Team:UNAM-Genomics Mexico/Project

From 2011.igem.org

(Difference between revisions)
m (version 1.0.4)
 
(50 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:UNAM-Genomics Mexico/Templates/borrando}}
+
{{:Team:UNAM-Genomics Mexico/Templates/Modeling| content=
-
{{:Team:UNAM-Genomics Mexico/Templates/Project}}
+
__NOTOC__
-
{{:Team:UNAM-Genomics Mexico/Templates/Menu}}
+
-
<html xmlns="http://www.w3.org/1999/xhtml">
+
=Project=
-
<!--
 
-
@VERSION 1.0.4
 
-
@NAME    Project page for the UNAM-Genomics_Mexico team wiki.
 
-
-->
 
-
<head>
 
-
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
 
-
<link href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Templates/Home" rel="stylesheet" type="text/css" />
 
-
<title>UNAM-Genomics_Mexico</title>
 
-
<script type="text/javascript">
 
-
  var _gaq = _gaq || [];
+
Among the biological systems that produce hydrogen, the most efficient ones achieve it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium ''Rhizobium etli'', during its symbiotic relationship with the common bean ''Phaseolus vulgaris'', can transform nitrogen gas into ammonia in a process called nitrogen fixation. In exchange the plant provides the bacteria with carbon sources and a protected niche inside its root, where ''Rhizobium etli'' reaches a hypoxic state. We will exploit this microenvironment to produce hydrogen in ''Rhizobium etli'' introducing a pathway assembled with elements from ''Clostridium acetobutylicum'', ''Desulfovibrio africanus'' and ''Chlamydomonas reinhardtii'', while maintaining nitrogen fixation. The two goals of our project are to make ''Rhizobium etli'' a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials.
-
  _gaq.push(['_setAccount', 'UA-19624614-2']);
+
-
  _gaq.push(['_trackPageview']);
+
-
  (function() {
 
-
    var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true;
 
-
    ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js';
 
-
    var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s);
 
-
  })();
 
-
</script>
+
----
-
</head>
+
-
<body>
+
<html>
-
<div id="container">
+
<div class="top-slider-main">
-
<div class="header">
+
    <div class="slider-box1">
-
<img src="https://static.igem.org/mediawiki/2011/5/5e/Header.jpg" width="964px" height="203px" id="Insert_logo" style="background: #8090AB; display:block;"></a>  
+
        <div class="left"><br /><center></center></div>
 +
      <div class="content"><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Notebook/SA"><img src="https://static.igem.org/mediawiki/2011/8/8a/Unamgenomicsassembly.jpg"></a></div>
 +
    </div>
 +
    <div class="slider-box2">
 +
        <div class="left"><br /><center></center></div>
 +
        <div class="content" valign="middle"><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Project/HydrogenProduction"><img src="https://static.igem.org/mediawiki/2011/0/00/Unamgenomicsrsz_operons.jpg"></a></div>
 +
    </div>
 +
    <div class="slider-box3">
 +
        <div class="left"><br /><center></center></div>
 +
        <div class="content"><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Project/RhizobialKit"><img src="https://static.igem.org/mediawiki/2011/e/ea/Unamgenomicsrsz_pberc5.jpg"></a></div>
 +
    </div>
 +
    <div class="slider-box4">
 +
        <div class="left"><br /><center></center></div>
 +
        <div class="content"><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Notebook/repC"><img src="https://static.igem.org/mediawiki/2011/8/82/UNAM_Genomics_MexicoDuplicadoparawiki.jpg"></a></div>
 +
    </div>
 +
    <div class="slider-box5">
 +
        <div class="left"><br /><center></center></div>
 +
        <div class="content"><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Team"><img src="https://static.igem.org/mediawiki/2011/3/3e/Unamgenomics6.jpg"></a></div>
 +
    </div>
 +
    <div class="slider-box6">
 +
<div class="left"><br /><center></center></div>
 +
        <div class="content"><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Project"><img src="https://static.igem.org/mediawiki/2011/1/1f/Unamgenomicsproject1.jpg"></a></div>
 +
    </div>
 +
</div>
-
<!-- Menu Code -->
+
</html>
-
<table width="100%" border="0" class="header1">
+
-
<tr><td align="center" valign="center">
+
-
<td width="964px">
+
-
<ul id="h2igemmenu">
+
-
+
-
<li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico">H2 Homepage</a></li>
+
-
<li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
-
<ul class="istylem0" style="width:195px;">
+
-
<li><a href="#"><span>Intro</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Theory</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Description</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Aplications</span><![if gt IE 6]></a>
+
-
<![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
+
-
</ul>
+
-
<li class="istylei0"><a class="istylei0" href="#">Abstract in a Picture</a></li>
+
-
<li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Team"><span>Team Members</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
-
<ul class="istylem0" style="width:195px;">
+
-
<li><a href="https://igem.org/Team.cgi?year=2011&team_name=UNAM-Genomics_Mexico"><span>Oficial Team Profile</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Motivation</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Brainstorm</span><![if gt IE 6]></a>
+
-
<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Team"><span>Team Members</span><![if gt IE 6]></a>
+
-
<![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
-
<ul class="istylem0" style="width:173px;">
+
-
<li><a href="https://2011.igem.org/User:Jurquiza">Uriel</a></li>
+
-
<li><a href="https://2011.igem.org/User:Nandomgu">Nando</a></li>
+
-
<li><a href="#">Paz</a></li>
+
-
<li><a href="https://2011.igem.org/User:Fibonacci_Lopez">Fabricio</a></li>
+
-
<li><a href="https://2011.igem.org/User:Hmedina">Hector</a></li>
+
-
<li><a href="https://2011.igem.org/User:Dgarcia">Daniela</a></li>
+
-
<li><a href="https://2011.igem.org/User:Khronus">Daniel</a></li>
+
-
<li><a href="https://2011.igem.org/User:Melissam">Melissita</a></li>
+
-
<li><a href="https://2011.igem.org/User:Alecanda">Aaron</a></li>
+
-
<li><a href="https://2011.igem.org/User:Atrevino">Abiel</a></li>
+
-
<li><a href="https://2011.igem.org/User:Meli_arr">Meli</a></li>
+
-
<li><a href="https://2011.igem.org/User:Paedugar">Pae</a></li>
+
-
<li><a href="https://2011.igem.org/User:Vladimir">Vladimir</a></li>
+
-
<li><a href="https://2011.igem.org/User:Gustavoruiz">Gustavo</a></li>
+
-
<li><a href="https://2011.igem.org/User:Palatris">Pato</a></li>
+
-
<li><a href="https://2011.igem.org/User:Hamarillo">Helena</a></li>
+
-
<li><a href="https://2011.igem.org/User:Paxiveri">Paloma</a></li>
+
-
<li><a href="https://2011.igem.org/User:Nalvarez">Nataly</a></li>
+
-
<li><a href="https://2011.igem.org/User:Misticastillo">Misti</a></li>
+
-
<li><a href="https://2011.igem.org/User:AraceliAM">Araceli</a></li>
+
-
<li><a href="https://2011.igem.org/User:Ocarrillo">Omar</a></li>
+
-
<li><a href="https://2011.igem.org/User:MarioV">Mario</a></li>
+
-
</ul>
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
+
-
</ul>
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
+
-
<li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Notebook"><span>Notebook</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
-
<ul class="istylem0" style="width:195px;">
+
-
<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Parts"><span>Parts</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Calendar</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Protocols</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Programs</span><![if gt IE 6]></a>
+
-
<li><a href="#"><span>Wet-Lab</span><![if gt IE 6]></a>
+
-
<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Modeling"><span>Modeling</span><![if gt IE 6]></a>
+
-
<![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
+
-
</ul>
+
-
<li class="istylei0"><a class="istylei0" href="#"><span>Modeling</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--></li>
+
-
<li class="istylei0"><a class="istylei0" href="#"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
-
<ul class="istylem0" style="width:195px;">
+
-
<li><a href="#"><span>Ethics</span><![if gt IE 6]></a>
+
-
<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Safety"><span>Biosecurity-Biosafety</span><![if gt IE 6]></a>
+
-
<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Bio-SintetizArte"><span>BioArt</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
+
-
<ul class="istylem0" style="width:173px;">
+
-
<li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Bio-SintetizArte">Call</a></li>
+
-
<li><a href="#">Photos</a></li>
+
-
<li><a href="#">Send a Phrase</a></li>
+
-
</ul>
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
</ul></li>
+
-
<li class="istylei0"><a class="istylei0" href="#">Sponsors</a></li>
+
-
</ul>
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
+
-
</td>
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
<!-- End: Menu code -->
+
-
</div><!--class="header"-->
+
-
<!-- Page Body Code. -->
+
 
-
<div class="left_image">
+
 
-
<img class="sub_project" alt="Our Project Image" src="https://static.igem.org/mediawiki/2011/d/dd/GPPU_in_the_lab_500x500.jpg" title="Wet lab">
+
}}
-
</div>
+
-
<div class="summary_text">
+
-
<p><a class="project"><i>Rhizobium etli</i></a> is a bacterial species that can live either freely or in symbiosis with the bean <a class="project"><i>Phaseolus vulgaris</i></a>.
+
-
In it's symbiotic stage <i>R. etli</i> lives in nodules in the plant's root and has the ability to <a class="project">fixate nitrogen</a> gas as amine molecules.
+
-
Inside the nodule the environment is <a class="project">hypoxigenic</a>.
+
-
The <a class="project">enzymatic production of hydrogen</a> requires a hypoxigenic environment to favor the reactions.
+
-
We want to introduce the metabolic pathway of hydrogen production into <i>R. etli</i> and take advantage of its niche in the hypoxic nodule.
+
-
Our goal is <a class="project">hydrogen</a> production in the <i>Phaseolus vulgaris</i> root.</p>
+
-
</div>
+
-
<div class="descriptive_text">
+
-
<p>These are the goals in our project:</p>
+
-
<h1 class="project_goals">System design</h1>
+
-
<h1 class="project_goals">System insertion into <i>E. coli</i></h1>
+
-
<p><i>Rhizobium etli</i> can't be transformed, and it's phages haven't been studied enough.
+
-
However <i>R. etli</i> can receive DNA if conjugated with <i>Escherichia coli</i>.
+
-
To introduce our system into <i>R. etli</i> we must first introduce it into <i>E. coli</i>.</p>
+
-
<h1 class="project_goals">System conjugation into <i>R. etli</i></h1>
+
-
<h1 class="project_goals">Bean innoculation</h1>
+
-
</div>
+
-
</div><!--id="container"-->
+
-
</body>
+
-
</html>
+

Latest revision as of 03:04, 29 September 2011

UNAM-Genomics_Mexico


Project

Among the biological systems that produce hydrogen, the most efficient ones achieve it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium Rhizobium etli, during its symbiotic relationship with the common bean Phaseolus vulgaris, can transform nitrogen gas into ammonia in a process called nitrogen fixation. In exchange the plant provides the bacteria with carbon sources and a protected niche inside its root, where Rhizobium etli reaches a hypoxic state. We will exploit this microenvironment to produce hydrogen in Rhizobium etli introducing a pathway assembled with elements from Clostridium acetobutylicum, Desulfovibrio africanus and Chlamydomonas reinhardtii, while maintaining nitrogen fixation. The two goals of our project are to make Rhizobium etli a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials.








Retrieved from "http://2011.igem.org/Team:UNAM-Genomics_Mexico/Project"