Team:Potsdam Bioware

From 2011.igem.org

(Difference between revisions)
(→Safety and Security Assessment)
 
(72 intermediate revisions not shown)
Line 2: Line 2:
<html>
<html>
-
<div class="div_home">
+
<div class="div_home" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Project/Summary','_self');">
-
<p>
+
-
<!--
 
-
<b>Hallo liebes iGEM Potsdam Team :P , auch wenn ihr unser Wiki gemopst habt, wünschen wir euch viel Erfolg beim Endspurt ;)</b>
 
-
-->
 
<h2>Modification, Selection and Production of Cyclic Peptides for Therapy</h2>
<h2>Modification, Selection and Production of Cyclic Peptides for Therapy</h2>
 +
<table cellpadding="0"><td><img src="https://static.igem.org/mediawiki/2011/7/7d/UP_micrviridin_movie_final.gif"/></td>
 +
<td><p style="text-align:justify;">One key task of biopharmaceuticals is the binding and blocking of deregulated proteins. Towards this goal, we mutate and select microviridins, which are tricyclic depsipeptides from cyanobacteria. They are small but stable due to their post-translational side-chain crosslinking. Microviridins have a high potential for therapy as they can block disease-relevant proteases. Yet, the possibilities of cyclic peptides are largely untapped since genetic systems for optimization are not well established. Thus, we developed synthetic systems for the mutation, selection and production of such peptides. We utilized the 6.5 kb microviridin (<i>mdn</i>) gene cluster cloned in <i>E. coli</i> plasmids, established random mutagenesis and generated focused libraries of microviridins. For selection against a panel of proteases, we are applying and testing phage display, and we are constructing a novel in-vivo selection device, which links protease blocking to antibiotic resistance. Our systems adhere to the BioBrick standards. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Project/Summary"><span class="bold">[more]</span></a></p>
 +
</td></table>
-
<p style="text-align:justify;">One key task of biopharmaceuticals is the binding and blocking of deregulated proteins. Towards this goal, we mutate and select microviridins, which are tricyclic depsipeptides from cyanobacteria. They are small but stable due to their post-translational side-chain crosslinking. Microviridins have a high potential for therapy as they can block disease-relevant proteases. Yet, the possibilities of cyclic peptides are largely untapped since genetic systems for optimization are not well established. Thus, we developed synthetic systems for the mutation, selection and production of such peptides. We use the 6.5 kb microviridin (mdn) gene cluster cloned in E. coli plasmids, established random mutagenesis and generated focused libraries of microviridins. For selection against a panel of proteases, we are applying and testing phage display, and we are constructing a novel in-vivo selection device, which links protease blocking to antibiotic resistance. Our systems, including the 6.5 kb cluster, adhere to the BioBrick standards.
 
-
</p>
 
-
</p>
 
</div>
</div>
-
<!---Box on the upper left: Project--->
+
<!---Box on the upper left: Software--->
-
<div class="box_home">
+
<div class="box_home" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Software','_self');">
-
<h2>Project</h2>
+
<img id="topRight" src="https://static.igem.org/mediawiki/2011/b/bb/UP_home_Software1.png">
 +
<h2>Software</h2>
<p>
<p>
-
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Project/Results"><span class="bold">[more]</span></a></p>
+
<b>BioLog</b> – The new allround talent in the lab for your smart phone! Our app combines several features frequently used in the lab. The software stores basic protocols in your pocket - easy, handy and ready to use… <a href="https://2011.igem.org/Team:Potsdam_Bioware/Software"><span class="bold">[more]</span></a></p>
</div>
</div>
-
<!---Small box on the right: Team--->
+
<!---Small box on the right: Team
<div class="box_long">
<div class="box_long">
Line 34: Line 31:
</div>
</div>
-
<!--- News Ticker --->
+
-->
-
<div class="box_long box_long_news news_ticker">
+
<!--- Team --->
-
<a href="https://2011.igem.org/Team:Potsdam_Bioware/NoteBook/Statistics" style="z-index:20;position:relative;"><h2>Statistics - In a Minute</h2></a>
+
 
 +
<div class="box_home box_long_news news_ticker" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Team','_self');">
 +
<a href="https://2011.igem.org/Team:Potsdam_Bioware/Team" style="z-index:20;position:relative;"><h2>Team</h2></a>
<ul id="news" class="newsticker">
<ul id="news" class="newsticker">
<li><div class="news">
<li><div class="news">
-
   <img src="https://static.igem.org/mediawiki/2010/a/a5/Freiburg10_Longest_Wikipage.png" alt="Lorem Ipsum 1" width="368">
+
   <img src="https://static.igem.org/mediawiki/2011/6/67/UP_Nicole.jpg" alt="Nicole Albrecht"/>
-
     <p>Lorem Ipsum 1:<br/>
+
     <p>Nicole Albrecht</p>
-
        1
+
-
    </p>
+
</div>
</div>
</li>
</li>
-
<li><div class="news"><img
+
<li><div class="news">
-
src="https://static.igem.org/mediawiki/2010/a/a5/Freiburg10_Longest_Wikipage.png"
+
    <img src="https://static.igem.org/mediawiki/2011/1/13/UP_Katharina.jpg" alt="Katharina Berger" />
-
alt="Lorem Ipsum 2" width="368"><p>Lorem Ipsum 2:<br />2</p></div>
+
    <p>Katharina Berger</p>
 +
</div>
</li>
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/0/0e/UP_Nadja.jpg" alt="Nadja Bjelopoljak" />
 +
    <p>Nadja Bjelopoljak</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/e/e4/UP_Nadine.jpg" alt="Nadine Boehmer" />
 +
    <p>Nadine Boehmer</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/8/82/UP_VanessaB.jpg" alt="Vanessa Boehmer" />
 +
    <p>Vanessa Boehmer</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/a/a3/UP_Jessica.jpg" alt="Jessica Eger" />
 +
    <p>Jessica Eger</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/b/b1/UP_Steffi.jpg" alt="Steffi Sempert" />
 +
    <p>Steffi Sempert</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/e/ed/UP_Niels.jpg" alt="Niels Weisbach" />
 +
    <p>Niels Weisbach</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/b/b0/UP_Sebastian.jpg" alt="Sebastian Hanke" />
 +
    <p>Sebastian Hanke</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/d/d1/UP_SaschaR.jpg" alt="Sascha Ramm" />
 +
    <p>Sascha Ramm</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/8/87/UP_Paul.jpg" alt="Paul Kaufmann" />
 +
    <p>Paul Kaufmann</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/c/cb/UP_Stefan.jpg" alt="Stefan Wahlefeld" />
 +
    <p>Stefan Wahlefeld</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/6/66/UP_Sandrina.jpg" alt="Sandrina Heyde" />
 +
    <p>Sandrina Heyde</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/3/37/UP_Sabine.jpg" alt="Sabine Meyer" />
 +
    <p>Sabine Meyer</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/e/eb/UP_Niklas.jpg" alt="Niklas Laasch" />
 +
    <p>Niklas Laasch</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
<img src="https://static.igem.org/mediawiki/2011/c/c0/UP_Oliver.jpg" alt="Oliver Zimmer" />
 +
    <p>Oliver Zimmer</p>
 +
</div>
 +
</li>
 +
 +
</li>
 +
<li><div class="news">
 +
    <img src="https://static.igem.org/mediawiki/2011/5/5b/UP_TobiasW.jpg" alt="Tobias Wenzel" />
 +
    <p>Tobias Wenzel</p>
 +
</div>
 +
</li>
 +
</ul>
</ul>
</div>
</div>
Line 56: Line 160:
<!---Box on the upper right: BioBricks--->
<!---Box on the upper right: BioBricks--->
<div height="290px">
<div height="290px">
-
<div class="box_home_small">
+
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Data_Page','_self');">
 +
<img class="right" src="https://static.igem.org/mediawiki/2011/8/84/UP_home_BioBricks.png"/>
<h2>BioBricks</h2>
<h2>BioBricks</h2>
-
<p class="standard">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua.<a href="https://2011.igem.org/Team:Potsdam_Bioware/BioBricks"><span class="bold">[more]</a></span></p>
+
<p class="standard">
 +
Our datapage displays our systems and BioBricks in a nutshell. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Data_Page"><span class="bold">[more]</a></span><br> <br>
 +
Our BioBricks are top-down for the <i>mdn</i> gene cluster and botton-up for detection and selection. <a href="https://2011.igem.org/Team:Potsdam_Bioware/BioBricks"><span class="bold">[more]</a></span></p>
</div>
</div>
-
<!---Box on the lower left: Safety, Ethics--->
+
<!---Box on the lower left: Human Practice--->
-
<div class="box_home_small">
+
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Safety_Ethics','_self');">
-
<h2>Safety &amp; Ethics</h2></a>
+
<img class="right" src="https://static.igem.org/mediawiki/2011/1/19/UP_home_Safety.png"/>
-
<p class="standard">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Safety_ethics"><span class="bold">[more]</span></a></p>
+
<h2>Safety & Ethics</h2></a>
 +
<p class="standard">We discussed safety issues and ethic controversies in seminars and polled all members of the German parliament for their opinion on synthetic biology. Learn more about German views. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Safety_Ethics"><span class="bold">[more]</span></a></p>
</div>
</div>
<!---Box on the lower right: labjournal--->
<!---Box on the lower right: labjournal--->
-
<div class="box_home_small">
+
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Labjournal','_self');">
 +
<img class="right" src="https://static.igem.org/mediawiki/2011/2/2a/UP_home_Labjournal.png"/>
<h2>Labjournal</h2>
<h2>Labjournal</h2>
<p>
<p>
-
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua.<a href="https://2011.igem.org/Team:Potsdam_Bioware/journal/journal"><span class="bold">[more]</span></a></p>
+
All the hours spent in the lab - thinking, laughing, sweating and hoping. Here is the tour guide through our lab work. We hope, you enjoy the trip. Please fasten your seat belt and mind the gap. <a href="https://2011.igem.org/Team:Potsdam_Bioware/Labjournal"><span class="bold">[more]</span></a></p>
</div>
</div>
-
<!---Box on the lower right: Software--->
+
<!---Box on the lower right: Modeling--->
-
<div class="box_home_small">
+
<div class="box_home_small" onClick="window.open('https://2011.igem.org/Team:Potsdam_Bioware/Project/Summary#Modeling','_self');">
-
<h2>Software</h2></a>
+
<img class="right" src="https://static.igem.org/mediawiki/2011/b/bd/UP_home_Modeling.png"/>
-
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua.
+
<h2>Modeling</h2></a>
-
<a href="https://2011.igem.org/Team:Potsdam_Bioware/software"><span class="bold">[more]</span></a></p>
+
<p class="standard">
 +
This year we focused on systems modeling in which the reaction kinetics of the <i>in vivo</i> selection are analyzed. Read more about the predictions we were able to derive from our model.
 +
<a href="https://2011.igem.org/Team:Potsdam_Bioware/Project/Summary#Modeling"><span class="bold">[more]</span></a></p>
</div>
</div>
</div>
</div>
<div class="clearfix"></div>
<div class="clearfix"></div>
-
 
+
<!--
-
</html>
+
-
 
+
-
 
+
-
 
+
-
 
+
===Labjournal===
===Labjournal===
<li>[[labjournal_may| May]]
<li>[[labjournal_may| May]]
Line 98: Line 204:
<li>[[labjournal_september|September]]
<li>[[labjournal_september|September]]
 +
-->
 +
</html>
-
<html><center><div id="clustrmaps-widget"></div><script type="text/javascript">var _clustrmaps = {'url' : 'https://2011.igem.org/Team:Potsdam_Bioware', 'user' : 910340, 'server' : '2', 'id' : 'clustrmaps-widget', 'version' : 1, 'date' : '2011-07-15', 'lang' : 'en', 'corners' : 'square' };(function (){ var s = document.createElement('script'); s.type = 'text/javascript'; s.async = true; s.src = 'http://www2.clustrmaps.com/counter/map.js'; var x = document.getElementsByTagName('script')[0]; x.parentNode.insertBefore(s, x);})();</script><noscript><a href="http://www2.clustrmaps.com/user/239de404"><img src="http://www2.clustrmaps.com/stats/maps-no_clusters/2011.igem.org-Team-Potsdam_Bioware-thumb.jpg" alt="Locations of visitors to this page" /></a></noscript><center></html>
 
-
 
-
 
-
 
-
 
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert ***
 
-
 
-
<html>
 
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
 
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
 
-
This is a template page. READ THESE INSTRUCTIONS.
 
-
</div>
 
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
-
</div>
 
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
 
-
 
 
-
</div>
 
-
</div>
 
-
 
-
 
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace.
 
-
 
-
{|align="justify"
 
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
-
|[[Image:Potsdam_Bioware_logo.png|200px|right|frame]]
 
-
|-
 
-
|
 
-
''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
-
|[[Image:Potsdam_Bioware_team.png|right|frame|Your team picture]]
 
-
|-
 
-
|
 
-
|align="center"|[[Team:Potsdam_Bioware | Team Example]]
 
-
|}
 
 +
<html><center>
 +
<div id="clustrmaps-widget"></div><script type="text/javascript">var _clustrmaps = {'url' : 'https://2011.igem.org/Team:Potsdam_Bioware', 'user' : 910340, 'server' : '2', 'id' : 'clustrmaps-widget', 'version' : 1, 'date' : '2011-07-15', 'lang' : 'en', 'corners' : 'square' };(function (){ var s = document.createElement('script'); s.type = 'text/javascript'; s.async = true; s.src = 'http://www2.clustrmaps.com/counter/map.js'; var x = document.getElementsByTagName('script')[0]; x.parentNode.insertBefore(s, x);})();</script><noscript><a href="http://www2.clustrmaps.com/user/239de404"><img src="http://www2.clustrmaps.com/stats/maps-no_clusters/2011.igem.org-Team-Potsdam_Bioware-thumb.jpg" alt="Locations of visitors to this page" /></a></noscript>
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<p style="font-size:xx-small; text-align:center; color:grey">primary contact: Kristian Müller, kristian@syntbio.net, http://www.syntbio.net</p>
-
!align="center"|[[Team:Potsdam_Bioware|Home]]
+
</center></html>
-
!align="center"|[[Team:Potsdam_Bioware/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Potsdam_Bioware Official Team Profile]
+
-
!align="center"|[[Team:Potsdam_Bioware/Project|Project]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Modeling|Modeling]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Notebook|Notebook]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Safety|Safety]]
+
-
!align="center"|[[Team:Potsdam_Bioware/Attributions|Attributions]]
+
-
|}
+
-
</html>  -->
+

Latest revision as of 13:38, 16 November 2011

Modification, Selection and Production of Cyclic Peptides for Therapy

One key task of biopharmaceuticals is the binding and blocking of deregulated proteins. Towards this goal, we mutate and select microviridins, which are tricyclic depsipeptides from cyanobacteria. They are small but stable due to their post-translational side-chain crosslinking. Microviridins have a high potential for therapy as they can block disease-relevant proteases. Yet, the possibilities of cyclic peptides are largely untapped since genetic systems for optimization are not well established. Thus, we developed synthetic systems for the mutation, selection and production of such peptides. We utilized the 6.5 kb microviridin (mdn) gene cluster cloned in E. coli plasmids, established random mutagenesis and generated focused libraries of microviridins. For selection against a panel of proteases, we are applying and testing phage display, and we are constructing a novel in-vivo selection device, which links protease blocking to antibiotic resistance. Our systems adhere to the BioBrick standards. [more]

Software

BioLog – The new allround talent in the lab for your smart phone! Our app combines several features frequently used in the lab. The software stores basic protocols in your pocket - easy, handy and ready to use… [more]

Team
  • Nicole Albrecht

    Nicole Albrecht

  • Katharina Berger

    Katharina Berger

  • Nadja Bjelopoljak

    Nadja Bjelopoljak

  • Nadine Boehmer

    Nadine Boehmer

  • Vanessa Boehmer

    Vanessa Boehmer

  • Jessica Eger

    Jessica Eger

  • Steffi Sempert

    Steffi Sempert

  • Niels Weisbach

    Niels Weisbach

  • Sebastian Hanke

    Sebastian Hanke

  • Sascha Ramm

    Sascha Ramm

  • Paul Kaufmann

    Paul Kaufmann

  • Stefan Wahlefeld

    Stefan Wahlefeld

  • Sandrina Heyde

    Sandrina Heyde

  • Sabine Meyer

    Sabine Meyer

  • Niklas Laasch

    Niklas Laasch

  • Oliver Zimmer

    Oliver Zimmer

  • Tobias Wenzel

    Tobias Wenzel

BioBricks

Our datapage displays our systems and BioBricks in a nutshell. [more]

Our BioBricks are top-down for the mdn gene cluster and botton-up for detection and selection. [more]

Safety & Ethics

We discussed safety issues and ethic controversies in seminars and polled all members of the German parliament for their opinion on synthetic biology. Learn more about German views. [more]

Labjournal

All the hours spent in the lab - thinking, laughing, sweating and hoping. Here is the tour guide through our lab work. We hope, you enjoy the trip. Please fasten your seat belt and mind the gap. [more]

Modeling

This year we focused on systems modeling in which the reaction kinetics of the in vivo selection are analyzed. Read more about the predictions we were able to derive from our model. [more]


primary contact: Kristian Müller, kristian@syntbio.net, http://www.syntbio.net

Retrieved from "http://2011.igem.org/Team:Potsdam_Bioware"