Team:Freiburg/Notebook/8 August

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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 8 August </a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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===NAME OF YOUR EXPERIMENT===
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===Lysis + RBS===
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'''Investigators:NAME'''
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'''Investigators:Theo'''
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Sent samples for sequencing, Testdigestions of whole temperature sensitive cassette showed no correct inserts! (:()
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==

Latest revision as of 01:06, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

green light receptor

1. Quickchange


Investigator:Jakob


Name:

Jakob

Date:

08.08.2011

Continue from Experiment:
Project Name:

Quickchange CcaR and CcaS

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20
10µl 5x Phusion Buffer
2.5µl Primer fw Primer: P12(CcaR) and P14(CcaS
2.5µl Primer dw Primer: P13(CcaR) and P15(CcaS)
1µl dNTPs
1µl DNA-Template DNA: CcaR and CcaS
0.5 µl Phusion (add in the end)



2. Digest with DpnI

  • Digest of the quickchange above

3. Transformation


  • Transformation from digest above


  • Note: I'm such a fool...forgot the gel...

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

Lysis + RBS

Investigators:Theo

Sent samples for sequencing, Testdigestions of whole temperature sensitive cassette showed no correct inserts! (:()

Precipitator

NAME OF YOUR EXPERIMENT: Cloning

Investigators: Sophie
continue from experiment: miniprep (the, 8.8.)
Project name: new 3A assembly with amp vector
Parts: psB1A3 (Eco & Pst)
GFP-pbd ( Xba & Pst)
PR4 or PR6 (Eco & Spe)
Why? The last cloning of Theo and Jakob produced one clone with GFP-pbd inside but without PR. So I'm trying again to clone with two different PRs with different RBS strenghts.
Stored in: "Minipreps, verdaut"-box

                       

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