Team:DTU-Denmark-2

From 2011.igem.org

(Difference between revisions)

Latest revision as of 23:10, 21 September 2011

Making Molecular Biology Easier

We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and Aspergilli ready to plug 'n' play. You can find more information about how this standard works here.

A Reporter System

As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism Aspergillus nidulans and the mammalian cell line U-2 OS with great success.

Flexibility

Standardization entails rigidity. Therefore we have written a guide that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.

Applications

Here you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.

Data Page

You can find an overview of our project on our Data Page. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.

Achievements

Designing a novel assembly standard and creating a reporter system for Aspergillus nidulans and mammalian cells are our main achievements. You can read more about our accomplishments on this page.

The Team

We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our Team Page.

iGEM 2011 Wiki Main Page

Sponsored by

Comments or questions to the team? Please Email us

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 <div class="red">
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+<p align="justify">
-Our mission
+<font color="#000000" face="arial" size="5" >
-</font>
+<b>Making Molecular Biology Easier</b>
-<br>
+</font></p>
+<font color="#000000" face="arial" size="2" >
+<p align="justify">
 <br>
+<p align="justify">
+<b>We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and <i>Aspergilli </i> ready to plug 'n' play. You can find more information about how this standard works <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly">here</a>.<b>
+</p>
+</font>
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+ <br>
-insert text here!
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+<b>A Reporter System</b><br><br>
+</font>
+<div class="image2">
-<br>
-<br>
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-<div class="green">
-<br>
+<font color="#000000" face="arial" size="1" >
-<br>
+<p align="justify">
-<br>
+As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi" ><i>Aspergillus nidulans</i></a> and the <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/mammalian"> mammalian cell line U-2 OS </a> with great success.</p>
-<br>
+</font>
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-<br>
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-<br>
+<b>Flexibility</b><br><br>
-<br>
+</font>
-<br>
+<div class="image2">
-<br>
+<img src="https://static.igem.org/mediawiki/2011/a/aa/Plasmid_puzzle_colour.jpg" width="187px" height="50px"> </img><br>
 </div>
+<font color="#000000" face="arial" size="1" >
+<p align="justify">
+Standardization entails rigidity. Therefore we have written a <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization" >guide</a> that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.</p>
+</font>
+</div>
 <div class="yellow">
-<br>
+<font color="#000000" face="arial" size="3" >
-<br>
+<b>Applications</b><br><br>
-<br>
+</font>
-<br>
+<div class="image2">
-<br>
+<img src="https://static.igem.org/mediawiki/2011/8/8f/Application.jpg" width="187px" height="50px"> </img><br>
 </div>
-<br>
-<br>
-<br>
-<br>
+<font color="#000000" face="arial" size="1" >
-<div class="orange">
+<p align="justify">
-<ui>
+<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/introduction#Application%20area">Here</a> you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.</p>
-<li> We achieved to design a standardized cloning system, called Plug'n Play with DNA, for both mammalian cells and fungi. </li>
+</font>
-<li> We characterized proof of concept BioBrick with ß-galactosidase assays, Bradford assay and fluorescence. </li>
-<li> We demonstrated the function of our system i mammalian cells by fluorescence and confocal microscopy. </li>
-<li>We submitted 49 parts and 21 plasmids to the Registry of Standard Biological Parts <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/submitted biobricks"> List </a>.</li>
-<li>We applied for a gold medal by fulfilling the <a hret="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievement"> requirements listed</a> by iGEM 2011. </li>
-</ui>
 </div>
 <br>
 <br>
+<div class="purple">
+<font color="#000000" face="arial" size="3" >
+<b>Data Page</b><br><br>
+</font>
- <font color="#990000" face="arial" size="6" >
+<div class="image2">
-<b> Why Plug 'n' Play with DNA?</b><br>
-<br>
-  </font>
+<img src="https://static.igem.org/mediawiki/2011/c/c3/AspergillusDTU_billed.jpg" width="187px" height="50px"> </img><br>
+</div>
+<font color="#000000" face="arial" size="1" >
 <p align="justify">
-We have developed a standardized assembly system, called “Plug’n’Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs are custom made and can anneal to each other to form a stable hybridization product. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of pre-produced PCR-products will be distributed in microtiter plates directly ready for cloning. The simple and easy use of the system was demonstrated by developing a reporter targeting system for the mammalian cell line, U2OS, where GFP was targeted to the peroxisomes. In addition, application of our system was demonstrated in the fungus Aspergillus nidulans.
+You can find an overview of our project on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/data_page">Data Page</a>. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.</p>
-</p>
+</font>
+</div>
-</p>
+<div class="lightgreen">
+<font color="#000000" face="arial" size="3" >
+<b>Achievements</b><br><br>
+</font>
+<div class="image2">
+<img src="https://static.igem.org/mediawiki/2011/f/f7/Achievements.jpg" width="187px" height="50px"> </img><br>
+</div>
+<font color="#000000" face="arial" size="1" >
+<p align="justify">
+Designing a novel assembly standard and creating a reporter system for <i>Aspergillus nidulans</i> and mammalian cells are our main achievements. You can read more about our accomplishments on <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievements"> this page</a>.</p>
+</font>
+</div>
+<div class="pink">
+<font color="#000000" face="arial" size="3" >
+<b>The Team</b><br><br>
+</font>
+<img src="https://static.igem.org/mediawiki/2011/thumb/f/fd/DSC_0349.jpg/800px-DSC_0349.jpg" width="187px" height="50px"> </img><br>
+<font color="#000000" face="arial" size="1" >
+<p align="justify">
+We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/Team">Team Page</a>.</p>
+</font>
+</div>
   <td width="182px" height="100%" valign="top" >
@@ Line 244: / Line 353: @@
    slogan[9] = "Plug’n’play with DNA will change the world today";
    slogan[10] = "Plug’n’play can hold your favorite DNA";
-   slogan[11] = "Mix it, Heat it, Use it, it's that easy";
+   slogan[11] = "Mix it, heat it, use it, it's that easy";
    slogan[12] = "Avoid restriction, use the plug 'n' play edition";
-   slogan[13] = "Make a smart desision, use the plug 'n' play edition";
+   slogan[13] = "Make a smart decision, use the plug 'n' play edition";
    slogan[14] = "Plug’n’play with DNA is complete, it is easy, cool and smart, indeed";
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 <b> Sponsored by</b><br><br>
 </font>
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