Team:Wisconsin-Madison/platereader

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Latest revision as of 23:00, 28 September 2011

Project >> Overview, Ethanol Sensor, Alkane Sensor, Microcompartment

Plate Reader (Fluorescence Detection)

To measure the fluorescence of our biosensor, we are using a Tecan ® Infinite™ Microplate Reader on 96 well plates. The outer ring of the plate is filled with a blank solution, typically the growth media used for the sensor, to prevent evaporation of the other samples in the plate. Each plate reader experiment is run to keep the optical densities (ODs) of each well as similar as possible, however, the OD is corrected later in the analysis of the data. Each row on the plate is a different sample, and each was run in triplicate. Plate reader protocols may be found in the protocols section.

Learn more about biosensors.

About the image: Typical plate reader setup to test the efficacy of our EtOH sensor. Each lane is a sample, which was run in triplicate. The outer ring is blank media to prevent evaporation of the center samples.

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 		<a href="https://2011.igem.org/Team:Wisconsin-Madison">Main</a>
 		<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
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                  <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">Directed Evolution</a>
 		<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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 		<a href="https://2011.igem.org/Team:Wisconsin-Madison/protocols">Protocols</a>
-		<a href="https://2011.igem.org/Team:Wisconsin-Madison/calender">Calender</a>
+		<a href="https://2011.igem.org/Team:Wisconsin-Madison/calender">Calendar</a>
 		<a href="https://2011.igem.org/Team:Wisconsin-Madison/references">References</a>
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 		<a href="https://2011.igem.org/Team:Wisconsin-Madison/reuposterSession">REU Poster Session</a>
 		<a href="https://2011.igem.org/Team:Wisconsin-Madison/socialmedia">Social Media</a>
-		<a href="https://2011.igem.org/Team:Wisconsin-Madison/presentations">Presentations</a>
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+		<a href="https://2011.igem.org/Team:Wisconsin-Madison/humanpractice">Human Practice</a>
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 <table border="0" cellspacing="4" cellpadding="0"><tr><td>
-<img src="https://static.igem.org/mediawiki/2011/8/8d/Bmc.png" width = "360" align="right"/>
+<img src="https://static.igem.org/mediawiki/2011/f/fe/Plat_reader.jpg" width = "400" align="right"/>
 <strong><font size="3">
-Bacterial Microcompartment
+Plate Reader (Fluorescence Detection)
 </font></strong><p>
-Bacterial microcompartments (BMC) are tiny protein-assembled compartments which are newly discovered in many bacteria strains. Their existence in cells enables bacteria to compact certain reactions within small defined space such that the enzyme efficiency can be increased due to folded substrate concentration and also those generated mediates from toxic reactions are separated from the rest of cell contains. Pdu microcompartment, which was originally found in Salmonella enteric and Citrobactor freundii, encloses metabolisms that utilize propanediol as a carbon resource. Literatures and former USTC iGEM 2010 team showed that empty microcompartments can be successfully assembled in E. coli and some genes are suggested to be able to direct proteins to outer surface or inner parts of the microcompartments respectively. Based on these findings, we UW-Madison 2011 iGEM team believe that BMC has the potentiality to dramatically improve bacteria production efficiency and can be further remodeled to conduct new functions. And so far, we are trying to assemble pdu microcompartments in E.coli, characterize the assembly rates and make E.coli produce biofuels such as butanol and fatty acids.
+To measure the fluorescence of our biosensor, we are using a Tecan ® Infinite™ Microplate Reader on 96 well plates. The outer ring of the plate is filled with a blank solution, typically the growth media used for the sensor, to prevent evaporation of the other samples in the plate. Each plate reader experiment is run to keep the optical densities (ODs) of each well as similar as possible, however, the OD is corrected later in the analysis of the data. Each row on the plate is a different sample, and each was run in triplicate. Plate reader protocols may be found in the <a href="https://2011.igem.org/Team:Wisconsin-Madison/protocols">protocols</a> section.
 <p><br>
-Learn more about <a href="https://2011.igem.org/Team:Wisconsin-Madison/biofuels">biofuels</a>.
+Learn more about <a href="https://2011.igem.org/Team:Wisconsin-Madison/biosensor">biosensors</a>.
 <p>
-<font size="1"><i>Image Source: sddddddddddddddddddddddddddddddddddd</i></font>
+<font size="1"><i>About the image: Typical plate reader setup to test the efficacy of our EtOH sensor. Each lane is a sample, which was run in triplicate. The outer ring is blank media to prevent evaporation of the center samples.</i></font>