From 2011.igem.org
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| - | == PCR product purification ==
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| - | # Mix 1 volume of sample with 2 volumes of NT buffer in an 1,5 ml Eppendorf tube.
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| - | # Place a column into a 2 ml collection tube and load the sample,
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| - | # Centrifuge at 11.000 g for 1 min.
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| - | # Discard flow through and place the column back into the collection tube.
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| - | # Add 600 µl NT3 buffer and centrifuge at 11.000 g for 1 min.
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| - | # Discard flow through and place the column back into the collection tube.
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| - | # Centrifuge at 11.000 g for 2 min to remove NT3 buffer. Discard flow through.
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| - | # Place the column into a clean 1,5 ml Eppendorf tube.
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| - | # Add 30 µl Elution Buffer NE and incubate at RT for 1 min to increase the yield of eluted DNA.
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| - | # Centrifuge at 11.000 g for 1 min.
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Latest revision as of 19:10, 21 September 2011
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