span><span style="font-size:16px"><font color="#ffffff" class="ws16">uick Links</B></font></span></div>
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+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>S</span><span style="font-size:16px"><font color="#ffffff" class="ws16">ponsed By</B></font></span></div>
+<div id="text5" style="position:absolute; overflow:hidden; left:120px; top:750px;width:200px z-index:100;">
+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Click below</span><span style="font-size:16px"><font color="#000000" class="ws16"> To access a brief introduction of our project<br><br></B></font></span></div>
+<div id="text2" style="position:absolute; overflow:hidden;left:120px; top:440px; width:700px;z-index:1;">
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+<a href=http://sharesend.com/8nkvq>
+<b>Project presentation</b>
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 <b>Cell-cell communication</b>-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient.
-Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression system have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density have been created with supporting microfluid device.
+Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
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+<div id="image1" style="position:absolute; overflow:hidden; left:489px; top:1134px; width:292px; height:37px; z-index:40"><a href="http://www.phy.pku.edu.cn/English.html"><img src="https://static.igem.org/mediawiki/2011/7/7e/Pkuphy1.png"  alt="" title="" border=0 width=292 height=37></div>
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-<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Q</span><span style="font-size:16px"><font color="#ffffff" class="ws16">uick Links</B></font></span></div>
+<div id="image3" style="position:absolute; overflow:hidden; left:697px; top:1182px; width:194px; height:43px; z-index:42"><a href="http://dean.pku.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/2/20/PekingR_PKU_OFFICE_OF_EDUCATIONAL_ADMINISTRATION_%281%29.png" alt="" title="" border=0 width=194 height=43></div>
+<div id="image4" style="position:absolute; overflow:hidden; left:661px; top:1276px; width:255px; height:43px; z-index:43"><a href="http://www.bio.pku.edu.cn/english/"><img src="https://static.igem.org/mediawiki/2011/7/72/PekingR_PKU_SCHOOL_OF_LIFE_SCIENCE.png" alt="" title="" border=0 width=255 height=43></div>
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+<div id="image5" style="position:absolute; overflow:hidden; left:423px; top:1275px; width:232px; height:44px; z-index:44"><a href="http://www.bio.pku.edu.cn/syzx/">
-<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>S</span><span style="font-size:16px"><font color="#ffffff" class="ws16">ponsed By</B></font></span></div>
-<div id="text4" style="position:absolute; overflow:hidden; left:70px; top:150px;width:200px z-index:8;margin:30px 30px;">
-<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Click </span><span style="font-size:16px"><font color="#000000" class="ws16"> To access a brief introduction of our project<br><br></B></font></span></div>
-<div id="image1" style="position:absolute; overflow:hidden; left:489px; top:1084px; width:292px; height:37px; z-index:0"><a href="http://www.chem.pku.edu.cn/index.php?styleid=2"><img src="https://static.igem.org/mediawiki/2011/e/e3/PekingR_COLLEGE_OF_CHEMISTRY_AND_MOLECULAR_ENGINEERING.png"  alt="" title="" border=0 width=292 height=37></div>
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-<div id="image3" style="position:absolute; overflow:hidden; left:697px; top:1129px; width:194px; height:43px; z-index:2"><a href="http://dean.pku.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/2/20/PekingR_PKU_OFFICE_OF_EDUCATIONAL_ADMINISTRATION_%281%29.png" alt="" title="" border=0 width=194 height=43></div>
-<div id="image4" style="position:absolute; overflow:hidden; left:661px; top:1226px; width:255px; height:43px; z-index:3"><a href="http://www.bio.pku.edu.cn/english/"><img src="https://static.igem.org/mediawiki/2011/7/72/PekingR_PKU_SCHOOL_OF_LIFE_SCIENCE.png" alt="" title="" border=0 width=255 height=43></div>
-<div id="image5" style="position:absolute; overflow:hidden; left:423px; top:1225px; width:232px; height:44px; z-index:4"><a href="http://www.bio.pku.edu.cn/syzx/">
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