Team:Freiburg/Notebook/17 September

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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 17 September </a>
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==<span style="color:black;">commons</span>==
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==<span style="color:red;">red light receptor</span>==
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===GST Pull-down Assay===
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===NAME OF YOUR EXPERIMENT===
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'''Investigators:Rüdiger, Julia, Sophie'''
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<br/>
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<br/>
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GFP-GST
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<br/>
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-Inoculate 1 colony in 3ml LB+Antibiotic on 37°C
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-next morning 1:250 dilution 2x250 ml ( in 1 liter flask)
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30°C shaking  for 3hours untill OD(600) is 0,4-06 <br/>
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'''Investigators:NAME'''
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==<span style="color:orange;">Lysis cassette</span>==
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===The little hoped for experiment II===
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'''Investigators:Theo'''
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==<span style="color:orange;">Lysis cassette</span>==
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the picked colonies were preped, but because GATC doesn't work on Saturdays I made a restriction plan to see if I had the much sought after composite part.. I would cut with EcoRI, EcoRV and PstI and if i had both parts I would get 4 easy to see bands... If you take the theoretical sequence of BBa_K608351 + BBa_K608352 you will see what I mean...
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Anyway it did not work, I could barely see a second band.
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===NAME OF YOUR EXPERIMENT===
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'''Investigators:NAME'''
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<br>
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on the new plate from yesterday there was a colony to be picked.. so I did exactly that
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==

Latest revision as of 00:19, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

commons

GST Pull-down Assay

Investigators:Rüdiger, Julia, Sophie

GFP-GST
-Inoculate 1 colony in 3ml LB+Antibiotic on 37°C -next morning 1:250 dilution 2x250 ml ( in 1 liter flask) 

30°C shaking  for 3hours untill OD(600) is 0,4-06

Lysis cassette

The little hoped for experiment II

Investigators:Theo


the picked colonies were preped, but because GATC doesn't work on Saturdays I made a restriction plan to see if I had the much sought after composite part.. I would cut with EcoRI, EcoRV and PstI and if i had both parts I would get 4 easy to see bands... If you take the theoretical sequence of BBa_K608351 + BBa_K608352 you will see what I mean... Anyway it did not work, I could barely see a second band.


on the new plate from yesterday there was a colony to be picked.. so I did exactly that

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/17_September"