Team:Lyon-INSA-ENS/Project/Parts

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      <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" />
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         <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/IntroductionFr">Version Française</a>
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         <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/PartFr">Version Française</a>
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<div class="contenugrand2";>
<div class="contenugrand2";>
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<br/><br/>
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<br><br><br/><br/>
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      <p id="top"> <font color="green" size="6">
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              Data page <br><HR>
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              <br/>
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          </font>
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      </p>
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<p> <font color="green" size="5">
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               Rcn-csgBAEFG : placing the curli secretion under control of the cobalt-inducible promoter<br><HR>
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<ul style="list-style-type:circle;margin-left:10%;">       
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              <li> <a href="#rcn-csgBAEFG"> <font color="green"> <b style="line-height : 1.5em"> Overproduction of curli via a synthetic operon controled by a cobalt-inducible promoter P<i>rcn-csgBAEFG</i> </b> </font> </a> </li>
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               <br/>
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      <li> <a href="#ompR"> <font color="green"> <b>Overexpression of curli genes via the superactivator OmpR234  </b> </font> </a> </li>
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              <br>
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              <li> <a href="#parts"> <font color="green"> <b> Our parts </b> </font> </a> </li>
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              <br/>
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        </ul>
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<br/><br/><br/>
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        <p id="rcn-csgBAEFG"> <font color="green" size="4" style="line-height : 1.5em">
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              <big>Overproduction of curli </big>via a synthetic operon controled by a cobalt-<br>inducible promoter P<i>rcn-csgBAEFG</i><br><HR>
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          </font></p>
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<br> <br>
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<p>
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Hover the mouse over the numbers to see the stages in detail.
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<br> <br>
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</p>
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<div style="text-align:center;">
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</div>
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<br><br><br><br><br>
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        <p id="ompR"> <font color="green" size="4">
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              <big>Overexpression of curli genes</big> via the superactivator OmpR234<br><HR>
           </font></p>
           </font></p>
<br> <br><br> <br>
<br> <br><br> <br>
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<p>
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Hover the mouse over the numbers to see the stages in detail.
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<br> <br>
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</p>
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<div style="text-align:center;">
<div style="text-align:center;">
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<img src="https://static.igem.org/mediawiki/2011/4/42/Rcncsg_mecanisme_schema.jpg"/ heigth="350px" width="700px">
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<br><br>
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<br>
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<br>
     <br/><br/><br/><br/><br/>
     <br/><br/><br/><br/><br/>
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         <p> <font color="green" size="5">
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         <p id="parts"> <font color="green" size="6">
               Our parts<br><HR>
               Our parts<br><HR>
           </font></p>
           </font></p>
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<p style="line-height:1.5em">
<p style="line-height:1.5em">
<b>Data for our favorite new parts </b> <br/><br/>
<b>Data for our favorite new parts </b> <br/><br/>
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1.<a href="http://partsregistry.org/Part:BBa_K540000">Main page</a> - <b>rcn-csgBAEFG, cobalt-inducible promoter controlling a curli-generating gene, BBa_K540000</b>. This part enable production of cobalt induced curlis, which induces the formation of a biofilm.<br/>
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1.<a href="http://partsregistry.org/Part:BBa_K540000">Main page</a> - <b><i>rcn-csgBAEFG</i>, cobalt-inducible promoter controlling a curli-generating gene, BBa_K540000</b>. This part enable production of cobalt induced curlis, which induces the formation of a biofilm.<br/>
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2.<a href="http://partsregistry.org/Part:BBa_K540001">Main page</a> - <b>rcn, cobalt-inducible promoter, BBa_K540001 </b>. This promoter increases the production of a gfp placed under its control when cobalt is in the medium.<br/>
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2.<a href="http://partsregistry.org/Part:BBa_K540001">Main page</a> - <b><i>rcn</i>, cobalt-inducible promoter, BBa_K540001 </b>. This promoter increases the production of a gfp placed under its control when cobalt is in the medium.<br/>
</p>
</p>
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<p style="line-height:1.5em">
<p style="line-height:1.5em">
<b>Data for pre-existing parts </b> <br/><br/>
<b>Data for pre-existing parts </b> <br/><br/>
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1.<a href="http://partsregistry.org/Part:BBa_K342003">Main page</a> - <b>OmpR234, a stimulating gene for curli production, BBa_K342003 </b> This part has been shown less efficient than csgBAEFG to induce adherence.<br/>
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1.<a href="http://partsregistry.org/Part:BBa_K342003">Main page</a> - <b><i>ompR234</i>, a stimulating gene for curli production, BBa_K342003 </b> This part has been shown to induce adherence as well.<br/>
</p>
</p>

Latest revision as of 07:39, 21 September 2011




Stage 1 Stage 2 Stage 3 Stage 4 Stage 5 Stage 1 Stage 2 Stage 3




Data page





Overproduction of curli via a synthetic operon controled by a cobalt-
inducible promoter Prcn-csgBAEFG



Hover the mouse over the numbers to see the stages in detail.






Overexpression of curli genes via the superactivator OmpR234





Hover the mouse over the numbers to see the stages in detail.










Our parts




Data for our favorite new parts

1.Main page - rcn-csgBAEFG, cobalt-inducible promoter controlling a curli-generating gene, BBa_K540000. This part enable production of cobalt induced curlis, which induces the formation of a biofilm.
2.Main page - rcn, cobalt-inducible promoter, BBa_K540001 . This promoter increases the production of a gfp placed under its control when cobalt is in the medium.



Data for pre-existing parts

1.Main page - ompR234, a stimulating gene for curli production, BBa_K342003 This part has been shown to induce adherence as well.












ENS assystem Biomérieux INSA INSA
Retrieved from "http://2011.igem.org/Team:Lyon-INSA-ENS/Project/Parts"