Team:Potsdam Bioware

From 2011.igem.org

(Difference between revisions)
 
(41 intermediate revisions not shown)
Line 2: Line 2:
<html>
<html>
-
<div class="div_home" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Project','_self');">
+
<div class="div_home" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Project/Summary','_self');">
-
<p>
+
 
 +
 
 +
<h2>Modification, Selection and Production of Cyclic Peptides for Therapy</h2>
 +
<table cellpadding="0"><td><img src="https://static.igem.org/mediawiki/2011/7/7d/UP_micrviridin_movie_final.gif"/></td>
 +
<td><p style="text-align:justify;">One key task of biopharmaceuticals is the binding and blocking of deregulated proteins. Towards this goal, we mutate and select microviridins, which are tricyclic depsipeptides from cyanobacteria. They are small but stable due to their post-translational side-chain crosslinking. Microviridins have a high potential for therapy as they can block disease-relevant proteases. Yet, the possibilities of cyclic peptides are largely untapped since genetic systems for optimization are not well established. Thus, we developed synthetic systems for the mutation, selection and production of such peptides. We utilized the 6.5 kb microviridin (<i>mdn</i>) gene cluster cloned in <i>E. coli</i> plasmids, established random mutagenesis and generated focused libraries of microviridins. For selection against a panel of proteases, we are applying and testing phage display, and we are constructing a novel in-vivo selection device, which links protease blocking to antibiotic resistance. Our systems adhere to the BioBrick standards. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Project/Summary"><span class="bold">[more]</span></a></p>
 +
</td></table>
-
<h2>Project</h2>
 
-
<img class="right" src="https://static.igem.org/mediawiki/2011/e/e0/UP_zahnrad_blau_small.png"/>
 
-
<p style="text-align:justify;">One key task of biopharmaceuticals is the binding and blocking of deregulated proteins. Towards this goal, we mutate and select microviridins, which are tricyclic depsipeptides from cyanobacteria. They are small but stable due to their post-translational side-chain crosslinking. Microviridins have a high potential for therapy as they can block disease-relevant proteases. Yet, the possibilities of cyclic peptides are largely untapped since genetic systems for optimization are not well established. Thus, we developed synthetic systems for the mutation, selection and production of such peptides. We use the 6.5 kb microviridin (mdn) gene cluster cloned in E. coli plasmids, established random mutagenesis and generated focused libraries of microviridins. For selection against a panel of proteases, we are applying and testing phage display, and we are constructing a novel in-vivo selection device, which links protease blocking to antibiotic resistance. Our systems, including the 6.5 kb cluster, adhere to the BioBrick standards.
 
-
</p>
 
-
</p>
 
</div>
</div>
<!---Box on the upper left: Software--->
<!---Box on the upper left: Software--->
<div class="box_home" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Software','_self');">
<div class="box_home" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Software','_self');">
 +
<img id="topRight" src="https://static.igem.org/mediawiki/2011/b/bb/UP_home_Software1.png">
<h2>Software</h2>
<h2>Software</h2>
<p>
<p>
Line 35: Line 36:
<div class="box_home box_long_news news_ticker" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Team','_self');">
<div class="box_home box_long_news news_ticker" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Team','_self');">
-
<a href="https://2011.igem.org/Team:Potsdam_Bioware/NoteBook/Statistics" style="z-index:20;position:relative;"><h2>Team</h2></a>
+
<a href="https://2011.igem.org/Team:Potsdam_Bioware/Team" style="z-index:20;position:relative;"><h2>Team</h2></a>
<ul id="news" class="newsticker">
<ul id="news" class="newsticker">
<li><div class="news">
<li><div class="news">
Line 106: Line 107:
</li>
</li>
<li><div class="news">
<li><div class="news">
-
      
+
     <img src="https://static.igem.org/mediawiki/2011/8/87/UP_Paul.jpg" alt="Paul Kaufmann" />
     <p>Paul Kaufmann</p>
     <p>Paul Kaufmann</p>
</div>
</div>
Line 141: Line 142:
</li>
</li>
<li><div class="news">
<li><div class="news">
 +
<img src="https://static.igem.org/mediawiki/2011/c/c0/UP_Oliver.jpg" alt="Oliver Zimmer" />
     <p>Oliver Zimmer</p>
     <p>Oliver Zimmer</p>
</div>
</div>
Line 158: Line 160:
<!---Box on the upper right: BioBricks--->
<!---Box on the upper right: BioBricks--->
<div height="290px">
<div height="290px">
-
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/BioBricks','_self');">
+
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Data_Page','_self');">
-
<img class="right" src="https://static.igem.org/mediawiki/2011/e/e0/UP_zahnrad_blau_small.png"/>
+
<img class="right" src="https://static.igem.org/mediawiki/2011/8/84/UP_home_BioBricks.png"/>
<h2>BioBricks</h2>
<h2>BioBricks</h2>
-
<p class="standard">Yes, we have some… check it out! :D <a href="https://2011.igem.org/Team:Potsdam_Bioware/BioBricks"><span class="bold">[more]</a></span></p>
+
<p class="standard">
 +
Our datapage displays our systems and BioBricks in a nutshell. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Data_Page"><span class="bold">[more]</a></span><br> <br>
 +
Our BioBricks are top-down for the <i>mdn</i> gene cluster and botton-up for detection and selection. <a href="https://2011.igem.org/Team:Potsdam_Bioware/BioBricks"><span class="bold">[more]</a></span></p>
</div>
</div>
<!---Box on the lower left: Human Practice--->
<!---Box on the lower left: Human Practice--->
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Safety_Ethics','_self');">
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Safety_Ethics','_self');">
-
<img class="right" src="https://static.igem.org/mediawiki/2011/e/e0/UP_zahnrad_blau_small.png"/>
+
<img class="right" src="https://static.igem.org/mediawiki/2011/1/19/UP_home_Safety.png"/>
<h2>Safety & Ethics</h2></a>
<h2>Safety & Ethics</h2></a>
-
<p class="standard">We communicated safety issues and ethic controversies in seminars and polled all members of the German parliament for their opininon on synthetic biology. Learn more about German views. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Safety_Ethics"><span class="bold">[more]</span></a></p>
+
<p class="standard">We discussed safety issues and ethic controversies in seminars and polled all members of the German parliament for their opinion on synthetic biology. Learn more about German views. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Safety_Ethics"><span class="bold">[more]</span></a></p>
</div>
</div>
<!---Box on the lower right: labjournal--->
<!---Box on the lower right: labjournal--->
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Labjournal','_self');">
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Labjournal','_self');">
-
<img class="right" src="https://static.igem.org/mediawiki/2011/e/e0/UP_zahnrad_blau_small.png"/>
+
<img class="right" src="https://static.igem.org/mediawiki/2011/2/2a/UP_home_Labjournal.png"/>
<h2>Labjournal</h2>
<h2>Labjournal</h2>
<p>
<p>
-
All the hours spent in the lab - thinking, laughing, sweating and hoping. Here is the guide through our lab work.<a href="https://2011.igem.org/Team:Potsdam_Bioware/journal/journal"><span class="bold">[more]</span></a></p>
+
All the hours spent in the lab - thinking, laughing, sweating and hoping. Here is the tour guide through our lab work. We hope, you enjoy the trip. Please fasten your seat belt and mind the gap. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Labjournal"><span class="bold">[more]</span></a></p>
</div>
</div>
-
<!---Box on the lower right: Modelling--->
+
<!---Box on the lower right: Modeling--->
-
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Project/Details#Modelling','_self');">
+
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Project/Summary#Modeling','_self');">
-
<img class="right" src="https://static.igem.org/mediawiki/2011/e/e0/UP_zahnrad_blau_small.png"/>
+
<img class="right" src="https://static.igem.org/mediawiki/2011/b/bd/UP_home_Modeling.png"/>
-
<h2>Modelling</h2></a>
+
<h2>Modeling</h2></a>
-
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua.
+
<p class="standard">
-
<a href="https://2011.igem.org/Team:Potsdam_Bioware/software"><span class="bold">[more]</span></a></p>
+
This year we focused on systems modeling in which the reaction kinetics of the <i>in vivo</i> selection are analyzed. Read more about the predictions we were able to derive from our model.
 +
<a href="https://2011.igem.org/Team:Potsdam_Bioware/Project/Summary#Modeling"><span class="bold">[more]</span></a></p>
</div>
</div>
</div>
</div>
Line 203: Line 208:
-
<html><center><div id="clustrmaps-widget"></div><script type="text/javascript">var _clustrmaps = {'url' : 'https://2011.igem.org/Team:Potsdam_Bioware', 'user' : 910340, 'server' : '2', 'id' : 'clustrmaps-widget', 'version' : 1, 'date' : '2011-07-15', 'lang' : 'en', 'corners' : 'square' };(function (){ var s = document.createElement('script'); s.type = 'text/javascript'; s.async = true; s.src = 'http://www2.clustrmaps.com/counter/map.js'; var x = document.getElementsByTagName('script')[0]; x.parentNode.insertBefore(s, x);})();</script><noscript><a href="http://www2.clustrmaps.com/user/239de404"><img src="http://www2.clustrmaps.com/stats/maps-no_clusters/2011.igem.org-Team-Potsdam_Bioware-thumb.jpg" alt="Locations of visitors to this page" /></a></noscript><center></html>
+
<html><center>
-
 
+
<div id="clustrmaps-widget"></div><script type="text/javascript">var _clustrmaps = {'url' : 'https://2011.igem.org/Team:Potsdam_Bioware', 'user' : 910340, 'server' : '2', 'id' : 'clustrmaps-widget', 'version' : 1, 'date' : '2011-07-15', 'lang' : 'en', 'corners' : 'square' };(function (){ var s = document.createElement('script'); s.type = 'text/javascript'; s.async = true; s.src = 'http://www2.clustrmaps.com/counter/map.js'; var x = document.getElementsByTagName('script')[0]; x.parentNode.insertBefore(s, x);})();</script><noscript><a href="http://www2.clustrmaps.com/user/239de404"><img src="http://www2.clustrmaps.com/stats/maps-no_clusters/2011.igem.org-Team-Potsdam_Bioware-thumb.jpg" alt="Locations of visitors to this page" /></a></noscript>
-
 
+
-
 
+
-
 
+
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert ***
+
-
 
+
-
<html>
+
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
 
+
-
</div>
+
-
</div>
+
-
 
+
-
 
+
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace.
+
-
 
+
-
{|align="justify"
+
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
-
|[[Image:Potsdam_Bioware_logo.png|200px|right|frame]]
+
-
|-
+
-
|
+
-
''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
-
|[[Image:Potsdam_Bioware_team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:Potsdam_Bioware | Team Example]]
+
-
|}
+
-
 
+
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<p style="font-size:xx-small; text-align:center; color:grey">primary contact: Kristian Müller, kristian@syntbio.net, http://www.syntbio.net</p>
-
!align="center"|[[Team:Potsdam_Bioware|Home]]
+
</center></html>
-
!align="center"|[[Team:Potsdam_Bioware/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Potsdam_Bioware Official Team Profile]
+
-
!align="center"|[[Team:Potsdam_Bioware/Project|Project]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Modeling|Modeling]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Notebook|Notebook]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Safety|Safety]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Attributions|Attributions]]
+
-
|}
+
-
</html>  -->
+

Latest revision as of 13:38, 16 November 2011

Modification, Selection and Production of Cyclic Peptides for Therapy

One key task of biopharmaceuticals is the binding and blocking of deregulated proteins. Towards this goal, we mutate and select microviridins, which are tricyclic depsipeptides from cyanobacteria. They are small but stable due to their post-translational side-chain crosslinking. Microviridins have a high potential for therapy as they can block disease-relevant proteases. Yet, the possibilities of cyclic peptides are largely untapped since genetic systems for optimization are not well established. Thus, we developed synthetic systems for the mutation, selection and production of such peptides. We utilized the 6.5 kb microviridin (mdn) gene cluster cloned in E. coli plasmids, established random mutagenesis and generated focused libraries of microviridins. For selection against a panel of proteases, we are applying and testing phage display, and we are constructing a novel in-vivo selection device, which links protease blocking to antibiotic resistance. Our systems adhere to the BioBrick standards. [more]

Software

BioLog – The new allround talent in the lab for your smart phone! Our app combines several features frequently used in the lab. The software stores basic protocols in your pocket - easy, handy and ready to use… [more]

Team
  • Nicole Albrecht

    Nicole Albrecht

  • Katharina Berger

    Katharina Berger

  • Nadja Bjelopoljak

    Nadja Bjelopoljak

  • Nadine Boehmer

    Nadine Boehmer

  • Vanessa Boehmer

    Vanessa Boehmer

  • Jessica Eger

    Jessica Eger

  • Steffi Sempert

    Steffi Sempert

  • Niels Weisbach

    Niels Weisbach

  • Sebastian Hanke

    Sebastian Hanke

  • Sascha Ramm

    Sascha Ramm

  • Paul Kaufmann

    Paul Kaufmann

  • Stefan Wahlefeld

    Stefan Wahlefeld

  • Sandrina Heyde

    Sandrina Heyde

  • Sabine Meyer

    Sabine Meyer

  • Niklas Laasch

    Niklas Laasch

  • Oliver Zimmer

    Oliver Zimmer

  • Tobias Wenzel

    Tobias Wenzel

BioBricks

Our datapage displays our systems and BioBricks in a nutshell. [more]

Our BioBricks are top-down for the mdn gene cluster and botton-up for detection and selection. [more]

Safety & Ethics

We discussed safety issues and ethic controversies in seminars and polled all members of the German parliament for their opinion on synthetic biology. Learn more about German views. [more]

Labjournal

All the hours spent in the lab - thinking, laughing, sweating and hoping. Here is the tour guide through our lab work. We hope, you enjoy the trip. Please fasten your seat belt and mind the gap. [more]

Modeling

This year we focused on systems modeling in which the reaction kinetics of the in vivo selection are analyzed. Read more about the predictions we were able to derive from our model. [more]


primary contact: Kristian Müller, kristian@syntbio.net, http://www.syntbio.net

Retrieved from "http://2011.igem.org/Team:Potsdam_Bioware"