Team:UNAM-Genomics Mexico/Project

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Among the biological systems that produce hydrogen, the most efficient ones achieve it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium ''Rhizobium etli'', during its symbiotic relationship with the common bean ''Phaseolus vulgaris'', can transform nitrogen gas into ammonia in a process called nitrogen fixation. In exchange the plant provides the bacteria with carbon sources and a protected niche inside its root, where ''Rhizobium etli'' reaches a hypoxic state. We will exploit this microenvironment to produce hydrogen in ''Rhizobium etli'' introducing a pathway assembled with elements from ''Clostridium acetobutylicum'', ''Desulfovibrio africanus'' and ''Chlamydomonas reinhardtii'', while maintaining nitrogen fixation. The two goals of our project are to make ''Rhizobium etli'' a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials.
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This page is still under construction, please follow this link to view the <a class="HP" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Bio-SintetizArte">Biosintetizarte</a> page (in Spanish).
 
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<a class="project"><i>Rhizobium etli</i></a> is a bacterial species that can live either freely or in symbiosis with the bean <a class="project"><i>Phaseolus vulgaris</i></a>.
 
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In it's symbiotic stage <i>R. etli</i> lives in nodules in the plant's root and has the ability to <a class="project">fixate nitrogen</a> gas as amine molecules.
 
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Inside the nodule the environment is <a class="project">hypoxigenic</a>.
 
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The <a class="project">enzymatic production of hydrogen</a> requires a hypoxigenic environment to favor the reactions.
 
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We want to introduce the metabolic pathway of hydrogen production into <i>R. etli</i> and take advantage of its niche in the hypoxic nodule.
 
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Our goal is <a class="project">hydrogen</a> production in the <i>Phaseolus vulgaris</i> root.
 
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These are the goals in our project:
 
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<h1 class="project_goals">System design</h1>
 
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<h1 class="project_goals">System insertion into <i>E. coli</i></h1>
 
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<i>Rhizobium etli</i> can't be transformed, and it's phages haven't been studied enough.
 
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However <i>R. etli</i> can receive DNA if conjugated with <i>Escherichia coli</i>.
 
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To introduce our system into <i>R. etli</i> we must first introduce it into <i>E. coli</i>.
 
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<h1 class="project_goals">System conjugation into <i>R. etli</i></h1>
 
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<h1 class="project_goals">Bean innoculation</h1>
 
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Latest revision as of 03:04, 29 September 2011

UNAM-Genomics_Mexico


Project

Among the biological systems that produce hydrogen, the most efficient ones achieve it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium Rhizobium etli, during its symbiotic relationship with the common bean Phaseolus vulgaris, can transform nitrogen gas into ammonia in a process called nitrogen fixation. In exchange the plant provides the bacteria with carbon sources and a protected niche inside its root, where Rhizobium etli reaches a hypoxic state. We will exploit this microenvironment to produce hydrogen in Rhizobium etli introducing a pathway assembled with elements from Clostridium acetobutylicum, Desulfovibrio africanus and Chlamydomonas reinhardtii, while maintaining nitrogen fixation. The two goals of our project are to make Rhizobium etli a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials.








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