Team:ULB-Brussels

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<a id="couleur" href="https://2011.igem.org/Team:ULB-Brussels">Home</a>
<a id="couleur" href="https://2011.igem.org/Team:ULB-Brussels">Home</a>
<a href="https://2011.igem.org/Team:ULB-Brussels/project">Project</a>
<a href="https://2011.igem.org/Team:ULB-Brussels/project">Project</a>
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<a href="https://2011.igem.org/Team:ULB-Brussels/modeling">Modeling</a>
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<a href="https://2011.igem.org/Team:ULB-Brussels/modeling">Modelling</a>
<a href="https://2011.igem.org/Team:ULB-Brussels/human">Human practice</a>
<a href="https://2011.igem.org/Team:ULB-Brussels/human">Human practice</a>
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<a href="https://2011.igem.org/Team:ULB-Brussels/Results">Results</a>
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<a href="https://2011.igem.org/Team:ULB-Brussels/Discussion">Discussion</a>
<a href="https://2011.igem.org/Team:ULB-Brussels/parts">Parts</a>
<a href="https://2011.igem.org/Team:ULB-Brussels/parts">Parts</a>
<a href="https://2011.igem.org/Team:ULB-Brussels/safety">Safety</a>
<a href="https://2011.igem.org/Team:ULB-Brussels/safety">Safety</a>
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One-step gene insertion or deletion
One-step gene insertion or deletion
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One of the most basic actions of all engineers is the assembly and the deletion of fundamental parts (bricks). Bearing in mind that one of the purposes of the iGEM is to make the link between synthetic biology and engineering sciences, we'd like to manage those simple steps in the easiest way in biological systems.  
One of the most basic actions of all engineers is the assembly and the deletion of fundamental parts (bricks). Bearing in mind that one of the purposes of the iGEM is to make the link between synthetic biology and engineering sciences, we'd like to manage those simple steps in the easiest way in biological systems.  
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Unfortunately, in E. coli, it's still difficult to do that in one step because of the lack of genetic tools to catalyze homologous recombination with linear DNA. By the assembly of a unique plasmid containing different genes derived from phages, and the design and construction of helper plasmids, we aim to provide the iGEM with a system that would confer to E. coli the useful properties of yeasts.  
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Unfortunately, in E. coli, it's still difficult to do that in one step because of the lack of genetic tools to catalyze homologous recombination with linear DNA. By the assembly of a unique plasmid containing different genes derived from phages, we aim to provide the iGEM with a system that would confer to E. coli the useful properties of yeasts.  
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In order to ensure high quality work, we build our project on three complementary and parallel axes :
In order to ensure high quality work, we build our project on three complementary and parallel axes :
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     <li>The first one is lead by a group called "Wet lab" and composed of biologists. They are charged to propose a design and to construct and experiment the tool for characterization.</li>
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     <li>The first one is lead by a group called "Wet lab" and composed of biologists. They are charged to propose a design and to construct and experiment the tool for characterisation.</li>
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     <li>The second one is based on a group called "Modeling Team" and composed of mathematicians and physicists. They model the genetic circuit with parameters derived from the characterization in order to find the optimal design and to refine the initial one.</li>
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     <li>The second one is based on a group called "Modelling Team" and composed of mathematicians and physicists. They model the genetic circuit with parameters derived from the characterisation in order to find the optimal design and to refine the initial one.</li>
     <li>And finally, the third one, organised by the "Human Practise team" composed of students from social sciences, will discuss the ethical questions asked by people when living organisms are being handled. They aim to understand people's fears with statistical survey, to give them inform them about synthetic biology and to check the impact of our intervention on their initial fears.</li>
     <li>And finally, the third one, organised by the "Human Practise team" composed of students from social sciences, will discuss the ethical questions asked by people when living organisms are being handled. They aim to understand people's fears with statistical survey, to give them inform them about synthetic biology and to check the impact of our intervention on their initial fears.</li>
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Sponsors
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Thanks for the support our sponsors have given us. <p>Without them the project would not have happened.</p>
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<a href="https://2011.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2011/a/a4/Igemlink.png" alt="igemhq" /></a>
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<p><strong> October 01 - 02 : </strong>European Jamborees</p>
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</br>
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<div id="facebook">
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<p><center> <strong>Full report:</strong>        <a href="http://91.121.27.129/Cam/FINAL2.pdf"><img src="https://static.igem.org/mediawiki/2011/0/07/PDF-Download.gif" alt="here" /></a></center>
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Join us on facebook
Join us on facebook
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Thanks for the support our sponsors have given us. <p>Without them the project would not have happened.</p></div>
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Next events
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<p><strong> September 01: </strong>Human practice debat in colaboration with KULeuven</p></br>
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<p><strong> September 21: </strong> Project and part documentation due </p></br>
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<p><strong>October 01 - 02 : </strong>European Jamborees</p></br>
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iGEM ULB Brussels Team - <a href="mailto:sgerard@ulb.ac.be">Contact us</a>
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iGEM ULB Brussels Team - <a href="mailto:lvmelder@ulb.ac.be">Contact us</a>
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Latest revision as of 03:48, 22 September 2011

One-step gene insertion or deletion

One of the most basic actions of all engineers is the assembly and the deletion of fundamental parts (bricks). Bearing in mind that one of the purposes of the iGEM is to make the link between synthetic biology and engineering sciences, we'd like to manage those simple steps in the easiest way in biological systems.

Unfortunately, in E. coli, it's still difficult to do that in one step because of the lack of genetic tools to catalyze homologous recombination with linear DNA. By the assembly of a unique plasmid containing different genes derived from phages, we aim to provide the iGEM with a system that would confer to E. coli the useful properties of yeasts.

We called this plasmid Pindel, acronym for plasmid of insertion and deletion of genes.

In order to ensure high quality work, we build our project on three complementary and parallel axes :

  • The first one is lead by a group called "Wet lab" and composed of biologists. They are charged to propose a design and to construct and experiment the tool for characterisation.
  • The second one is based on a group called "Modelling Team" and composed of mathematicians and physicists. They model the genetic circuit with parameters derived from the characterisation in order to find the optimal design and to refine the initial one.
  • And finally, the third one, organised by the "Human Practise team" composed of students from social sciences, will discuss the ethical questions asked by people when living organisms are being handled. They aim to understand people's fears with statistical survey, to give them inform them about synthetic biology and to check the impact of our intervention on their initial fears.

igemhq

October 01 - 02 : European Jamborees


Full report: here


Join us on facebook
iGEM ULB Brussels Team - Contact us

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